The Direct Estimation of True Blood Sugar.

West, Edward S.; Peterson, Vernon L.; Scharles, Frederick H.

doi:10.3181/00379727-26-4298

Cited by 4 publications

(3 citation statements)

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“…Carbohydrates. All extracts were clarified by the method of West, Scharles and Peterson (26) and sugar was determined in the filtrates by the Munson and Walker method (1, page 190).…”

Section: Nitrate Nitrogenmentioning

confidence: 99%

Chemical investigations of the tobacco plant. II, The chemical changes that occur during the curing of Connecticut shade-grown tobacco

Vickery¹

1931

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Connecticut Experiment Station Bulletin 324 changes were allowed to continue until experience showed that little further alteration was to be expected. The selection of the tobacco leaf for these investigations was made largely as the result of practical considerations. In order that the tissue may be killed before extensive change due to the removal from the plant can occur a large, thin leaf is desirable. The leaves selected should be uniform in size and as free as possible from petioles, buds, or structures other than leaf blade and vascular tissue. If elaborate studies are planned it is also necessary that the leaves should be of uniform genetic origin and chat the plants should be grown under carefully controlled conditions that are to a large extent reproducible from year to year.

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“…Carbohydrates. All extracts were clarified by the method of West, Scharles and Peterson (26) and sugar was determined in the filtrates by the Munson and Walker method (1, page 190).…”

Section: Nitrate Nitrogenmentioning

confidence: 99%

Chemical investigations of the tobacco plant. II, The chemical changes that occur during the curing of Connecticut shade-grown tobacco

Vickery¹

1931

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“…West, Scharles and Peterson's [1929] method for total carbohydrate estimation was employed. The liver tissue after hydrolysis in N H2S04 was treated with mercuric sulphate to precipitate protein, the reducing power of the filtrate estimated by ShafferHartmann reagent and the values so obtained checked by yeast fermentation.…”

Section: Methodsmentioning

confidence: 99%

Glycogenolysis in fish-liver at low temperatures

Sharp¹

1935

Biochemical Journal

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SINCE the time of Claude Bernard it has been known that the end-products of glycogenolysis in muscle and liver are different, but whilst considerable information is now available about this process in muscle, comparatively little is known regarding the detailed mechanism of glycogenolysis in liver. Results obtained from study of the breakdown of glycogen and accumulation of lactic acid in fish-muscle at low temperatures [Sharp, 1934 and preceding paper] made it of additional interest to enquire into the post mortem glycogenolytic changes taking place in fish-liver under similar conditions of freezing and storage.As in the previous work, haddocks which had been lightly hooked on lines set off-shore were put into tanks of circulating sea water and kept there until, after 7-10 days' captivity, they returned to a normal resting condition. When required for experiment the fish were netted quickly, killed by a blow on the back of the head and the livers excised immediately. In order to exclude initial changes due to the struggling of the fish during catching, only fish which had undergone a minimum of struggling were used for experiment. Preliminary work was concerned with the distribution of carbohydrate throughout the tissue, immediately post mortem, and with the development of a reliable technique for estimation of the free sugar fraction. EXPERIMENTAL.Methods.Glycogen concentrations were determined by Pfluiger's method, the reducing values of the final hydrolysates being estimated bytheShaffer-Hartmann reagent. In the tables all glycogen concentrations are given in glucose equivalents.For estimation of lactic acid the tissue was extracted with ice-cold 5 % trichloroacetic acid and the interfering substances were removed from the filtrate by copper-lime precipitation, according to Holmes and Gerard [1929]. The final analyses were carried out by the method of Friedemann et al. [1927].Free sugar was extracted with ice-cold alcohol and the fraction freed from protein by the technique to be described later under method A.Distribution of carbohydrate in liver tissue. Each liver after excision was pressed gently between filter-papers to remove adherent blood and then sliced into 2, 4 or 6 samples. The samples were immediately introduced into tared receptacles containing the necessary extractants. The results, given in Table I, show only slight differences in the distributions of glycogen, free sugar and lactic acid throughout the tissue.

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“…Its presence was questionable, even after incubation under conditions which favour glucose formation. In a series of experiments parallel determinations were made of glycogen and of total carbohydrate by the method of West et al [1929]. Typical data are presented in Table 1.…”

Section: Identification Of the Potysaccharide In Adipose Tissuementioning

confidence: 99%