The distinct effects of aspirin on platelet aggregation induced by infectious bacteria

Hannachi, Nadji; Baudoin, Jean-Pierre; Prasanth, Arsha; Habib, Gilbert; Camoin-Jau, Laurence

doi:10.1080/09537104.2019.1704717

Cited by 8 publications

(26 citation statements)

References 46 publications

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“…in which, the authors used indomethacin, another cyclo-oxygenase inhibitor and which showed a significant decrease in platelet aggregation (Arman et al, 2014). This benefit has also been confirmed in our recent study (Hannachi et al, 2019a), and in an animal model of IE (Veloso et al, 2015). In our experiments here, we focused on evaluating the effect of aspirin on platelet cyclooxygenase inhibition.…”

Section: Discussionsupporting

confidence: 56%

“…Bacteria concentrations have been previously optimized. Indeed, S. aureus strains were added from initial suspension of 10 9 CFU/ml (Arman et al, 2014;Hannachi et al, 2019a) while S. sanguinis strains were added from initial suspension of 3 • 10 9 CFU/ml to reach a final bacterial concentration in PRP equivalent to 10 8 CFU/ml and 3 • 10 8 CFU/ml respectively. The reaction had proceeded for at least 20 min, and the degree of aggregation was expressed as a percentage of aggregation (Light transmission before the addition of bacterialight transmission after the addition of bacteria) x 100.…”

Section: Light Transmission Aggregometry (Lta)mentioning

confidence: 99%

“…We have reported in a recent study carried out on aspirin that the latter showed distinct effects on platelet aggregation according to the bacterial species involved (Hannachi et al, 2019a). In this current study, we aimed to evaluate all types of oral antiplatelet drugs used currently in clinical practice on platelet aggregation induced by different strains of each bacterial species (S. aureus and S. sanguinis), looking for possible inter species and inter-strain variability.…”

Section: Introductionmentioning

confidence: 98%

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Antiplatelet Agents Have a Distinct Efficacy on Platelet Aggregation Induced by Infectious Bacteria

Hannachi¹,

Ogé-Ganaye

Baudoin³

et al. 2020

Front. Pharmacol.

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Platelets are the cornerstone of hemostasis. However, their exaggerated aggregation induces deleterious consequences. In several diseases, such as infectious endocarditis and sepsis, the interaction between platelets and bacteria leads to platelet aggregation. Despite platelet involvement, no antiplatelet therapy is currently recommended in these infectious diseases. We aimed here, to evaluate, in vitro, the effect of antiplatelet drugs on platelet aggregation induced by two of the bacterial pathogens most involved in infectious endocarditis, Staphylococcus aureus and Streptococcus sanguinis. Blood samples were collected from healthy donors (n = 43). Treated platelet rich plasmas were incubated with three bacterial strains of each species tested. Platelet aggregation was evaluated by Light Transmission Aggregometry. CD62P surface exposure was evaluated by flow cytometry. Aggregate organizations were analyzed by scanning electron microscopy. All the strains tested induced a strong platelet aggregation. Antiplatelet drugs showed distinct effects depending on the bacterial species involved with different magnitude between strains of the same species. Ticagrelor exhibited the highest inhibitory effect on platelet activation (p <0.001) and aggregation (p <0.01) induced by S. aureus. In the case of S. sanguinis, platelet activation and aggregation were better inhibited using the combination of both aspirin and ticagrelor (p <0.05 and p <0.001 respectively). Aggregates ultrastructure and effect of antiplatelet drugs observed by scanning electron microscopy depended on the species involved. Our results highlighted that the effect of antiplatelet drugs depended on the bacterial species involved. We might recommend therefore to consider the germ involved before introduction of an optimal antiplatelet therapy.

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Section: Discussionsupporting

confidence: 56%

Section: Light Transmission Aggregometry (Lta)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Antiplatelet Agents Have a Distinct Efficacy on Platelet Aggregation Induced by Infectious Bacteria

Hannachi¹,

Ogé-Ganaye

Baudoin³

et al. 2020

Front. Pharmacol.

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“…First, the influence of the bacterial species on the ultrastructure of platelet aggregates has been previously demonstrated in vitro, by light and electron microscopy [ 9 ]. E. faecalis induced loose aggregates, with platelets that fully retained their cellular integrity while S. sanguinis and S. aureus were associated with very dense aggregates and coalesced platelets.…”

Section: Discussionmentioning

confidence: 99%

“…If the results of these studies were generally discordant and/or inconclusive, this might indicate that differences in the development process of IE are specific to the patient and the pathogen involved. In fact, we now know that bacterial species act with blood cells and particularly with platelets, in a different way from one another [ 8 , 9 ]. Thus, we hypothesized that the development of vegetation during IE may also depend on the bacterial species involved and the comorbid conditions specific to each patient.…”

Section: Introductionmentioning

confidence: 99%

A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation

Hannachi

Lépidi

Fontanini

et al. 2020

Cells

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Infectious endocarditis (IE) remains one of the deadliest heart diseases with a high death rate, generally following thrombo-embolic events. Today, therapy is based on surgery and antibiotic therapy. When thromboembolic complications in IE patients persist, this is often due to our lack of knowledge regarding the pathophysiological development and organization of cells in the vegetation, most notably the primordial role of platelets and further triggered hemostasis, which is related to the diversity of infectious microorganisms involved. Our objective was to study the organization of IE vegetations due to different bacteria species in order to understand the related pathophysiological mechanism of vegetation development. We present an approach for ultrastructural analysis of whole-infected heart valve tissue based on scanning electron microscopy and energy-dispersive X-ray spectroscopy. Our approach allowed us to detect differences in cell organization between the analyzed vegetations and revealed a distinct chemical feature in viridans Streptococci ones. Our results illustrate the benefits that such an approach may bring for guiding therapy, considering the germ involved for each IE patient.

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Does aspirin have an effect on risk of death in patients with COVID-19? A meta-analysis

Sun

et al. 2022

Eur J Clin Pharmacol

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Purpose The coronavirus disease 2019 (COVID-19) pandemic has shown unprecedented impact world-wide since the eruption in late 2019. Importantly, emerging reports suggest an increased risk of thromboembolism development in patients with COVID-19. Meanwhile, it is found that aspirin reduced mortality in critically ill patients with non-COVID-19 acute respiratory distress syndrome. Therefore, a meta-analysis was performed to investigate the effects of aspirin on COVID-19 mortality. Methods A systematic literature search was conducted in 10 electronic databases and 4 registries. Random effects models were used to calculate pooled relative risks (RRs) with 95% confidence intervals (Cis) to estimate the effect of aspirin on COVID-19 mortality. Relevant subgroup analyses and sensitivity analyses were also performed. Results The results showed that aspirin use was associated with a reduction in COVID-19 mortality (adjusted RR 0.69; 95% CI 0.50–0.95; P < 0.001). Subgroup analysis found that the low-dose group was associated with a reduced COVID-19 mortality (adjusted RR 0.64; 95% CI 0.48–0.85; P < 0.01). Aspirin use was associated with reduced COVID-19 mortality in Europe and America (crude RR 0.71; 95% CI 0.52–0.98; P = 0.04), and results from cohort studies suggested that aspirin use was a protective factor for COVID-19 mortality (adjusted RR 0.73; 95% CI 0.52–0.99; P = 0.04). Meanwhile, aspirin use was not associated with bleeding risk (crude RR 1.22; 95% CI 0.80–1.87; P = 0.96). Conclusions This meta-analysis found that aspirin use was associated with a reduction in mortality in patients with COVID-19 and not with an increased risk of bleeding. Supplementary information The online version contains supplementary material available at 10.1007/s00228-022-03356-5.

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The distinct effects of aspirin on platelet aggregation induced by infectious bacteria

Cited by 8 publications

References 46 publications

Antiplatelet Agents Have a Distinct Efficacy on Platelet Aggregation Induced by Infectious Bacteria

Antiplatelet Agents Have a Distinct Efficacy on Platelet Aggregation Induced by Infectious Bacteria

A Novel Approach for Detecting Unique Variations among Infectious Bacterial Species in Endocarditic Cardiac Valve Vegetation

Does aspirin have an effect on risk of death in patients with COVID-19? A meta-analysis

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