The Discovery of Enfumafungin, a Novel Antifungal Compound Produced by an Endophytic Hormonema Species Biological Activity and Taxonomy of the Producing Organisms

Peláez, Fernando; Cabello, Angeles; Platas, Gonzalo; Dı́ez, Maria Teresa; Val, Antonio González del; Basilio, Ángela; Martán, Isabel; Vicente, Francisca; Bills, Gerald F.; Giacobbe, Robert A.; Schwartz, Robert E.; Onishi, Janet C.; Meinz, María; Abruzzo, George K.; Flattery, Amy; Kong, Li; Kurtz, Myra B.

doi:10.1016/s0723-2020(00)80062-4

Cited by 130 publications

(78 citation statements)

References 29 publications

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“…Of the 53 isolates screened, antifungal activity was detected in five strains, but by HPLC analysis only two of them were found to produce 66, in amounts similar to the strain ATCC 74360. 41 …”

Section: Metabolites Of Mevalonate Originmentioning

confidence: 99%

Natural Products from Plant-Associated Microorganisms: Distribution, Structural Diversity, Bioactivity, and Implications of Their Occurrence

2006

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A growing body of evidence suggests that plant-associated microorganisms, especially endophytic and rhizosphere bacteria and fungi, represent a huge and largely untapped resource of natural products with chemical structures that have been optimized by evolution for biological and ecological relevance. A diverse array of bioactive small molecule natural products has been encountered in these microorganisms. The structures of over 230 metabolites isolated and characterized from over 70 plant-associated microbial strains during the past four years are presented with information on their hosts, culture conditions, and biological activities. Some significant biological and ecological implications of their occurrence are also reviewed.

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Section: Metabolites Of Mevalonate Originmentioning

confidence: 99%

Natural Products from Plant-Associated Microorganisms: Distribution, Structural Diversity, Bioactivity, and Implications of Their Occurrence

2006

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“…In previous works, there have been some reports on the antimicrobial (Tanaka, Fukushima, Tsujiro, & Fujimori, 1997;Gong & Guo, 2009;Du, Li, Li, Shang, & Wang, 2012), antioxidant (Huang, Chen, Chang, Sheu, & Lin, 2007;Dhankhar, Kumar, Dhankhar, & Yadav, 2012;Nath, Raghunatha, & Joshi, 2012), anti-inflammatory (Deshmukh et al, 2009;Pretsch et al, 2014) activity of the endophytic fungi. Besides, some in vivo studies on the anti-cancer, anti-diabetic and other bioactivity (Konrath et al, 2012;Govindappa et al, 2015;Tan et al, 2015) have been evidenced, but in vivo antimicrobial studies on endophytic fungi are very scarce (Peláez et al, 2000;Nath & Joshi, 2016).In the present study, we aimed to identify C. gigantea fungal endophytes and to characterize their anti-candidal activity in an in vivo model. …”

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confidence: 91%

Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea

Nath

Joshi

2017

RBT

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Candida albicans is a most common cause of fungal infections in animals and birds. Understanding the increasing resistance of C. albicans to various antifungal therapeutic agents is important to discover new anti-candidal alternatives. The present study investigated the anti-candidal potential of five endophytic fungi extracts, isolated from the tropical ethnoveterinary plant, Calotropis gigantea. We firstly evaluated the in vitro antifungal activities of endophytic fungi extracts by the well diffusion method. Secondly, the cells of C. albicans were treated with the potent extract to observe significant ultrastructural changes. To further investigate the in vivo antifungal activity of the extract, some laboratory experiments with mice were undertaken, and posteriourly, the different organs were studied under the electron microscope for any deformities. Phomopsis asparagi showed the best anti-candidal activity with a minimum inhibitory concentration (MIC) of 46.9 µg/mL. The fungal test pathogen (C. albicans) exhibited various cell deformities when treated with the extract of P. asparagi. Histopathological studies of the vital organs of mice treated with the potent fungal extract did not show any significant pathological conditions when viewed under scanning electron microscope. Thus, P. asparagi can be a potential candidate for anti-candidal agents against C. albicans. Future studies will focus on the isolation of the bioactive components of the extract. Rev. Biol. Trop. 65 (4): 1437-1447. Epub 2017 December 01.Key words: endophytic fungi, anti-candidal, Candida albicans, scanning electron microscope, ultrastructure.Candida albicans is recognized to cause many important diseases in animals and birds, and represents an important veterinary pathogen. C. albicans mostly affects birds causing thrush affecting the oral mucosa, oesophagus and crop. Cattle, calves, sheep and foals are also susceptible to systemic candidiasis which occurs as a consequence of prolonged treatment with antibiotics and corticosteroids (Kuwamura, Ide, Yamate, Shiraishi, & Kotani, 2006). C. albicans infections faces a number of problems including limited number of effective antifungal agents, toxicity of the available antifungal agents, resistance of Candida to commonly used antifungal, relapse of Candida infections and non-cost effective antifungal agents (Sasidharan, Zuraini, Latha, & Suryani, 2008). Therefore, there is an urgent need to design new drugs and to explore alternative sources of novel drugs.Northeast (NE) India is considered one of the most important biodiversity hotspots of the globe with a wide range of physiographic and ecoclimatic conditions (Myers, Mittermeier, Mittermeier, Fonseca, & Kent, 2000). Northeast India in the Eastern Himalayan range is located between 20°35'37.2624"N -78°57'46.368"E, and sprawling over 262 379 km 2 (Lyngwi, Koijam, Sharma, & Joshi, 2013). In tropical and subtropical countries, with hot and humid climates like India, with ideal conditions for fungal infections, turn them into a common p...

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“…En 1989, se reportó el precursor de caspofungina (MK991) 11 y en 1990 el de micafungina (FK463) 12 . Se han descrito varios otros análogos y derivados de equinocandinas; la enfumafungina, las arbocandinas, las papulacandinas, la pneumocandina B, la arundifungina y la mulucandina [13][14][15][16][17] . Otras como la cilofungina han sido abandonadas en su desarrollo clínico dada su difícil formulación y gran toxicidad.…”

Section: El Génesis De Las Equinocandinasunclassified

Nuevos antifúngicos: Las equinocandinas

2004

Rev. chil. infectol.

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The lipopeptide family known as echinocandins emerge as the new "antifungal penicillins", because their ability to destroy the fungal cell wall as they inhibit glucan synthesis, the main component of fungal structure. Echinocandins are fungicidal in vitro and in vivo against most Candida species and fungistatic against Aspergillus sp, without antifungal activity over mammal cells. Three drugs are representative of this class; caspofungin, micafungin and anidulafungin, the two first have been licensed for human use. Their optimal security profile, with low incidence and severity of adverse effects, kind posology and few interactions with other drugs, represent noticeable advantages for modern antifungal therapy. They have similar clinical efficacy as amphotericin B, without its toxicity, which besides the absence of antagonism with other antifungal drugs, allows to suggest that combined antifungal therapy could represent a new standard for the management of the feared invasive aspergillosis.Key words: Echinocandins; Caspofungin; Micafungin; Anidulafungin. Palabras claves: Equinocandinas; Caspofungina; Micafungina; Anidulafungina. IntroducciónEn las últimas décadas asistimos a la emergencia de los hongos como patógenos oportunistas humanos. Sobre 7% de los pacientes en hospitales universitarios europeos fallecen de aspergilosis y diversas especies de Candida se han constituido en causa común de infección nosocomial 1,2 . Ciertos grupos de pacientes inmunocomprometidos presentan alto riesgo de micosis severas; por ejemplo, 15% de los receptores de trasplante alogeneico de células madres 3 , y casi 20% de los sometidos a trasplantes pulmonares, están colonizados o infectados 4 ; en los pacientes con SIDA de países desarrollados la neumonía por Pneumocystis jiroveci (ex P. carinii) y la candidiasis esofágica alcanzan incidencias de 60 y 20% respectivamente 5 . En Chile, Lucero y cols reportan 9,2% de infecciones fúngicas profundas en niños con episodios de neutropenia febril 6 ; por otro lado, Wolff y cols 7 reportan que en el seguimiento de una cohorte de 166 pacientes adultos con infección por VIH y sin terapia antiretroviral, 17,2% por año presentó candidiasis esofágica, 9,1% por año neumonía por P. jiroveci y 4,1% por año criptococosis meníngea.Diversos factores dan cuenta de este aumento en las infecciones fúngicas, a saber, el mejor manejo de otras complicaciones de la inmunodepresión, nuevos y cada vez más agresivos regímenes inmunosupresores, mayor sobrevida en pacientes de unidades de cuidados críticos, alta incidencia de procedimientos invasores y cateterizaciones, mayor sospecha clínica, mejores métodos diagnósticos y el uso exagerado de antimicrobianos.A la fecha, el manejo de las infecciones micóticas sistémicas se sostiene en tres grupos de antifúngicos; polienos (anfotericina B), azoles (itraconazol, fluconazol, voriconazol, entre otros) y la fluocitosina (no disponible en Chile) (Tabla 1). A pesar del importante aporte de estos fármacos al manejo de las infecciones fúngicas, los índices de fracaso ele...

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The Discovery of Enfumafungin, a Novel Antifungal Compound Produced by an Endophytic Hormonema Species Biological Activity and Taxonomy of the Producing Organisms

Cited by 130 publications

References 29 publications

Natural Products from Plant-Associated Microorganisms: Distribution, Structural Diversity, Bioactivity, and Implications of Their Occurrence

Natural Products from Plant-Associated Microorganisms: Distribution, Structural Diversity, Bioactivity, and Implications of Their Occurrence

Anti-candidal effect of endophytic fungi isolated from Calotropis gigantea

Nuevos antifúngicos: Las equinocandinas

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