2017
DOI: 10.1016/j.bcp.2017.01.016
|View full text |Cite
|
Sign up to set email alerts
|

The discovery, complex crystal structure, and recognition mechanism of a novel natural PDE4 inhibitor from Selaginella pulvinata

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
29
0

Year Published

2020
2020
2024
2024

Publication Types

Select...
8

Relationship

1
7

Authors

Journals

citations
Cited by 43 publications
(32 citation statements)
references
References 39 publications
0
29
0
Order By: Relevance
“…Selaginellin; selaginellins D, F, O, P, Q, S, and W; selaginpulvilins A, B, E, F, I, K, L, N, O, and T; with selariscins A, and B; and selariscinins B, and D were the unique compounds identified from both the DCM and methanolic (MeOH) extracts. Selaginellin was initially isolated from S. sinensis [31], selaginellin S from S. moellendorffii Hieron [40]; selaginellin O [33], selaginpulvilin N [36], selaginpulvilin O [36], selaginpulvilin T [36], selariscin A [36], selariscin B [36], selariscinin B [3], and selariscinin D [3] from S. tamariscina; and selaginellin D [29], selaginellin F [29], selaginellin P [30], selaginellin Q [30], selaginpulvilin A [41], selaginpulvilin B [41], selaginpulvilin E [42,43], selaginpulvilin F [43], selaginpulvilin I [43], selaginpulvilin K [44], and selaginpulvilin L [44] were isolated for the first time from S. pulvinata.…”
Section: Discussionmentioning
confidence: 99%
“…Selaginellin; selaginellins D, F, O, P, Q, S, and W; selaginpulvilins A, B, E, F, I, K, L, N, O, and T; with selariscins A, and B; and selariscinins B, and D were the unique compounds identified from both the DCM and methanolic (MeOH) extracts. Selaginellin was initially isolated from S. sinensis [31], selaginellin S from S. moellendorffii Hieron [40]; selaginellin O [33], selaginpulvilin N [36], selaginpulvilin O [36], selaginpulvilin T [36], selariscin A [36], selariscin B [36], selariscinin B [3], and selariscinin D [3] from S. tamariscina; and selaginellin D [29], selaginellin F [29], selaginellin P [30], selaginellin Q [30], selaginpulvilin A [41], selaginpulvilin B [41], selaginpulvilin E [42,43], selaginpulvilin F [43], selaginpulvilin I [43], selaginpulvilin K [44], and selaginpulvilin L [44] were isolated for the first time from S. pulvinata.…”
Section: Discussionmentioning
confidence: 99%
“…Then, were purified by using similar protocols as previously reported. 31,44 Enzymatic Assays. The enzymatic activities of the catalytic domain of PDE10A2 were measured with 3 H-cAMP (20,000−30,000 cpm, GE Healthcare) as the substrate in a buffer composed of 50 mM Tris, pH = 7.5, 4 mM MgCl 2 , and 1 mM dithiothreitol.…”
Section: ■ Methodsmentioning
confidence: 99%
“…The recombinant PDE10A catalytic domain (residues 449–770) was expressed and purified by using previously reported protocols. , Briefly, the plasmid (pET15b-PDE10A) was transferred to E. coli strain BL21 (Codonplus, Stratagene). Then, the E. coli cells carrying the recombinant plasmid were grown in 2XYT medium (containing 100 μg/mL ampicillin and 30 μg/mL chloramphenicol) at 37 °C until an absorption of OD 600 = 0.6–0.8.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…S. pulvinata is used in traditional Chinese medicine for the treatment of traumatic injury and asthma (Cao et al, 2010). Pharmacological investigations revealed its various biological activities, such as anti-cancer (Wang et al, 2016) and anti-inflammatory effects (Huang et al, 2017). Unfortunately, because of its overexploitation, populations of S. pulvinata have decreased sharply.…”
mentioning
confidence: 99%