The direction of selection of genetically heterogeneous cell populations ofDioscorea deltoidea Wall

Karanova, S. L.; Shamina, Z. B.

doi:10.1007/bf02923266

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…In the 2020s, however, the strain was successfully adopted by a new commercial company currently producing a series of cosmetics and food additives on its base (https://cosmevita.ru/collections/, accessed on 24 January 2023). Small-aggregated, rapidly growing cell strains developed through mutagenesis (single and double treatment with N-nitroso-N-methylurea) [77]; super-producer of steroidal glycosides protodioscin and deltoside and their 25(S)-isomers [60,78,79], adapted for large-scale bioreactor cultivation [80,81]. The total content of steroidal glycosides 4.6-5.7% DW [79] can be increased up to 13.9% DW in bioreactor production with a high aeration level [60,81].…”

Section: Biotechnological Application Of Plant Cell Strains From the ...mentioning

confidence: 99%

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

Yuorieva,

Sinetova,

Messineva

et al. 2023

Biology

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Ex situ collections of algae, cyanobacteria, and plant materials (cell cultures, hairy and adventitious root cultures, shoots, etc.) maintained in vitro or in liquid nitrogen (−196 °C, LN) are valuable sources of strains with unique ecological and biotechnological traits. Such collections play a vital role in bioresource conservation, science, and industry development but are rarely covered in publications. Here, we provide an overview of five genetic collections maintained at the Institute of Plant Physiology of the Russian Academy of Sciences (IPPRAS) since the 1950–1970s using in vitro and cryopreservation approaches. These collections represent different levels of plant organization, from individual cells (cell culture collection) to organs (hairy and adventitious root cultures, shoot apices) to in vitro plants. The total collection holdings comprise more than 430 strains of algae and cyanobacteria, over 200 potato clones, 117 cell cultures, and 50 strains of hairy and adventitious root cultures of medicinal and model plant species. The IPPRAS plant cryobank preserves in LN over 1000 specimens of in vitro cultures and seeds of wild and cultivated plants belonging to 457 species and 74 families. Several algae and plant cell culture strains have been adapted for cultivation in bioreactors from laboratory (5–20-L) to pilot (75-L) to semi-industrial (150–630-L) scale for the production of biomass with high nutritive or pharmacological value. Some of the strains with proven biological activities are currently used to produce cosmetics and food supplements. Here, we provide an overview of the current collections’ composition and major activities, their use in research, biotechnology, and commercial application. We also highlight the most interesting studies performed with collection strains and discuss strategies for the collections’ future development and exploitation in view of current trends in biotechnology and genetic resources conservation.

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