The distribution of an AVT V1a receptor in the brain of a sex changing fish, Epinephelus adscensionis

Kline, Richard J.; O’Connell, Lauren A.; Hofmann, Hans A.; Holt, G. Joan; Khan, Izhar Ahmad

doi:10.1016/j.jchemneu.2011.06.005

Cited by 57 publications

(47 citation statements)

References 60 publications

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“…Though the interaction between 5-HT and AVT has not been extensively analyzed in teleosts, raphe neurons expressing AVT receptors have been recently described (Kline et al, 2011). Following the same trend reported in mammals, we observed opposing actions of 5-HT and AVT upon overt aggression in the territorial aggression of G. omarorum, probably acting by enhancing and decreasing the activity of the same target areas across the SBN (Fig.6).…”

Section: Conclusion and Speculationssupporting

confidence: 82%

Neuromodulation of the agonistic behavior in two species of weakly electric fish that display different types of aggression

Silva

Perrone

Zubizarreta

et al. 2013

Journal of Experimental Biology

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SummaryAgonistic behavior has shaped sociality across evolution. Though extremely diverse in types of displays and timing, agonistic encounters always follow the same conserved phases (evaluation, contest and post-resolution) and depend on homologous neural circuits modulated by the same neuroendocrine mediators across vertebrates. Among neuromodulators, serotonin (5-HT) is the main inhibitor of aggression, and arginine vasotocin (AVT) underlies sexual, individual and social context differences in behavior across vertebrate taxa. We aim to demonstrate that a distinct spatio-temporal pattern of activation of the social behavior network characterizes each type of aggression by exploring its modulation by both the 5-HT and AVT systems. We analyze the neuromodulation of aggression between the intermale reproduction-related aggression displayed by the gregarious Brachyhypopomus gauderio and the non-breeding intrasexual and intersexual territorial aggression displayed by the solitary Gymnotus omarorum. Differences in the telencephalic activity of 5-HT between species were paralleled by a differential serotonergic modulation through 1A receptors that inhibited aggression in the territorial aggression of G. omarorum but not in the reproduction-related aggression of B. gauderio. AVT injection increased the motivation towards aggression in the territorial aggression of G. omarorum but not in the reproduction-related aggression of B. gauderio, whereas the electric submission and dominance observed in G. omarorum and B. gauderio, respectively, were both AVT-dependent in a distinctive way. The advantages of our model species allowed us to identify precise target areas and mechanisms of the neuromodulation of two types of aggression that may represent more general and conserved strategies of the control of social behavior among vertebrates.

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Section: Conclusion and Speculationssupporting

confidence: 82%

Neuromodulation of the agonistic behavior in two species of weakly electric fish that display different types of aggression

Silva

Perrone

Zubizarreta

et al. 2013

Journal of Experimental Biology

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“…Although an AVT neuronal population has never been described in the olfactory bulbs of teleosts, V1a receptors have been identified in this area (Kline et al, 2011), which together with the high AVT peptide levels reported here, suggest a role for AVT in the processing of olfactory stimuli in fish. The Mozambique tilapia may prove to be an excellent model species for investigating the AVT modulation of olfactory-based social communication in fish, since urine-born compounds are actively used both in agonistic male-male interactions and during courtship in this species (Almeida et al, 2005;Barata et al, 2007;Frade et al, 2002).…”

Section: Discussionmentioning

confidence: 45%

“…In fish these nonapeptides are produced by neurons localized in the parvocellular and magnocellular nuclei of the preoptic area, that project both to the neurohypophysis, where AVT/IT are released in the systemic circulation, and to multiple extrahypothalamic regions, namely the ventral telencephalon, the thalamus and the mesencephalon (Goodson and Bass, 2001;Goodson et al, 2003;Holmqvist and Ekstrom, 1995;Thompson and Walton, 2009). The functionality of the wide extrahypothalamic projections is confirmed by the presence of AVT/IT receptors in most brain regions, in particular the AVT V1a receptor (Hausmann et al, 1995;Kline et al, 2011;Lema, 2010;Lema et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Brain levels of arginine–vasotocin and isotocin in dominant and subordinate males of a cichlid fish

Almeida

Gozdowska

Kulczykowska

et al. 2012

Hormones and Behavior

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The nonapeptides arginine-vasotocin (AVT) and isotocin (IT), which are the teleost homologues of argininevasopressin and oxytocin in mammals, have well established peripheral effects on osmoregulation and stress response, and central effects on social behavior. However, all studies that have looked so far into the relationship between these nonapeptides and social behavior have used indirect measures of AVT/IT activity (i.e. immunohistochemistry of AVT/IT immunoreactive neurons, or AVT/IT or their receptors mRNA expression with in situ hybridization or qPCR) and therefore direct measures of peptide levels in relation to social behavior are still lacking. Here we use a recently developed high-performance liquid chromatography analysis with fluorescence detection (HPLC-FL) method to quantify the levels of both AVT and IT in macro-dissected brain areas [i.e. olfactory bulbs, telencephalon, diencephalon, optic tectum, cerebellum, and hindbrain (= rhombencephalon minus cerebellum)] and pituitary of dominant and subordinate male cichlid fish (Oreochromis mossambicus). The pituitary shows higher levels of both peptides than any of the brain macroareas, and the olfactory bulbs have the highest AVT among all brain areas. Except for IT in the telencephalon there is a lack of correlations between central levels and pituitary peptide levels, suggesting an independent control of hypophysial and CNS nonapeptide secretion. There were also no correlations between AVT and IT levels either for each brain region or for the pituitary gland, suggesting a decoupled activity of the AVT and IT systems at the CNS level. Subordinate AVT pituitary levels are significantly higher than those of dominants, and dominant hindbrain IT levels are significantly higher than those of subordinates, suggesting a potential involvement of AVT in social stress in subordinate fish and of IT in the regulation of dominant behavior at the level of the hindbrain. Since in this species dominant males use urine to communicate social status and since AVT is known to have an antidiuretic effect, we have also investigated the effect of social status on urine storage. As predicted, dominant males stored significantly more urine than subordinates. Given these results we suggest that AVT/IT play a key role in orchestrating social phenotypes, acting both as central neuromodulators that promote behavioral plasticity and as peripheral hormones that promote integrated physiological changes.

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“…These three genes have been previously cloned in the pupfish C. n. amargosae (Lema 2010). Subsequently, both v1a1 and v1a2 subtypes were cloned in Epinephalus (Kline et al 2011), bluehead wrasse (Lema et al 2012) and zebrafish (Iwasaki et al 2013 southern platyfish, four subtypes of V2R have been described (V2A1, V2A2, V2B and V2C). In the southern platyfish, the V2B has duplicates 1 and 2 so that the total number of the V2R is 5.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

Rawat

Chaube

Joy

2015

Fish Physiol Biochem

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Vasotocin (VT) is the ortholog of vasopressin (VP) in non-mammalian vertebrates and is known for multiple functions. Teleost fishes have a complete repertoire of known VP/VT receptor subtypes (vasopressin type, VR): two V1A subtypes (V1Aa and V1Ab or V1a1 and V1a2) and five V2 subtypes (V2A1, V1A2, V2B1, V2B2 and V2C). Full-length cDNAs of v1a1, v1a2 and v2 (v2a1) with ORFs of 1,308, 1,137 and 1,527 bp, respectively, were cloned and characterized in the catfish Heteropneustes fossilis (Siluriformes, Ostariophysi). BLAST analysis revealed that the genes encoded three VT receptors, V1a1, V1a2 and V2 of 436, 379 and 509 amino acid residues, respectively. The predicted proteins showed typical features of the seven-transmembrane domain receptor core structure with hallmark triplets Asp-Arg-Tyr/Asp-Arg-His (DRY/DRH) and the variable intracellular loop III of vertebrate neurohypophysial hormone receptors. Phylogenetic analysis of the deduced protein sequences revealed that they clustered with the V1Aa, V1Ab and V2A1, respectively, of other teleosts. The V2R has a sequence identity of 70-76% with V2A1 than with the V2B type (sequence identity 43-49%). Semiquantitative PCR analysis showed that the receptor gene transcripts were expressed ubiquitously in the tissues examined (brain, pituitary, gonads, liver, muscle, kidney and gills) and displayed sex and seasonal fluctuations in a tissue-specific manner. The results form a basis for functional studies on the VT receptors in the catfish.

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The distribution of an AVT V1a receptor in the brain of a sex changing fish, Epinephelus adscensionis

Cited by 57 publications

References 60 publications

Neuromodulation of the agonistic behavior in two species of weakly electric fish that display different types of aggression

Neuromodulation of the agonistic behavior in two species of weakly electric fish that display different types of aggression

Brain levels of arginine–vasotocin and isotocin in dominant and subordinate males of a cichlid fish

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

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