The discovery of proteases and intramembrane proteolysis

Paschkowsky, Sandra; Hsiao, Jacqueline Melissa; Young, Jason C.; Munter, Lisa Marie

doi:10.1139/bcb-2018-0186

Cited by 12 publications

(10 citation statements)

References 82 publications

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“…Regulated intramembrane proteolysis (RIP) is a conserved signal-transducing mechanism that allows information to be transmitted across cellular compartments via a two-step cleavage process [36]. With RIP, EpCAM is first cleaved by metalloprotease tumour necrosis factor-alpha converting enzyme (TACE/ADAM17) close to the extracellular side of the plasma membrane, leading to the shedding of the extracellular domain of EpCAM named EpEX [17].…”

Section: Regulated Intramembrane Proteolysis (Rip)mentioning

confidence: 99%

Revisiting the Roles of Pro-Metastatic EpCAM in Cancer

et al. 2020

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Epithelial cell adhesion molecule (EpCAM) is a cell surface protein that was discovered as a tumour marker of epithelial origins nearly four decades ago. EpCAM is expressed at basal levels in the basolateral membrane of normal epithelial cells. However, EpCAM expression is upregulated in solid epithelial cancers and stem cells. EpCAM can also be found in disseminated tumour cells and circulating tumour cells. Various OMICs studies have demonstrated that EpCAM plays roles in several key biological processes such as cell adhesion, migration, proliferation and differentiation. Additionally, EpCAM can be detected in the bodily fluid of cancer patients suggesting that EpCAM is a pathophysiologically relevant anti-tumour target as well as being utilized as a diagnostic/prognostic agent for a variety of cancers. This review will focus on the structure-features of EpCAM protein and discuss recent evidence on the pathological and physiological roles of EpCAM in modulating cell adhesion and signalling pathways in cancers as well as deliberating the clinical implication of EpCAM as a therapeutic target.

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Section: Regulated Intramembrane Proteolysis (Rip)mentioning

confidence: 99%

Revisiting the Roles of Pro-Metastatic EpCAM in Cancer

et al. 2020

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“…Caspases are a family of cysteine proteases that play an important role in apoptosis as well as its associated biochemical and morphological changes. On the other hand, cytochrome C, which is always present in the mitochondrial intramembrane space, is released into cytosol and, following induction by intermediate metabolits, triggers apoptosis by many chemotherapeutic stimuli and DNA damaging stimuli [13,33,34,35].…”

Section: Discussionmentioning

confidence: 99%

Histopathological Examination of the Effects of Oral Consumption of Various Doses of Propolis in Mice Liver

Samiee-Rad¹,

Bagherkhani²,

Gheibi³

2019

JFNR

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Introduction: Considering the widespread application of biological substances, such as Propolis, it is essential to identify the importance of its liver metabolism, its effects of the liver and safe doses. The aim of this study was to determine the histopathological effects of oral administration of Propolis on the liver in mice. Methods and materials: This study consisted of five experimental groups of male mice (25-30 g), including control, placebo and 3 treatment groups. Male mice (25-30 g). Each of the groups receiving Propolis extract at one of the 2000, 4000 and 8000 mg/kg concentrations. Mice were gavage fed with the alcoholic extract of Iranian Propolis once daily for 14 days. After induction of a deep anesthesia, the liver was removed and examined for histopathological changes. All the data were analyzed using SPSS software (P <0.05). Results: The results of this study indicated that chronic administration of various doses of routine Propolis induced different histopathological changes in a dose-dependent manner. These changes initiated around the central venous; furthermore, their severity was higher in this part of the liver compare to the other areas. Conclusion: Based on the results of this study, it is essential to carefully monitor the dosage and consumption duration of Propolis. Moreover, periodical examination of liver function is essential during chronic consumption of Propolis.

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“…EpCAM signaling requires regulated intramembrane proteolysis (RIP), a conserved signal-transducing mechanism that allows the transit of information across cellular compartments [ 23 ]. EpCAM, as a substrate of RIP, is first cleaved by metalloprotease tumor necrosis factor-alpha converting enzyme (TACE/ADAM17), which leads to the release of EpEX [ 24 ].…”

Section: Epcam In Cancermentioning

confidence: 99%

Epithelial Cell Adhesion Molecule: An Anchor to Isolate Clinically Relevant Circulating Tumor Cells

Eslami-S¹,

Cortés‐Hernández²,

Alix‐Panabières³

2020

Cells

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In the last few decades, the epithelial cell adhesion molecule (EpCAM) has received increased attention as the main membrane marker used in many enrichment technologies to isolate circulating tumor cells (CTCs). Although there has been a great deal of progress in the implementation of EpCAM-based CTC detection technologies in medical settings, several issues continue to limit their clinical utility. The biology of EpCAM and its role are not completely understood but evidence suggests that the expression of this epithelial cell-surface protein is crucial for metastasis-competent CTCs and may not be lost completely during the epithelial-to-mesenchymal transition. In this review, we summarize the most significant advantages and disadvantages of using EpCAM as a marker for CTC enrichment and its potential biological role in the metastatic cascade.

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The discovery of proteases and intramembrane proteolysis

Cited by 12 publications

References 82 publications

Revisiting the Roles of Pro-Metastatic EpCAM in Cancer

Revisiting the Roles of Pro-Metastatic EpCAM in Cancer

Histopathological Examination of the Effects of Oral Consumption of Various Doses of Propolis in Mice Liver

Epithelial Cell Adhesion Molecule: An Anchor to Isolate Clinically Relevant Circulating Tumor Cells

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