The differential viscometer. II. On‐line viscosity detector for size‐exclusion chromatography

Abstract

SynopsisThe new technique of differential viscometry measures directly the specific viscosity of a solution by subtracting the contribution of solvent in a balanced capillary bridge. The present work adapts the differential viscometry principle to the design of a viscosity detector for use in size-exclusion chromatography. It is shown that the resulting viscosity detector possesses excellent sensitivity and baseline stability with a minimum detectable specific viscosity of 2.7x The viscosity detector can be operated together with a refractive index detector to determine the intrinsic viscosity of polymer solute fractions as they elute from the SEC column.The bandspreading of the viscosity detector is compared to the refractive index detector by measuring the peak width of a compound having a single discrete molecular weight. The peak width at half-height was 0.29 mL for the viscosity detector and 0.25 mL for the refractive index detector.

Characterization and analysis of thermal denaturation of antibodies by size exclusion high-performance liquid chromatography with quadruple detection

“…Light scattered at a 90°a ngle and intrinsic viscosity measured by the four-capillary differential viscometer are used to determine size and structure of proteins and polymers. Haney [14][15][16] describes the theory, principle, and operation of differential viscometers in several reviews. The theory behind the use of LS and RI signals for calculation of molecular weight distribution is described in detail in several of the earlier reports and reviews [8][9][10][11]17].…”

Studying Abnormal Viscosity Behavior in Dilute Oligomer Solutions by Sec and Rheology

“…As shown in the figure, this configuration is a fluid flow analog of the Wheatstone bridge electrical circuit. [11,12] The differential pressure transducer measures the change in pressure across the bridge (DP), while the inlet pressure transducer measures the pressure change through the bridge (IP). The polymer 2008 STRIEGEL AND ALWARD ©2002 Marcel Dekker, Inc. All rights reserved.…”

Static and dynamic characteristics of a hydraulic Wheatstone bridge mass flowmeter

“…The frequency characteristics of the flowmeter were experimentally studied in [7]. With a similar configuration to a Wheatstone bridge mass flowmeter, the differential viscometer, which offers several advantages for measuring the viscometry of polymer solutions, has also been developed [8,9].…”