The dispersal of cells from human gynecologic specimens: chemical agents.

Escobar, Gabriel J.; Todd, Paul; Sattilaro, Richard F.

doi:10.1177/25.7.19529

Cited by 11 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the moment, the actual mechanism of action of LIS is not fully understood; however, Escobar et al [5] described the biochemical effects of various agents on cytomembranes. According to his explanation, LIS demonstrates a dual mechanism which may involve: (1) a detergent-like activity and (2) active molecules (e.g., Li ÷, I-) which possess very large hydration spheres and are thus able to pull out glycoprotein molecules from surface membranes.…”

Section: Discussionmentioning

confidence: 98%

“…extracting glycoproteins from the surface of cells as described by Escobar et al [5] and Marchesi et al [16] was utilized to obtain crude soluble antigens from leukemic blast and line 10 ascites tumor cells. Briefly, a Ficoll-Hypaque gradient was employed to isolate the blast cells and the L10 ascites tumor.…”

Section: Extraction Of Soluble Antigens a Modification Of A Techniqumentioning

confidence: 99%

See 1 more Smart Citation

Noncytolytic extraction of soluble antigens from leukemic blast cells (L2C-EN)

Pearson

McIntyre

Garner

et al. 1981

Cancer Immunol Immunother

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Extraction Of Soluble Antigens a Modification Of A Techniqumentioning

confidence: 99%

Noncytolytic extraction of soluble antigens from leukemic blast cells (L2C-EN)

Pearson

McIntyre

Garner

et al. 1981

Cancer Immunol Immunother

View full text Add to dashboard Cite

show abstract

“…Escobar el al. [8] investigated nonenzymatic chemical solutions for dispersal of gynecologic cells and along with others [9] stressed the importance of disulfide bridges in junctional complexes. As demonstrated for other cell types, the corneal endothelial cells are probably held together by surface glycoproteins [19,20,28] and junctional complexes [6,10,18,21].…”

Section: Discussionmentioning

confidence: 99%

An Ultrastructural Study of Retrocorneal Fibrous Membrane-Like Corneal Endothelial Metaplasia In vitro

Kenney¹,

Benya²,

Nimni³

1980

Ophthalmic Res

View full text Add to dashboard Cite

The pathogenesis of many corneal diseases involves alteration in endothelium and subjacent Descemet’s membrane. A common manifestation of these disorders is the appearance of a retrocorneal fibrous membrane (RCM) composed of dissociated endothelial cells interposed between layers of Descemet’s membrane-like materials. The stimulus for the endothelium to lose its epithelioid characteristics and assume this more fibroblastic behavior is unknown. In the present study we describe tissue culture conditions which stimulate isolated corneal endothelial cells to undergo a transition similar to that seen in RCM formation. Accordingly, a monolayer of hexagonal endothelial cells become multilayered metaplastic cells and produce abundant Descemet’s membrane-like material. Cells were isolated by enzyme digestion and modification of thiol groups to yield single or small 2- to 3-cell aggregates which could be evenly dispersed on plastic tissue culture dishes. Contaminating stromal fibroblasts were not present in these preparations as verified morphologically. Cultures were confluent within 5–7 days after plating and at 28 days of incubation, cultures displayed features similar to regenerating rabbit endothelium. These included loss of contact inhibition leading to multilayering of cells, intracytoplasmic densities and extracellular matrix (ECM) which accumulated not only between the basal layer of cells and plastic substratum but also in the cellular interstices. The fibrillar and amorphous components of this ECM accumulated in thin layers and were ultrastructurally similar to the anterior banded and posterior unbanded portions of Descemet’s membrane, respectively. This in vitro lamination of cells and their ECM is highly reminiscent of the RCM accompanying various corneal diseases. Therefore, our in vitro system, leading to the formation of multilaminar corneal endothelial cells, provides an advantageous experimental model for studies of cellular activity leading to RCM formation.

show abstract

Automated slide preparation system for the clinical laboratory

Zahniser¹,

Hurley²

1996

Cytometry

View full text Add to dashboard Cite

An automated device has been developed to prepare a wide variety of cytology preparations. Using the device, cells collected in suspension are mildly dispersed and transferred to a glass slide using filter‐transfer technology. The cell density on the slide is controlled by the instrument. A companion product, the solution used in specimen transport and preparation, provides long‐term preservation of diagnostic cells while lysing red blood cells. The overall process yields clean, uniform samples with better visualization of the diagnostic cells. The use of a disposable filter prevents sample‐to‐sample contamination. Collection of the sample in solution makes it possible to produce multiple slides from one sample. © 1996 Wiley‐Liss, Inc.

show abstract

The dispersal of cells from human gynecologic specimens: chemical agents.

Cited by 11 publications

References 20 publications

Noncytolytic extraction of soluble antigens from leukemic blast cells (L2C-EN)

Noncytolytic extraction of soluble antigens from leukemic blast cells (L2C-EN)

An Ultrastructural Study of Retrocorneal Fibrous Membrane-Like Corneal Endothelial Metaplasia In vitro

Automated slide preparation system for the clinical laboratory

Contact Info

Product

Resources

About