The Distribution of 51cr-Labeled Syngeneic and Allogeneic Lymph Node Cells in the Rat

Heslop, Barbara F.; Hardy, Britta

doi:10.1097/00007890-197102000-00004

Cited by 45 publications

(23 citation statements)

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“…Similar observations were later done in the rat (Heslop and Hardy 1971) and sheep (Frost et al 1975). injected allogeneic 51-chromium labelled lymphocytes, observed that lymphocytes from some MHC-incompatible mice strains disappeared more rapidly from the lymphoid tissues of the recipient mice than could be accounted for by adaptive immune responses.…”

Section: Introduction To the Concept Of Auogeneic Lymphocyte Cytotoxisupporting

confidence: 77%

Non-adaptive cellular immune responses as studied in euthymic and athymic nude rats

Rolstad

Fossum

1990

Anat Embryol

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The athymic nude (rnu) rat lacks a functional thymus and normal alloreactive T cells. These animals, therefore, have been widely used as tools for studying thymus-independent immune responses. The absence of functional T cells would, to most investigators, indicate that these rats have a defective cellular immune defence. However, although rnu rats accept organ allografts infinitely, they are nevertheless capable of rejecting allografts consisting of lymphocytes or bone marrow cells with increased vigour, and this via antibody-independent mechanisms. These rejection phenomena have operationally been termed allogeneic lymphocyte cytotoxicity (ALC) and allogeneic bone marrow cell cytotoxicity (ABC). Unlike organ allograft immunity this kind of rejection requires no presensitization of the recipient and is surprisingly rapid: it commences within a few hours of i.v. injection of the allogeneic cells and is usually complete by 24 h. Moreover, products coded for by genes within, or closely linked to the major histocompatibility complex (MHC), are clearly involved in the interaction between effector and target cells, as grafted cells from MHC-congenic rat strains are vigorously rejected. In contrast to the defective T cell immune responses in athymic nude rats, the natural killer (NK) cell function is not impaired, and it has been suggested that the spontaneous rejection of MHC-incompatible lymphohematopoietic cells is in fact mediated by NK cells. If the MHC antigens themselves serve as targets in this kind of allorejection, this hypothesis is in apparent contrast with the prevailing view that recognition by NK cells is not guided by, or directed against, MHC-antigens on the target cell surface. Aging athymic nude mice and rats generate cells that rearrange and express T cell receptor (TCR) genes, and this has raised the possibility that T cells or T-like cells in athymic nude animals are responsible for ALC and ABC. This possibility urged the device of an in vitro test system for identification and further characterization of the effector cells in these rejection phenomena. Under appropriate conditions, cells from the rnu rat spleen or liver with natural killer function, i.e. the ability to lyse certain kinds of tumor cells in vitro, are also spontaneously cytotoxic for allogeneic small lymphocytes and bone marrow cells in vitro. Furthermore, these cells can be grown in vitro in the presence of interleukin 2 (IL-2) to generate populations of lymphokine-activated killer (LAK) cells, and these cells have the same spectrum of alloreactivity in ALC and ABC as the native NK cells.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Introduction To the Concept Of Auogeneic Lymphocyte Cytotoxisupporting

confidence: 77%

Non-adaptive cellular immune responses as studied in euthymic and athymic nude rats

Rolstad

Fossum

1990

Anat Embryol

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“…This label has been used previously to establish the nature of short-term lymphocyte migration patterns through the liver (30) In these experiments, no detectable alteration was observed in the short-term migratory characteristics of labeled lymphocytes. Cells damaged during labeling, as is observed with other tracking dyes, do not recirculate (32). However, appropriately labeled cells will remain easily distinguishable and recirculate for long periods of time.…”

Section: Discussionmentioning

confidence: 99%

Rapid turnover of the recirculating lymphocyte pool in vivo

Young

Hay

1995

International Immunology

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“…By simultaneously injecting a small number of labeled lymphocytes together with a variable number of unlabeled lymphocytes, it has been shown that the distribution of labeled lymphocytes is not altered, although up to 10 9 lymphocytes are co-injected (Heslop and Hardy, 1971). In our experience, satisfactory results are obtained by injecting approximately 5 ϫ 10 7 T cells in 2 ml of saline over 2 minutes intravenously into a 200-g rat.…”

Section: Injectionmentioning

confidence: 76%

Analyzing the Migration of Labeled T Cells In Vivo: An Essential Approach with Challenging Features

Westermann

Söllner

Ehlers

et al. 2003

Laboratory Investigation

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SUMMARY:T cells are involved in the pathogenesis of many diseases. To exert a pathological effect, T cells enter the tissues.We show that the determination of their entry site requires isolation of the respective T cell population, injection into genetically un-manipulated animals, and identification of the cells in vivo at various time points after injection. We indicate variables influencing in vivo migration experiments artificially, and outline how resulting problems can be either avoided or taken into account. Reviewing experiments performed according to the outlined criteria reveals two types of migration patterns for T cell subsets in vivo: 1) Naïve and memory T cells enter lymphoid and non-lymphoid organs in comparable numbers, but selectively accumulate in lymphoid tissues over time, 2) Effector T cells, too, enter lymphoid and non-lymphoid organs in comparable numbers. However, most of them die within 24 hours. Depending on the presence of cytokines, chemokines and extracellular matrix compounds they are able to survive, thereby preferentially accumulating in their target tissues. This information might help to understand the role of migration in the pathogenesis of T cell mediated diseases. (Lab Invest 2003, 83:459 -469).

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The Distribution of 51cr-Labeled Syngeneic and Allogeneic Lymph Node Cells in the Rat

Cited by 45 publications

References 0 publications

Non-adaptive cellular immune responses as studied in euthymic and athymic nude rats

Non-adaptive cellular immune responses as studied in euthymic and athymic nude rats

Rapid turnover of the recirculating lymphocyte pool in vivo

Analyzing the Migration of Labeled T Cells In Vivo: An Essential Approach with Challenging Features

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