The Digestible Methionine and Cystine Requirements for Commercial Layers

Cao, T. Êh .; Weil, Jordan; Maharjan, Pramir; Lu, Jin‐Jian; Coon, C. N.

doi:10.3923/ijps.2020.232.243

Cited by 2 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could be associated with the hatching stress on the embryos, considering chick embryos may need more amino acids for energy via gluconeogenesis. Cao et al (1995) showed a similar specific activity for MAT in 35 day-old broilers. The current observation in hepatic MAT in hatched chicks agrees with the previous results in rats and mice where a peak and decaying enzyme activity was observed (Chase et al, 1968;Hancock, 1966).…”

Section: Discussionmentioning

confidence: 59%

“…Refsum et al (1989) reported also about 30% free homocysteine found in human plasma. This may indicate that the liver is more actively involved in methionine metabolism than other tissues, considering the liver is the only tissue that contains all enzymes and pathways (Cao et al, 1995;Finkelstein, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…The CBS assay conditions have been previously described by Mudd et al (1965) and Cao et al (1995). Enzyme extracts were incubated for 135 min at 37°C in the presence of Tris-HCl buffer, EDTA, pyridoxal 5′-phosphate, l-cystathionine, l-homocysteine, and [3-14 C]-l-serine.…”

Section: Enzyme Assaysmentioning

confidence: 99%

“…Additionally, impaired chick embryonic development has been associated with altered homocysteine levels (Rosenquist & Finnell, 2001). Cao et al (1995) evaluated the tissue distribution of MAT, CBS, and C-ase in broiler chicks. The researchers reported that enzyme activities and metabolites from these enzymes were affected by dietary methionine and cysteine.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ontogeny of hepatic methionine catabolic enzyme activities (Transmethylation and Transsulphuration) and associated physiological amino acids in E10‐21 chick embryos and D1‐49 broilers

Jin

Weil

Cerrate³

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

Developmental changes in hepatic methionine adenosyltransferase, cystathionine β-synthase, cystathionase, and glycine N-methyltransferase were determined in broiler chick embryos and hatched chicks by using radiometric and spectrometric methods. Hepatic free methionine, S-adenosylmethionine, S-adenosylhomocysteine, homocysteine, cystathionine, and cysteine levels were also investigated. Results showed an increase in hepatic MAT activity from E10 to E21 during embryogenesis, suggesting greater transmethylation rates throughout the rapid embryonic growth and development period. A strong positive correlation between embryo BW and How to cite this article: Lu J, Weil JT, Cerrate S, Coon CN. Ontogeny of hepatic methionine catabolic enzyme activities (Transmethylation and Transsulphuration) and associated physiological amino acids in E10-21 chick embryos and D1-49 broilers.

show abstract

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Enzyme Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ontogeny of hepatic methionine catabolic enzyme activities (Transmethylation and Transsulphuration) and associated physiological amino acids in E10‐21 chick embryos and D1‐49 broilers

Jin

Weil

Cerrate³

et al. 2020

Animal Physiology Nutrition

View full text Add to dashboard Cite

show abstract

“…Owing to its involvement in metabolic processes and oxidative stress, a nutritional imbalance of methionine can lead to a variety of metabolic problems. For instance, prior studies have suggested that altered methionine supplies impair normal methionine metabolism in laying hens and broilers and methionine metabolism plays an important role in chick embryo growth and development ( Cao et al., 1995 ; Lu et al., 2020a , 2020b ). In addition, Rosenquist and Finnell reported that increased homocysteine levels impaired chick embryonic development.…”

Section: Introductionmentioning

confidence: 99%

The effect of feeding adequate or deficient vitamin B6 or folic acid to breeders on methionine metabolism in 18-day-old chick embryos

et al. 2021

Self Cite

View full text Add to dashboard Cite

Three isotopic tracers ([2,3,3- 2 H 3 ]-L-serine, [ 2 H 11 ]-L-betaine, and [1- 13 C]-L-methionine) were administered by amnion injection into 18-day-old chick embryos to investigate the kinetics of methionine metabolism. The embryos utilized were from eggs collected from 34-week-old Cobb 500 broiler breeders that were fed either a control diet containing folic acid (1.25 mg/kg diet) and pyridoxine HCl (5 mg/kg diet) or diets devoid of supplemental pyridoxine or folic acid. Intermediate metabolites of methionine metabolism and polyamines were analyzed in 18-day-old chick embryos. There were no differences in hepatic [ 2 H 2 ] methionine or [ 2 H 3 ] cysteine enrichments or in physiological concentrations of sulfur amino acids for chick embryos from breeders fed the control diet and embryos from breeders fed diets containing no pyridoxine or folic acid. Supplementation of B 6 or folic acid did not affect the production of methionine and cysteine in chick embryos. However, breeders fed the control diet with both folic acid and pyridoxine supplementation produced embryos with a two-fold reduction of hepatic homocysteine and increased spermine compared with embryos from breeders fed diets containing no supplemental pyridoxine or folic acid ( P < 0.05). Hepatic S-adenosylmethionine for embryos from breeders fed no supplemental B 6 was half the concentration compared with embryos from breeders fed the control diet. Embryos from breeders fed the control diet were utilized to determine the proportion of homocysteine going through remethylation and transsulfuration and also to determine the pathway of remethylation. Sixty-five percent of the methyl groups used for homocysteine remethylation from control embryos was via the MFMT pathway. Alternatively, 61% of homocysteine from control embryos was remethylated via the MFMT and the BHMT reactions and 39% of homocysteine was catabolized to cysteine via the transsulfuration pathway. These data show that in embryos, intermediate metabolites of methionine and polyamines increase in concentration when pyridoxine levels are provided in deficient concentrations to the breeder hen. In addition, this research demonstrates that folic acid deficient embryos conserve methionine, rather than catabolize it to cysteine.

show abstract

The Digestible Methionine and Cystine Requirements for Commercial Layers

Cited by 2 publications

References 33 publications

Ontogeny of hepatic methionine catabolic enzyme activities (Transmethylation and Transsulphuration) and associated physiological amino acids in E10‐21 chick embryos and D1‐49 broilers

Ontogeny of hepatic methionine catabolic enzyme activities (Transmethylation and Transsulphuration) and associated physiological amino acids in E10‐21 chick embryos and D1‐49 broilers

The effect of feeding adequate or deficient vitamin B6 or folic acid to breeders on methionine metabolism in 18-day-old chick embryos

Contact Info

Product

Resources

About