The distribution and frequency of <i>Fusobacterium nucleatum</i> subspecies in the human oral cavity

Gharbia, SE; Shah, Haroun N.; Lawson, Paul A.; Haapasalo, Markus

doi:10.1111/j.1399-302x.1990.tb00434.x

Cited by 57 publications

(52 citation statements)

References 20 publications

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“…As suggested previously by Gharbia et al (1990), F. nucleatum subsp. polymorphum is more commonly observed in periodontally healthy sites, whilst F. nucleatum subsp.…”

Section: Discussionsupporting

confidence: 57%

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Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S–23S rRNA gene intergenic transcribed spacer region

Gonzales-Marin

Spratt

Allaker

2013

Journal of Medical Microbiology

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“…As suggested previously by Gharbia et al (1990), F. nucleatum subsp. polymorphum is more commonly observed in periodontally healthy sites, whilst F. nucleatum subsp.…”

Section: Discussionsupporting

confidence: 57%

“…nucleatum was more prevalent in diseased sites, whereas isolates from healthy sites were generally identified as F. nucleatum subspp. polymorphum and fusiforme (Gharbia et al, 1990). However, another study did not detect any difference between healthy and diseased sites (Berres et al, 2003).…”

mentioning

confidence: 94%

Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S–23S rRNA gene intergenic transcribed spacer region

Gonzales-Marin

Spratt

Allaker

2013

Journal of Medical Microbiology

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“…vincentii is genetically similar to F. nucleatum subsp. fusiforme (3,13,14,34). Considering these findings, these two subspecies can be classified into a single subspecies.…”

Section: Vol 48 2010 Discrimination Of F Nucleatum Subspecies 549mentioning

confidence: 99%

“…polymorphum : 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 Escherichia coli G T A A C A G G A A G A A G C T T G C T T C C2 G A A T T T G G G T T T T T T A A C C3 G A A T T T G G G T T T T T A A C C4 G A A T T T G G G T T T T T A A C C1 G A A C C5 G A A C C6 G A (13). The difference in the isolation frequency of the subspecies of F. nucleatum from these studies may result from either host foods being consumed in various geographical ethnic groups, the detection methods for the subspecies, or the small sampling sizes employed.…”

Section: Vol 48 2010 Discrimination Of F Nucleatum Subspecies 549mentioning

confidence: 99%

Application of rpoB and Zinc Protease Gene for Use in Molecular Discrimination of Fusobacterium nucleatum Subspecies

Kim¹,

Lee

Chang

et al. 2010

J Clin Microbiol

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Fusobacterium nucleatum is classified into five subspecies that inhabit the human oral cavity (F. nucleatum subsp. nucleatum, F. nucleatum subsp. polymorphum, F. nucleatum subsp. fusiforme, F. nucleatum subsp. vincentii, and F. nucleatum subsp. animalis) based on several phenotypic characteristics and DNA-DNA hybridization patterns. However, the methods for detecting or discriminating the clinical isolates of F. nucleatum at the subspecies levels are laborious, expensive, and time-consuming. Therefore, in this study, the nucleotide sequences of the RNA polymerase ␤-subunit gene (rpoB) and zinc protease gene were analyzed to discriminate the subspecies of F. nucleatum. The partial sequences of rpoB (approximately 2,419 bp), the zinc protease gene (878 bp), and 16S rRNA genes (approximately 1,500 bp) of the type strains of five subspecies, 28 clinical isolates of F. nucleatum, and 10 strains of F. periodonticum (as a control group) were determined and analyzed. The phylogenetic data showed that the rpoB and zinc protease gene sequences clearly delineated the subspecies of F. nucleatum and provided higher resolution than the 16S rRNA gene sequences in this respect. According to the phylogenetic analysis of rpoB and the zinc protease gene, F. nucleatum subsp. vincentii and F. nucleatum subsp. fusiforme might be classified into a single subspecies. Five clinical isolates could be delineated as a new subspecies of F. nucleatum. The results suggest that rpoB and the zinc protease gene are efficient targets for the discrimination and taxonomic analysis of the subspecies of F. nucleatum.

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“…nucleatum is isolated mostly in periodontal diseased sites, whereas F. nucleatum subsp. fusiforme/vincentii is often isolated from healthy sites as normal flora (9). F. nucleatum subsp.…”

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confidence: 99%

Fusobacterium nucleatum Subspecies Identification by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2015

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hWe explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level. Fusobacterium nucleatum is an opportunistic pathogen, associated with various forms of periodontal diseases and extraoral infections, as well as colorectal cancer (1, 2). F. nucleatum is a highly heterogeneous species and was classified into five subspecies: animalis, nucleatum, polymorphum, vincentii, and fusiforme (3-5). Recent studies based on phylogenetic analysis of the nucleic acid sequences of 16S rRNA, rpoB, zinc protease, and 22 other housekeeping genes suggested that F. nucleatum subsp. fusiforme and vincentii be classified into a single subspecies, F. nucleatum subsp. fusiforme/vincentii (4, 5).Different subspecies may vary in pathogenesis relating to different levels of disease activity (6-8). Fusobacterium nucleatum subsp. nucleatum is isolated mostly in periodontal diseased sites, whereas F. nucleatum subsp. fusiforme/vincentii is often isolated from healthy sites as normal flora (9). F. nucleatum subsp. animalis and polymorphum are associated with pregnancy complications (7), and F. nucleatum subsp. animalis is also associated with inflammatory bowel disease (10). Until now, molecular technologies have been the most effective and widely accepted tools for subspecies identification (3,4,11). At the subspecies level of F. nucleatum, the sequence divergences of 16S rRNA genes were only 0.6% to 1.9%, so full-length sequencing of 16S rRNA was desirable (4).This study aimed to explore the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the identification of F. nucleatum at the subspecies level. A commercially available database was amended using 15 F. nucleatum isolates comprising the type strains and other wellcharacterized clinical isolates of the five subspecies and then tested for rapid identification of F. nucleatum subspecies.(This study was presented in part at the 113th (10). A total of 34 F. nucleatum clinical isolates were collected from Case Western Reserve University, Memorial Sloan-Kettering Cancer Center, Harvard University School of Public Health, and bioMérieux, Inc. The isolates were stored in Trypticase soy broth with glycerol at Ϫ80°C, cultivated on Columbia agar (Becton, Dickinson and Company, Sparks, MD) supplemented with 5% sheep blood, and incubated at 37°C for 48 h in an anaerobic chamber, as previously described (10).Full-length 16S rRNA gene sequencing. Bacterial DNA was extracted from a pure colony with a Pure Link genomic DNA minikit (Invitrogen, Carlsbad, CA). The 16S ...

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The distribution and frequency of Fusobacterium nucleatum subspecies in the human oral cavity

Cited by 57 publications

References 20 publications

Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S–23S rRNA gene intergenic transcribed spacer region

Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S–23S rRNA gene intergenic transcribed spacer region

Application of rpoB and Zinc Protease Gene for Use in Molecular Discrimination of Fusobacterium nucleatum Subspecies

Fusobacterium nucleatum Subspecies Identification by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

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