The Digital Cytocochleogram

Santi, Peter A.; Blair, Amy; Bohne, Barbara A.; Lukkes, J.; Nietfeld, J.

doi:10.1016/j.heares.2004.01.017

Cited by 11 publications

(5 citation statements)

References 8 publications

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“…However, apoptotic cells were more prominently distributed in the base of the cochlea (6, 17). There was specifically damaged site by noise in the total length of the cochlea and the active apoptotic site mainly moved to the base of the cochlea.…”

Section: Discussionmentioning

confidence: 99%

“…The Digital Cytocochleogram in C57BL/J mice most similar with the relation of hearing loss patterns by noise and the total length of organ of Corti in BALB/c mice with the rest of the resources of the MCD is posted on the MCD website, currently located at http://mousecochlea.ccgb.umn.edu (17). In this database, 16 and 32 kHz correspond to 58.8% and 75.8% distance from the apex of the cochlea, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Apoptotic Pattern of Cochlear Outer Hair Cells and Frequency-specific Hearing Threshold Shift in Noise-exposed BALB/c Mice

Lim

Choi

Kang

et al. 2008

Clin Exp Otorhinolaryngol

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ObjectivesApoptosis of outer hair cell (OHC) can be identified through nuclear staining by specific nuclear changes. The change of filamentous actin (F-actin) is also involved in early cell death process. The study was designed to investigate OHC death along the whole length of the organ of Corti.MethodsBALB/c hybrid mice were used in this study. The noise group was exposed to white noise of 120 dB SPL for 3 hr per day for 3 consecutive days. The tone burst auditory brainstem response (ABR) test was conducted and cochleas from each group were obtained for the immunostaining of FITC phalloidin for F-actin and propidium iodide (PI) for nuclei.ResultsABR threshold of the noise group significantly increased after noise exposure (P<0.001). No threshold shift was found in the control group. Threshold shift of the noise group constantly increased from 4 to 16 kHz, but threshold shifts at 16 kHz and 32 kHz were similar. Patterns of OHC staining were subclassified as FITC+PI- cells, FITC+ PI+ cells, FITC-PI+ cells and missing cells. Proportion of normal live OHCs (FITC+PI-) rapidly decreased from the apex to the base. In the basal turn, FITC-PI+ cells and vacancy OHC (missing cells) were observed easily. Apoptotic and missing cells were most abundant at 60% of the whole length of the Corti organ.ConclusionWe could subclassify morphologic changes in OHC death after noise exposure. Quantitative changes in OHCs along the whole Corti organ showed a plateau pattern similar to that of a frequency-specific threshold shift.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Apoptotic Pattern of Cochlear Outer Hair Cells and Frequency-specific Hearing Threshold Shift in Noise-exposed BALB/c Mice

Lim

Choi

Kang

et al. 2008

Clin Exp Otorhinolaryngol

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“…A cytocochleogram was therefore constructed by calculating the proportion of hair cells that had survived of the total expected in each field according to the normal pattern. Since each field contained 0.32 mm of the length of the organ of Corti, we then plotted hair‐cell survival as a function of percentage distance from the apex in 16 consecutive fields representing 5.12 mm (or 84%) of the total length of approximately 6.04 mm 10 …”

Section: Methodsmentioning

confidence: 99%

“…Since each field contained 0.32 mm of the length of the organ of Corti, we then plotted hair-cell survival as a function of percentage distance from the apex in 16 consecutive fields representing 5.12 mm (or 84%) of the total length of approximately 6.04 mm. 10 Statistical analysis of data within and between groups was performed with ANOVA for repeated measures (two-way) followed by Bonferroni test using SigmaStat version 3.1 (Systat Software Inc., CA, USA). Changes within and between groups were considered statistically significant when the P value was less than 0.05.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Isoflurane Anesthesia on Noise‐Induced Hearing Loss in Mice

et al. 2005

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“…For the whole cochlea, Burda 37 found an average of 360. As described by Santi 28 and colleagues, [46][47][48] cell counting of the entire cochlea is extremely time-consuming. Identifying and counting all of the cochlear hair cells on serial sections of imbedded and processed slides under a light microscope can add weeks to months onto the study timeline depending on the number of trained technicians and available microscopes dedicated to the project.…”

Section: Cytocochleogram Parameters For Regulatory Reviewmentioning

confidence: 99%

Defining “Best Practices” For Critical Endpoints In Preclinical Screening of New Chemical Entities For Ototoxicity Liability

Gauvin¹,

Yoder²,

Tapp³

et al. 2016

Otolaryngol Open J

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CitationReview ABSTRACT Introduction: Ototoxicity has been defined as the tendency of certain therapeutic agents and other chemical substances to cause functional impairments and cellular degeneration of the tissues of the inner ear resulting in hearing loss. Objectives: This review is intended to provide details of a standardized preclinical assessment for ototoxicity under the current US FDA guidance documents that represents "industry best practices" for new drug application review of all new chemical entities being developed for human use. Methods: A literature review was conducted to assimilate study strategies that represent "Industry Best Practices" for the conduct of preclinical ototoxicity evaluation for submission to regulatory drug approval agencies. Conclusion: We have proposed a systems-approach and protocol criteria for the valid and reliable assessment of auditory function that can be easily included as a screening tool for ototoxicity within the Tiered Structure of preclinical assays required for approval of any chemical entity targeted for human use.

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The Digital Cytocochleogram

Cited by 11 publications

References 8 publications

Apoptotic Pattern of Cochlear Outer Hair Cells and Frequency-specific Hearing Threshold Shift in Noise-exposed BALB/c Mice

Apoptotic Pattern of Cochlear Outer Hair Cells and Frequency-specific Hearing Threshold Shift in Noise-exposed BALB/c Mice

Protective Effect of Isoflurane Anesthesia on Noise‐Induced Hearing Loss in Mice

Defining “Best Practices” For Critical Endpoints In Preclinical Screening of New Chemical Entities For Ototoxicity Liability

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