The Direct Effects of X-Irradiation on the Biosynthesis of Androgens by Rat Testicular Tissue in Vitro

Mechanisms of aging in relationship to steroid biotransformations and the thiobarbituric acid reactive material in rate testicular tissue in vitro

“…This contrasts with the results of the kinetic study performed with _in vitro incubations of adult murine testes utilizing the same radioactive precursors by Ellis and Berliner (1965) and Simpson and Ellis (1967). These authors reported that with a 15-minute incubation period, the amount of tritium exceeded that of carbon-14 in the testosterone.…”

“…In this respect, Simpson and reported differences in the yield of 17a-hydroxyprogesterone and androgens with the time of incubation when a 3 kinetic study was undertaken for the conversion of pregnenolone-7a-H 14 and progesterone-4-C by minced adult murine testes. Noumura, Weiss, and Lloyd (1966) did not observe a significant decrease in the production of testosterone when the incubation time of rat fetal testes homogenates was increases from 30 minuses to 3 hours, but and Nayfeh, Barefoot, and Baggett (1966) did report a decrease in testosterone production and an increase in the formation of androstanediol when the time of incubation was similarly lengthened from 30 minutes to 3 hours.…”

“…If one takes into account the observation that with minced murine testis, incubated for periods longer than 15 minutes with double-isotope precursors, this ratio is usually smaller than one Berliner, 1963, 1965;Simpson and Ellis, 1967). It is possible that the pathways for the production of testosterone at the level of the interstitium and at the level of the seminiferous tubules follow completely different routes after 15 minutes.…”

X-Ray induced changes in biochemical and histochemical parameters in the testis of male wild Indian house rate, <i>Rattus rattus</i>

“…The steroid biotransformations can be altered by irradiation and this was first shown by Rosenfeld et al (1955) and Berliner et al (1962), for the adrenal glands. Testicular steroid biotransformations were shown to be altered by various modes of Xirradiation by Shikita and Tamaoki (1965) and Simpson and Ellis (1967).…”