The differentiation of normal and transformed human fibroblasts in vitro is influenced by electromagnetic fields

Rodemann, H. Peter; Bayreuther, Klaus; Pfleiderer, Gerhard

doi:10.1016/0014-4827(89)90263-2

Cited by 92 publications

(51 citation statements)

References 14 publications

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“…Nevertheless, this radiation points to interactions of biological importance. Rodemann et al [7] and scientists in the field of cell biology, reported accelerated aging and differentiation of skin cells by low energy electromagnetic fields [7]. But also the influence of mobile phones on the human health has to be considered as reported by Hyland [8] a pupil of Herbert Fröhlich.…”

Section: The Earth's Electromagnetic Spectrum and The Interaction Of mentioning

confidence: 99%

“…The old fibroblasts are not able to divide any more. A highly complex fibroblastic model system for cell differentiation, aging and cancer was developed by the German cell biologist Klaus Bayreuther at the end of the 1980s [7,15]. Hayflick [13] was the pioneer for fibroblastic aging models in the early 1960s.…”

Section: Cell Cultures As Important Tools For Cell Research On Agingmentioning

confidence: 99%

“…Cells can be grown as tissue cultures outside the organism, a technique developed by Alexis Carrel (1873-1944), the Nobel Prize winner for medicine and physiology in 1912 [1]. Although this famous French scientist claimed that cell cultures are immortal, Leonard Hayflick and his co-worker Paul Moorhead have reported in the 1960's that fibroblasts are aging and have a limited number of cell divisions before death as summarized before [6,7,13]. Last but not least, cell cultures have gained in importance recently for biological, biochemical and medical investigations of animals and humans.…”

Section: Cell Cultures As Important Tools For Cell Research On Agingmentioning

confidence: 99%

“…Since this time human diploid fibroblasts have become a widely accepted in vitro model system. Bayreuther and co-workers extended this experimental approach showing that fibroblasts in culture resemble, in their design, the hemopoietic stem-cell differentiation system [7,15]. In their reports morphological and biochemical evidence for the fibroblast stem-cell differentiation system in vitro was elaborated.…”

Section: Cell Cultures As Important Tools For Cell Research On Agingmentioning

confidence: 99%

“…Based on the stable field strength of its waves, they are also able to superpose. The light field of such a laser exhibits a high degree of order and therefore is able to generate order and to transfer information in living systems [2,7,20].In the middle of the nineties of the last century, Fritz-Albert Popp and the chemist Sophie Cohen [24] measured for the first time light emission on man. They showed biological daily rhythms by biophotonic measurements on the hands and the front of a human individual.…”

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Biophotons: Ultraweak Light Impulses Regulate Life Processes in Aging

Niggli¹

2014

J Gerontol Geriatric Res

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As shown by the history of light, photons participate in most atomic and molecular interactions. Recent biophysical research has measured low light impulses, so-called biophotonic emission, in cells and biological tissue. It is reported throughout the world that all cells (plant, animal or human) emit a weak, so-called biophotonic radiation. Based on the photoelectric effect, appropriate photomultiplier systems have been developed in order to detect this very weak light. Although the emission is extremely low in mammalian cells, it can be efficiently induced by light leading to delayed luminescence or light induced ultraweak photon re-emission. Re-emitted photons in cells are coupled with radical reactions and are probably also linked with the DNA as an important source. In recent years, cell culture models for biophotonic measurements using fibroblastic differentiation were generated and were used as an example to test the growth stimulation efficiency of various bone cell growth factors. It is well known that fibroblasts play an essential role in skin aging, skin carcinogenesis and wound healing. Therefore the biophotonic model of cells provides a new and powerful non-invasive tool for the development of new strategies in aging research. History of LightThe high importance of electromagnetic radiation for human life is well known. Following winter season, life on our earth retrieves vitality in early spring mostly by the sunrays reaching the ground. When white light enters a prism, it will divide into the well-known spectral colours: From red to orange, followed by yellow, changing to green as well as blue and finally showing the violet dye. The scientist Isaac Newton (1642-1727) noticed this already in his mid-twenties [1]. This observation indicated that white light was a composition of the rainbow colours. Johann Wolfgang von Goethe (1749-1832) confirmed this discovery. The light spectrum is not exclusively detected in the rainbow. In the action of forging one can notice that iron, once it is heated by the fire, switches colours from dark to bright red. It ultimately alternates at last into white. Although not combustible, solid matter behaves in this manner. From this observation, it is clear that long-wavelengths appear at lower temperatures. As the temperature elevates, shorter wave-lengths are noticed until all wave-lengths are present in the incandescent substance. Examinations on extremely hot stars have demonstrated that long wave-lengths steadily vanish and the colour changes into the blue spectrum range. The quantum theory of light is based on the scientific findings of Max Planck (1858Planck ( -1947 as well as Albert Einstein (1879Einstein ( -1955 and clarifies the nature of light [1]. They demonstrated the particle-and wave-like qualities of light. In 1803, the scientist Thomas Young (1773-1829) showed evidence of interference patterns in visible radiation [1]. His experimental testing demonstrated that visible radiation is based on waves. This has already been shown by Christiaan Huygen...

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Section: The Earth's Electromagnetic Spectrum and The Interaction Of mentioning

confidence: 99%

Section: Cell Cultures As Important Tools For Cell Research On Agingmentioning

confidence: 99%

Section: Cell Cultures As Important Tools For Cell Research On Agingmentioning

confidence: 99%

Section: Cell Cultures As Important Tools For Cell Research On Agingmentioning

confidence: 99%

mentioning

confidence: 99%

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Biophotons: Ultraweak Light Impulses Regulate Life Processes in Aging

Niggli¹

2014

J Gerontol Geriatric Res

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Effects of extremely low frequency electromagnetic field (EMF) on collagen type I mRNA expression and extracellular matrix synthesis of human osteoblastic cells

Heermeier¹,

Spanner²,

Trager

et al. 1998

Bioelectromagnetics

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Human osteoblastic cells were grown in a three‐dimensional (3‐D) cell culture model and used to test the effects of a 20 Hz sinusoidal electromagnetic field (EMF; 6 mT and 113 mV/cm max) on collagen type I mRNA expression and extracellular matrix formation in comparison with the effects of growth factors. The cells were isolated from trabecular bone of a healthy individual (HO‐197) and from a patient presenting with myositis ossificans (MO‐192) and grown in a collagenous sponge‐like substrate. Maximal enhancement of collagen type I expression after EMF treatment was 3.7‐fold in HO‐197 cells and 5.4‐fold in MO‐192 cells. Similar enhancement was found after transforming growth factor‐β (TGF‐β) and insulin‐like growth factor‐I (IGF‐I) treatment. Combined treatment of the cells with EMF and the two growth factors TGF‐β and IGF‐I did not act synergistically. MO‐192 cells produced an osteoblast‐characteristic extracellular matrix containing collagen type I, alkaline phosphatase, and osteocalcin, together with collagen type III, TP‐1, and TP‐3, two epitopes of an osteoblastic differentiation marker. The data suggest that the effects of EMFs on osteoblastic differentiation are comparable to those of TGF‐β and IGF‐I. We conclude that EMF effects in the treatment of skeletal disorders and in orthopedic adjuvant therapy are mediated via enhancement of collagen type I mRNA expression, which may lead to extensive extracellular matrix synthesis. Bioelectromagnetics 19:222–231, 1998. © 1998 Wiley‐Liss, Inc.

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Effect of 50 Hz sinusoidal electromagnetic field on the kinetics of14CO2 exhalation after [14C]-N-Nitrosodiethylamine administration in mice

Singh

Khanduja

Mittal

1999

Bioelectromagnetics

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N‐Nitrosodiethylamine (NDEA) has been identified as a typical environmental carcinogen. Its metabolism was studied in mice under the influence of an electromagnetic field (EMF). After intraperitoneal administration of [14C]‐NDEA, 0.2 μCi/100 g body weight resulted in 22.8% of the total radioactivity exhaled as 14CO2 within 1 h. Mice were exposed to a 50 Hz, 2 mT (rms) electromagnetic field, 8 h/day for 8 weeks. There was a significant increase in the metabolic turnover of [14C]‐NDEA into 14CO2 at the end of both 6 and 8 weeks of field exposure, i.e., 26.9% and 37.4% respectively. The enhanced capacity of mice to metabolize NDEA after the exposure to EMF may result in animals with a smaller amount of the bioactive carcinogen burden, thereby indicating a protective role of 2 mT EMF in a whole animal study. Bioelectromagnetics 20:1–4, 1999. © 1999 Wiley‐Liss, Inc.

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The differentiation of normal and transformed human fibroblasts in vitro is influenced by electromagnetic fields

Cited by 92 publications

References 14 publications

Biophotons: Ultraweak Light Impulses Regulate Life Processes in Aging

Biophotons: Ultraweak Light Impulses Regulate Life Processes in Aging

Effects of extremely low frequency electromagnetic field (EMF) on collagen type I mRNA expression and extracellular matrix synthesis of human osteoblastic cells

Effect of 50 Hz sinusoidal electromagnetic field on the kinetics of14CO2 exhalation after [14C]-N-Nitrosodiethylamine administration in mice

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