The distribution of IL-13 receptor α1 expression on B cells, T cells and monocytes and its regulation by IL-13 and IL-4

Abstract

To study the expression of IL-13 receptor § 1 (IL-13R § 1), specific monoclonal antibodies (mAb) were generated. Surface expression of the IL-13R § 1 on B cells, monocytes and T cells was assessed by flow cytometry using these specific mAb. Among tonsillar B cells, the expression was the highest on the IgD + CD38-B cell subpopulation which is believed to represent naive B cells. Expression was also detectable on a large fraction of the IgD-CD38-B cells but not on CD38 + B cells. Activation under conditions which promote B cell Ig class switching up-regulated the expression of the receptor. However, the same stimuli had an opposite effect for IL-13R § 1 expression levels on monocytes. While IL-13R § 1 mRNA was clearly detectable in T cell preparations, no surface expression was detected. However, per-meabilization of the T cells showed a clear intracellular expression of the receptor. A soluble form of the receptor was immunoprecipitated from the supernatant of activated peripheral T cells, suggesting that T cell IL-13R § 1 might have functions unrelated to the capacity to form a type II IL-4/IL-13R with IL-4R § .

Novel Diversity in IL-4-Mediated Responses in Resting Human Naive B Cells Versus Germinal Center/Memory B Cells

“…One explanation for normal B cell survival and maintenance in the absence of IL-4 is the functional presence of IL-13. Interestingly, the expression of IL-13 receptor ␣1-chain was found to be the highest on naive B cells (51). Thus, simultaneous disruption of IL-4, IL-13, and other redundant B cell cytokines may result in poor B cell viability, in particular naive B cells.…”

Interleukin-13 Fusion Cytotoxin Arrests Schistosoma mansoni Egg-Induced Pulmonary Granuloma Formation in Mice

“…10 IL-4 and IL-13 receptors are expressed on a variety of immune and nonimmune cells including B cells, natural killer cells, monocytes, mast cells, endothelial cells, and fibroblasts, 8,11,12 but naïve and memory T cells do not appear to respond to the extracellular presence of IL-13. 13,14 Previous studies have shown that the granulomatous response elicited by Schistosoma mansoni via infection [15][16][17][18][19][20] after intravenous injection of live S. mansoni eggs 16,[21][22][23][24][25][26] or after intravenous injection of S. mansoni egg-antigen-coated Sephadex beads 27,28 is dependent on the Th2-dominant cellular events mediated by IL-4 and IL-13. These previous findings were derived from studies that used cytokine immunoneutralization, gene-knockout technology, or receptor decoy strategies designed to target IL-4, IL-13, both cytokines, or their receptors.…”

Ku autoantigen (DNA helicase) is required for interleukins-13/-4-induction of 15-Lipoxygenase-1 gene expression in human epithelial cells

“…These binding proteins were then concentrated by ultrafiltration (Amicon YM10 membrane) and stored in liquid nitrogen until further use. 4 and either anti-Ku70, anti-Ku80, Ku70/80 or control IgG1 antibodies at a final concentration of 10 g/ml were used. Following electroporation, cells were incubated for an additional 30 min at 37°C and 5% CO 2 , washed once with serum-free F-12 nutrient mixture (HAM) medium, and then cultured in HAM medium with serum during the stimulation with IL-13 and IL-4.…”

“…Like IL-4, IL-13 is a regulator of human B cell and monocyte functions. 4 Recently Yu et al 5 demonstrated that IL-13 induces distinct STAT6-DNA binding complexes and tyrosine phosphorylation of STAT6 and Janus kinase 3 (JAK3) in NK and T cells. Curiel et al 6 have identified a Stat-6-responsive element (Stat-6RE) in the promoter of the human IL-4 gene, as well as two specific IL-4 responsive DNA-protein complexes in nuclear extracts of both human Th1 and Th2 clones.…”