The differential regulation of osteoblast and osteoclast activity by surface topography of hydroxyapatite coatings

Costa, Daniel O.; Prowse, Paul; Chrones, Tom; Sims, Stephen M.; Hamilton, David A.; Rizkalla, Amin S.; Dixon, S. Jeffrey

doi:10.1016/j.biomaterials.2013.06.014

Cited by 198 publications

(149 citation statements)

References 54 publications

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“…PRF incorporation appeared to increase the surface roughness of the scaffolds, which may promote cellular adhesion. 44 The nonprinted and printed BCP/PVA/PRF scaffolds had lower contact angles than the nonprinted and printed BCP/PVA scaffolds, respectively (P,0.05; Figure 2K), demonstrating that the incorporation of PRF improved the hydrophilicity of the scaffolds. The 3D printed BCP/PVA and BCP/PVA/PRF scaffolds (49.6 and 54.7%) had significantly higher total porosities than the nonprinted BCP/PVA and BCP/PVA/PRF scaffolds (1.5 and 6.1%, P,0.05), respectively ( Figure 2L), confirming that the 3D printing process increased the porosity of the scaffolds, which may enhance cellular penetration and distribution to facilitate vascularization.…”

Section: Porosity (%)mentioning

confidence: 94%

Nano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin

Song

Lin

et al. 2018

IJN

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Background and aim As a newly emerging three-dimensional (3D) printing technology, low-temperature robocasting can be used to fabricate geometrically complex ceramic scaffolds at low temperatures. Here, we aimed to fabricate 3D printed ceramic scaffolds composed of nano-biphasic calcium phosphate (BCP), polyvinyl alcohol (PVA), and platelet-rich fibrin (PRF) at a low temperature without the addition of toxic chemicals. Methods Corresponding nonprinted scaffolds were prepared using a freeze-drying method. Compared with the nonprinted scaffolds, the printed scaffolds had specific shapes and well-connected internal structures. Results The incorporation of PRF enabled both the sustained release of bioactive factors from the scaffolds and improved biocompatibility and biological activity toward bone marrow-derived mesenchymal stem cells (BMSCs) in vitro. Additionally, the printed BCP/PVA/PRF scaffolds promoted significantly better BMSC adhesion, proliferation, and osteogenic differentiation in vitro than the printed BCP/PVA scaffolds. In vivo, the printed BCP/PVA/PRF scaffolds induced a greater extent of appropriate bone formation than the printed BCP/PVA scaffolds and nonprinted scaffolds in a critical-size segmental bone defect model in rabbits. Conclusion These experiments indicate that low-temperature robocasting could potentially be used to fabricate 3D printed BCP/PVA/PRF scaffolds with desired shapes and internal structures and incorporated bioactive factors to enhance the repair of segmental bone defects.

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Section: Porosity (%)mentioning

confidence: 94%

Nano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin

Song

Lin

et al. 2018

IJN

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“…One possible 10µm 7 explanation is that the high roughness of the substrate prevented the OC from sealing the substrate, thus hampering the degradation process, as described in previous works. 6,25 One aspect worth noticing is the fact that the microstructure of the pristine material was revealed intact beneath the cell, thus confirming that there was no damage associated to FIB-sectioning.…”

Section: Resultsmentioning

confidence: 78%

“…5 Several pre-clinical studies of bone substitutes have focused on these early interactive stages and, particularly, on the biomaterial-osteoclasts interactions in terms of OC adhesion, gene expression and evaluation of resorption pits. [6][7][8] Usually, these investigations require, on the one hand, the study of OC differentiation and activity, such as gene expression or marker identification, and on the other hand, the evaluation of morphological changes in the substrates, which implies staining or removal of cells in order to visualize the underlying structure. RANKL-containing cell culture medium (DMEM) from human osteoblast supernatants.…”

Section: Introductionmentioning

confidence: 99%

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

Díez-Escudero

Español

Montúfar

et al. 2017

Tissue Engineering Part C: Methods

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“…Another relevant aspect is that not only the chemistry, but also the surface topography was different in the two robocasted scaffolds. There is extensive literature that indicates that different cell responses can be expected towards hydroxyapatite substrates with varying micro and nanoscale surface topographies 37,42 as the ones developed in the present study, and work in this direction is under way in our laboratories.…”

mentioning

confidence: 94%

Robocasting of biomimetic hydroxyapatite scaffolds using self-setting inks

et al. 2014

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Low temperature self-setting ceramic inks have been scarcely investigated for solid freeform fabrication processes. This work deals with the robocasting of alpha-tricalcium phosphate/gelatine reactive slurries as a bioinspired self-setting ink for the production of biomimetic hydroxyapatite/gelatine scaffolds. A controlled and totally interconnected pore network of $300 mm was obtained after ink printing and setting, with the struts consisting of a micro/nanoporous matrix of needle-shaped calcium deficient hydroxyapatite crystals, with a high specific surface area. Gelatine was effectively retained by chemical crosslinking. The setting reaction of the ink resulted in a significant increase of both the elastic modulus and the compressive strength of the scaffolds, which were within the range of the human trabecular bone. In addition to delaying the onset of the setting reaction, thus providing enough time for printing, gelatine provided the viscoelastic properties to the strands to support their own weight, and additionally enhanced mesenchymal stem cell adhesion and proliferation on the surface of the scaffold. Altogether this new processing approach opens good perspectives for the design of hydroxyapatite scaffolds for bone tissue engineering with enhanced reactivity and resorption rate.

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The differential regulation of osteoblast and osteoclast activity by surface topography of hydroxyapatite coatings

Cited by 198 publications

References 54 publications

Nano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin

Nano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin

Focus Ion Beam/Scanning Electron Microscopy Characterization of Osteoclastic Resorption of Calcium Phosphate Substrates

Robocasting of biomimetic hydroxyapatite scaffolds using self-setting inks

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