The Direct Electrochemical Detection of Amino Acids at a Platinum Electrode in an Alkaline Chromatographic Effluent

Polta, John A.; Johnson, Dennis C.

doi:10.1080/01483918308064887

Cited by 92 publications

(32 citation statements)

References 13 publications

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“…Amino acids are usually separated by cation exchange followed by ninhydrin derivatization and photometric detection. Separation of amino acids by anion exchange followed by pulsed electrochemical detection (PED) 2 were first described by Johnson et al in 1983 (2). PED does not require any detection-enabling chemical derivatization.…”

mentioning

confidence: 99%

New Technique for Increasing Retention of Arginine on an Anion-Exchange Column

Jandík¹,

Cheng²,

Jensen³

et al. 2000

Analytical Biochemistry

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mentioning

confidence: 99%

New Technique for Increasing Retention of Arginine on an Anion-Exchange Column

Jandík¹,

Cheng²,

Jensen³

et al. 2000

Analytical Biochemistry

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“…Polta and Johnson were the first to apply PED for the determination of amine compounds, specifically amino acids and amino sugars at a Pt working electrode [68,69].…”

Section: Hplc-ped Of Amine Compounds Many Aliphatic Compounds Inclumentioning

confidence: 99%

“…Adams [1] stated that "...aliphatic amines are difficult to anodically oxidize in any quantitative fashion" at solid metal electrodes, and Malfoy and Reynaud [2] concluded: "Among the 20 amino acids not containing sulfur atoms, present in the proteins, only tryptophan and tyrosine are selectively oxidizable at solid electrodes." It perhaps was not coincidental tliat Malfoy and Reynaud made this comment in a paper studying amino acid oxidation at Au, a noble metal that has no empty d-orbitals to facilitate interaction with the lone-pair electrons of amines and thus promote reactivity.…”

Section: General Introductionmentioning

confidence: 99%

“…Furthermore, they are often utilized for metal surface finishing, gas purification, and as additives and dyes in cleaning solutions [1][2]. Because alkanolamines are used in many ways, and since they have been identified as pollutants in certain waste water effluents [3], there is a strong need to quantify alkanolamines both sensitively and accurately, and without extensive sample manipulation.…”

Section: Introductionmentioning

confidence: 99%

“…Aliphatic amines serve as precursors in dyes and pharmaceuticals, and are used as stabilizers, emulsifiers and corrosion inhibitors in various formulations [1]. In addition, amines are found in biological systems as products of amino acid metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Pulsed electrochemical detection for the investigation and determination of amine compounds separated by high performance liquid chromatography

Dobberpuhl

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Electrooxidation of Aliphatic Amines and Their Amperometric Detection in Flow Injection and Liquid Chromatography at a Nickel-Based Glassy Carbon Electrode

1998

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The electrocatalytic oxidation of mono-and poly-amines was investigated in alkaline solutions at a nickel-based chemically modified glassy carbon electrode. b-NiOOH exhibited electrocatalytic activity towards amines oxidation. FIA experiments were performed to test the electrode as an amperometric sensor. Under constant-potential amperometric mode (at 0.55 V vs. 4,0 M Ag/AgCl/Cl ¹ ), detection limits of all investigated analytes ranged within 1.2-3.8 pmol (S/N = 3), and the linear dynamic range extended over three orders of magnitude above the detection limits. Chromatographic separations, performed by an ion moderated partition column in alkaline solutions equipped with the Ni-CME detector, allowed very simple quantitation of aliphatic amines, avoiding tedious and time-consuming derivatization processes or postcolumn addition procedures. The Ni-CME showed good temporal stability and appreciable sensitivity.

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The Direct Electrochemical Detection of Amino Acids at a Platinum Electrode in an Alkaline Chromatographic Effluent

Cited by 92 publications

References 13 publications

New Technique for Increasing Retention of Arginine on an Anion-Exchange Column

New Technique for Increasing Retention of Arginine on an Anion-Exchange Column

Pulsed electrochemical detection for the investigation and determination of amine compounds separated by high performance liquid chromatography

Electrooxidation of Aliphatic Amines and Their Amperometric Detection in Flow Injection and Liquid Chromatography at a Nickel-Based Glassy Carbon Electrode

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