The distribution coefficients of phenol and substituted phenols in the ammonium sulfate-poly-N-vinylpyrrolidone-water system

Чурилина, Е. В.; Sukhanov, P. T.; Korenman, Ya. I.; Il’in, A. N.; Shatalov, G. V.; Bolotov, V. M.

doi:10.1134/s0036024411040078

Cited by 5 publications

(1 citation statement)

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“…The DNA-PVP binding phenomenon is caused either by hydrogen bonding between PVP and the major groove of DNA (pH 4 to 6) or through ð stacking interactions between aromatic rings [6]. From previous research where crosslinked PVP has been used to extract caffeine, tannins, phenols, indoleacetic acid, DNA and other aromatic compounds, ð- stacking is the likely mode of interaction [7]. For protein, amino acids containing aromatic rings are generally located in the protein’s globular interior and inaccessible, allowing PVP to binds to DNA with high affinity but not bind proteins [8; 9].…”

Section: Introductionmentioning

confidence: 99%

T3: Targeted proteomics of DNA-binding proteins

Nagore

Jarrett

2015

Analytical Biochemistry

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A technique that allows the inclusion of a specific DNA to enrich and direct proteomic identification of transcription factors (TF) while providing a route for high throughput screening on a single platform would be valuable in investigations of gene expression and regulation. Polyvinylpyrrolidone binds DNA avidly while binding negligible amounts of protein. This observation is used in a proof-of-concept method to enrich for TF by combining nuclear extract with a specific DNA sequence and immobilizing the DNA-protein complex on a PVP-coated MALDI plate. Any unbound proteins are washed away and further processed for analysis in a MALDI-TOF/TOF mass spectrometer. Enrichment on a PVP-coated plate gives the unique advantage of purification, enzymatic digestion and analysis on a single platform. The method is termed T3 as it combines Targeted purification on a Target plate with Targeted proteomics. Validation was achieved in model experiments with a chimeric fusion protein, green fluorescent protein-CAAT enhancer binding protein (GFP-C/EBP) with an oligonucleotide containing the CAAT sequence. Both domains were identified with an expectation value of less than 10−15 and over 15% sequence coverage. The same oligonucleotide mixed with HEK293 cell nuclear extract allowed the unambiguous identification of native human C/EBP alpha with 24.3% sequence coverage.

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Section: Introductionmentioning

confidence: 99%

T3: Targeted proteomics of DNA-binding proteins

Nagore

Jarrett

2015

Analytical Biochemistry

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Physicochemical, Solution, and Colloidal Properties of Pyrrolidone and Caprolactam‐Based Polymers

McMullen

Clark

Ozkan

et al. 2021

Handbook of Pyrrolidone and Caprolactam Based Materials

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Poly(vinylpyrrolidone) (PVP) is one of the most widely used polymers for numerous biomedical, pharmaceutical, personal care, and industrial applications. This chapter begins with an introduction to the solution properties of PVP as manifested in a number of different important parameters to describe the physicochemical properties of the polymer in aqueous solutions. One of the key features of PVP is its good solubility in both water and organic solvents. The complexation of PVP with polyphenols is the basis for a variety of applications. The generation of binding isotherms provides insight into the interactions between polymers and cosolutes. There is an increasing interest in interpolymer complexes or polymer‐polymer complexes formed by mixing two or more interacting polymers in solution, since they are used to generate multilayered soft nanomaterials, such as self‐assembled films or nanoparticles. There has been significant interest in subcritical and supercritical fluid technology as substitutes for organic solvents.

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