The differential plasma and ruminal metabolic pathways and ruminal bacterial taxa associated with divergent residual body weight gain phenotype in crossbred beef steers

Idowu, Modoluwamu; Taiwo, Godstime; Sidney, Taylor; Olanrewaju, Morenikeji; Pech-Cervantes, Andres A; Estrada-Reyes, Zaira M; Wilson, Matthew E.; Ogunade, Ibukun M

doi:10.1093/tas/txad054

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…Several studies have sought to understand how selection for divergent RFI or RADG affects important traits such as metabolism, fertility, rumen microbiome, and carcass characteristics (Cantalapiedra-Hijar et al, 2018;Taiwo et al, 2022;Idowu et al, 2023). In our recent study, Taiwo et al (2023) investigated expression of immunity-associated genes in the liver and whole blood of crossbred beef cattle with divergent RFI.…”

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confidence: 99%

Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain

Ologunagba,

Idowu,

Taiwo

et al. 2024

Front. Anim. Sci.

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Immune function plays a pivotal role in dictating the overall health and productivity of cattle. In a proficient immune system, the liver assumes an integral function in detoxification and metabolic processes and contributes substantially to overall production and immunity. In this study, we evaluated the hepatic mRNA expression of genes involved in innate and adaptive immunity in crossbred beef steers with positive or negative residual body weight gain (RADG). Positive-RADG beef steers (n = 8; RADG = 0.73 kg/d) and negative-RADG beef steers (n = 8; RADG = -0.69 kg/d) were identified from a group of 108 growing crossbred beef steers (average BW = 556 ± 38 kg) after a 56-d performance testing period. At the end of the 56-d period, liver tissue samples were collected from the beef steers for RNA extraction and cDNA synthesis. The mRNA expression of 84 genes involved in innate and adaptive immunity were analyzed using pathway-focused PCR-based arrays. The mRNA expression of genes with false discovery rate-adjusted P-values (FDR) ≤ 0.05 and absolute fold change (FC) ≥ 1.2 were determined to be differentially expressed. Out of the 84 genes analyzed, four genes (IL-2, MYD88, CD-80, NFkB-1) were differentially expressed and were all upregulated in positive compared with negative-RADG beef steers. IL-2 is a cytokine that plays a critical role in the immune response by activating and proliferating T-cells, which are important for fighting infections. MYD88 is an adaptor protein that is essential for signaling by toll-like receptors, which are involved in pathogen recognition. CD80 is a protein that is expressed on the surface of antigen-presenting cells and plays a critical role in the initiation of an immune response. The activation of NF-κB leads to the production of cytokines and chemokines that help to recruit immune cells to the site of infection. The upregulation of the aforementioned genes in positive-RADG beef steers suggests that they had a better ability than negative-RADG beef steers to quickly recognize pathogens and initiate appropriate responses to effectively fight off infections without causing inflammatory reactions, potentially contributing to their better feed efficiency.

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Section: Introductionmentioning

confidence: 99%

Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain

Ologunagba,

Idowu,

Taiwo

et al. 2024

Front. Anim. Sci.

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Rumen fermentation parameters and papillae development in Simmental growing bulls with divergent residual feed intake

Romanzin,

Braidot,

Beraldo

et al. 2024

animal

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Effects of dietary supplementation of a blend of Saccharomyces cerevisiae, multiple live probiotic bacteria, and their fermentation products on performance, health, and rumen bacterial community of newly weaned beef steers during a 56-d receiving period

Treon,

Sidney,

Taiwo

et al. 2023

Translational Animal Science

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We examined the effects of a blend of Saccharomyces cerevisiae, multiple live probiotic bacteria and their fermentation products on performance, health, and the ruminal bacterial community of newly weaned beef steers during a 56-day receiving period. Forty newly weaned Angus crossbred steers (221 ± 25.6 kg BW; 180 ± 17 d of age) were stratified by body weight into four pens (10 steers per pen) such that each pen had a similar average body weight at the beginning of the experiment. The pens were randomly assigned to receive a corn silage basal diet (CON; n = 20) or the basal diet supplemented with 9 g/steer/d of PRO feed additive (PRO; n = 20). The PRO additive is a blend of Saccharomyces cerevisiae and the fermentation products of Enterococcus faecium, Bacillus licheniformis, Bacillus subtilis, Lactobacillus animalis, Propionibacterium freudenreichii. The DMI and water consumed were monitored using the GrowSafe intake nodes and custom flow meters, respectively. Body weights (BW) were recorded weekly to calculate average daily gain (ADG). Before morning feeding, 10 mL of blood was taken from each steer on days 0-7, and thereafter weekly for analyses of immune cells, plasma glucose and NEFAs. On day 56, rumen fluid samples (200 mL each) were collected from all the steers for microbiome analysis. Over the 56-day receiving period, the supplemental PRO had no effects on DMI, water intake, or ADG. However, compared to CON, beef steers fed supplemental PRO tended to have greater ADG (P = 0.08) and BW (P = 0.07) during the first 14 days of the study. There was a treatment × day interaction (P ≤ 0.05) for WBC, neutrophils and monocytes over the 56 days such that beef steers fed supplemental PRO had lower blood concentrations on certain days during the first 7 days after weaning, indicating reduced inflammation or stress response. The results of the rumen microbiome analysis revealed that the relative abundance of complex fiber degrading or obligate proton-reducing bacterial genera such as Bacteroides, Ruminococcus gauvreauii group, Desulfovibrio, Syntrophococcus, and Acetitomaculum were greater (P ≤ 0.05) in beef steers fed supplemental PRO compared to CON. This study demonstrated that dietary supplementation of PRO improved the growth performance, reduced stress or inflammatory response during the initial days after weaning and altered the ruminal bacterial community towards increased relative abundance of bacterial genera associated with improved rumen function.

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The differential plasma and ruminal metabolic pathways and ruminal bacterial taxa associated with divergent residual body weight gain phenotype in crossbred beef steers

Cited by 3 publications

References 48 publications

Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain

Hepatic mRNA expression of innate and adaptive immune genes in beef steers with divergent residual body weight gain

Rumen fermentation parameters and papillae development in Simmental growing bulls with divergent residual feed intake

Effects of dietary supplementation of a blend of Saccharomyces cerevisiae, multiple live probiotic bacteria, and their fermentation products on performance, health, and rumen bacterial community of newly weaned beef steers during a 56-d receiving period

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