The Discovery of Appalachia

Inscoe, John C.

doi:10.1002/9780470996300.ch22

Cited by 2 publications

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References 3 publications

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“…This "microsomal" enzyme as compared with the soluble catechol-0-methyltransferase, showed only about loo/, of the specific activity and only about 4O/, of the total activity. Similar results have been reported by Inscoe et al [16], using different subcellular fractions of rat and rabbit liver; they found that the total actvity of the catechol-0-methyltransferase in the microsomal fraction was only about 1 ,Ilo of that in the soluble fraction.…”

Section: Discussionsupporting

confidence: 78%

Purification and Properties of a Catechol‐O‐Methyltransferase of Human Liver

Ball

Knüppen

Breuer

1971

European Journal of Biochemistry

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An X-adenosylmethionine : catechol-0-methyltransferase occurs in the 105000 x g supernatant of human liver. As compared with the homogenate, the enzyme was purified 380-fold by adjusting the 24000xg supernatant to pH 5 , fractionation with (NH,),SO,, gel filtration on Sephadex G-200 and G-100 columns and ion-exchange chromatography on CM-Sephadex and DEAEcellulose columns. The purified enzyme preparation was homogeneous when subjected to further chromatography on Sephadex G-100 and DEAE-cellulose, disc electrophoresis on polyacrylamide gel a t pH 9.5 and analytical ultracentrifugation. The enzyme exhibited a ~2 0 ,~ of 3.6 S with a molecular weight of 29000. The final preparation of catechol-0-methyltransferase was very labile, but could be stabilised by the addition of EDTA and MgC1, in equimolar concentrations. The activity of the enzyme was assayed in the presence of cysteine and MgC1, with 2-hydroxyoestradiol-17p and radioactive X-adenosylmethionine as substrates. The metabolites of 2-hydroxyoestradiol-I 78 formed during incubation were identical with 2-methoxyoestradiol-178and 2-hydroxyoestradiol-17,3 3-methyl ether. Throughout the purification procedure, the ratio of the 2-and 3-methyl ethers, respectively, remained constant a t 2.1.I n 1958 Axelrod and Tomchick [I] described an enzyme system from rat liver which catalyses the transfer of the methyl group from X-adenosylmethionine to one of the phenolic hydroxyl groups of epinephrine and other catechols. The enzyme was also found in liver tissue of other mammalian species, including man [I]. Most of the attempts to purify and characterise the catechol-0-methyitransferase have been limited to rat liver [I-41. This paper describes the purification and some properties of a soluble catechol-0-methyltransferase from human liver. 2-Hydroxyoestradiol-178 was used as substrate, as previous studies [5-71 have shown that catechol oestrogens are methylated by the enzyme to a even greater extent than catecholamines. MATERIALS AND METHODS Materials2-Hydroxyoestradiol-l7/?, i.e. 1,3,5(10)-oestratriene-2,3,17/3-triol, was generously donated by Schering (Berlin, Germany). 2-Methoxyoestradiol-l7/?, i.e. 2-methoxy-1,3,5(10)-oestratriene-3,17~-diol, and Enzyme. S-Adenosylmethionine :catechol-0-methyltransferase (EC 2.1.1.6).Trivial Names. 2-Hydroxyoestradiol-17j3, 1,3,5(10)-oestratriene-2,3,17B-triol; 2-methoxyoestradiol-l7~, 2-methoxy-1,3,5(10)-oestratriene-3,17~-diol; 2-hydroxyoestradiol-178 3-methyl ether, 3-methoxy-1,3,5(10)-oestratriene-2,17j3-diol.

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Section: Discussionsupporting

confidence: 78%

Purification and Properties of a Catechol‐O‐Methyltransferase of Human Liver

Ball

Knüppen

Breuer

1971

European Journal of Biochemistry

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“Far Back in American Time”: Culture, Region, Nation, Appalachia, and the Geography of Voice

Revill

Gold

2018

Annals of the American Association of Geographers

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This paper develops a geography of voice in order to address the ways in which cultures, regions and nations are imagined, figured and defined. It adopts Connor's (2000) notion of 'vocalic space' as a starting point from which to explore folk song collecting practices in Appalachia. It develops this in relation to Bauman and Briggs (2003) post-colonial critique of the status of language and speech in ethnographic theory. Historically the Appalachian region has received substantial ethnographic cultural study. Working with insights supplied by collecting activities and subsequent writings of two key collectors-Cecil Sharp (1859-1924) and Alan Lomax (1915-2002)-this paper offers a sociomaterial conception of voice key to its affective politics, whilst examining historical theorisations. These are firstly, derived from folklore and ethnography, later anthropology and sociology and secondly, articulated with regard to geographies of region and nation. These are then considered in relation to geographer James Duncan's (1980, 1998) critique of the 'superorganic' as an explanation of regional cultural distinctiveness. It concludes by arguing that a geography of voice can contribute to critical approaches to regionalism. An understanding of how vocalic spaces are figured and assembled is key to explaining how culture can be translated through levels of abstraction in ways which can marginalise and disenfranchise the very peoples who are the subject of study.

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The Discovery of Appalachia

Cited by 2 publications

References 3 publications

Purification and Properties of a Catechol‐O‐Methyltransferase of Human Liver

Purification and Properties of a Catechol‐O‐Methyltransferase of Human Liver

“Far Back in American Time”: Culture, Region, Nation, Appalachia, and the Geography of Voice

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