The direct action of hyaluronic acid on human U-937 and HL-60 cells — modification of native and model membranes

Abstract

The aim of the study was to investigate the effect of application of exogenous hyaluronic acid (HA) of various molecular weights on the cells of human immune system. Two cell lines HL-60 and U-937 with various ability to differentiation were chosen. HL-60 cells were differentiated to macrophages and granulocytes, whereas U-937 only to macrophages. For all investigated cell systems the most cytotoxic effect, indicated as a decrease of cell viability, was found at HA dose equal to 200 mg/L. However, greater effect was observed for differentiated cells and at longer exposure to HA. The possibility of HA interaction with both specific receptors and membrane lipids was tested by determination of biotin-labelled HA binding to cell surface and analysis of physicochemical parameters of model membranes.

The cell membrane as the barrier in the defense against nanoxenobiotics: Zinc oxide nanoparticles interactions with native and model membrane of melanoma cells

“…This method allows for a simplified, hierarchical presentation and interpretation of the mechanical and structural properties of the outermost part of the cell, first in “simple” models (single‐component systems) and then in multicomponent systems (imitating the native membrane). The possibility of creating and using a single membrane layer reflecting the cell membrane with this technique and the analysis of its behavior through physicochemical parameters has been proven earlier (Gong et al, 2002; Hąc‐Wydro & Dynarowicz‐Łątka, 2008, Barbasz et al, 2016; Czyżowska et al, 2020; Dyba et al, 2021; Rudolphi‐Szydło et al, 2020). There is a strong correlation between the monolayer and the natural bilayer of the cell.…”

Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number

“…Nonadherent hematopoietic cells are frequently recognized as ex vivo sources of protein-based bioproducts applied in biotechnology, and biomedicine as well. Up to now, HL-60 cells as valuable human cells, have been applied as model in many studies on blood cells differentiation and physiology [ 14 – 17 ], or as a simple in vitro model in determination of cytotoxicity of anti-leukemic drugs [ 18 , 19 ]. But the efficiency of in vitro cultures of suspended biomass of HL-60 cells has not been extensively discussed so far, and the growth of HL-60 cells maintained in disposable bioreactor, as well as the influence of hydrodynamic conditions of the wave-type agitation on proliferation of HL-60 cells, has not been deeply studied or analysed up to date.…”

“…However, hematopoietic cells are an acknowledged ex vivo source of bioproducts with many potential applications in bone marrow transplantation, immunotherapy, gene therapy, as well as the source of blood-derived products [13]. A HL-60 cell line, i.e., human acute promyelocytic leukemia cells, has been frequently applied as model of hematopoietic cells, and it was used in studies focused on in vitro blood cell formation [14], their physiology [15] and differentiation [16,17], as well as to studying the cytotoxicity effects of potential anti-leukemic drugs [18], or even in lab-on-a-chip applications [19]. Thus, an enhancement of HL-60 cells' propagation efficiency is a current research issue highly expected by representatives of a range of application niches, which experimentally involve such type of cells.…”

Effects of 3FTx Protein Fraction from Naja ashei Venom on the Model and Native Membranes: Recognition and Implications for the Mechanisms of Toxicity

“…The isotherms of the surface pressure (π) of monolayers as a function of the area per lipid molecule were obtained by using the Langmuir through (Minitrough, KSV, Finland) equipped with a Pt-Wilhelmy plate. Single-lipids and mixtures solutions were applied onto PBS subphase and left for 15 min according to the measurement procedure described previously [ 67 , 68 , 69 ]. 3FTx were spread with the Hamilton microsyringe (± 50μL)) at the established concentrations.…”