The distinguishing electrical properties of cancer cells

Gregorio, Elisabetta Di; Israel, Simone; Staelens, Michael; Tankel, Gabriella; Shankar, Karthik; Tuszyński, Jack A.

doi:10.1016/j.plrev.2022.09.003

Cited by 16 publications

(3 citation statements)

References 514 publications

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“…However, current research in this eld does not clearly detail the exact mechanisms by which electromagnetic elds interact and impair cancer cell physiology. Other studies have referred to the different electrical characteristics of cancer cells, considering the electronegativity of some of their structures 9,35 , but to date no scienti c evidence support the molecular mechanisms that differentiate the electronegativity of a cancer cell from a normal cell. In spite of these uncertainties, this eld is currently being widely explored for potential therapies.…”

Section: Discussionmentioning

confidence: 99%

“…Major features of the modulator equipment are further explained in the Materials and Methods section, being a new device that discriminates the overall electronegative charge of cell cultures. As cancer cell metabolism may impose drastic differences in their electronegativity 9 , it was of paramount interest to explore whether the modulator equipment can differently impact cells at distinct tumorigenicity stages.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Molecular Modulation by Multifrequency Electromagnetic Pulses to Preferably Eradicate Tumorigenic cells

Piredda,

Martínez,

Stakamakis

et al. 2024

Preprint

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Physics methods of cancer therapy are extensively used in the clinical practice, but they are invasive and often confront undesired side effects. A fully new equipment that allows sustained emission of intense and time controlled non-ionizing multifrequency electromagnetic pulse (MEMP), has been applied to eukaryotic cells in culture. The equipment discriminates the overall electronegative charge of the cell cultures, and its subsequent proportional emission may thereby become higher and lethal to cancer cells of generally high metabolic activity, whereas low tumorigenic cells would be much less affected. We tested the specificity and efficacy of the equipment against a collection of (i) highly tumorigenic cells of human (glioblastoma, cervical carcinoma, and skin) and mouse (colon adenocarcinoma) origin; (ii) cell lines of much lower tumorigenicity (non-human primate kidney and mouse fibroblasts), and (iii) primary porcine macrophages lacking tumorigenicity. Time and intensity control of the MEMP allowed progressive decay of viability fairly correlating to cell tumorigenicity, which was provoked by a proportional alteration of the cytoplasmic membrane permeability, cell cycle arrest at G2, and general collapse of the actin and intermediate filaments cytoskeleton to the perinuclear region. Correspondingly, these effects drastically inhibited the proliferative capacity of the most tumorigenic cells in clonogenic assays. Moreover, MEMP suppressed in a dose-dependent manner the tumorigenicity of retrovirally transduced luciferase expressing colon adenocarcinoma cells in xenografted immune-competent mice, as determined by tumor growth in a bioluminescence imaging system. Our results support MEMP as an anti-cancer non-invasive physical treatment of substantial specificity for tumorigenic cells with promising therapeutic potential in oncology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of Molecular Modulation by Multifrequency Electromagnetic Pulses to Preferably Eradicate Tumorigenic cells

Piredda,

Martínez,

Stakamakis

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…By increasing membrane permeability, electromagnetic fields alter the electrical properties of cells, allowing more ions to enter. This results in changes to membrane potential and cytoplasmic pH, which can trigger apoptosis through various pathways [ 71 ].…”

Section: The Role Of Ion Channels and Membrane Potentials In Cancermentioning

confidence: 99%