The Differential Expression of Adhesion Molecule and Extracellular Matrix Genes in Mesenchymal Stromal Cells after Interaction with Cord Blood Hematopoietic Progenitors

Abstract

The dynamics of the expression of genes encoding adhesion molecules, molecules of the connective tissue matrix, and its remodeling enzymes was studied in multipotent mesenchymal stromal cells (MSCs) from human adipose tissue after interaction with cord blood hematopoietic progenitors (HSPCs). An upregulation of ICAM1 and VCAM1, directly proportional to the coculture time (24-72 h), was found. After 72 h of culturing, a downregulation of the genes encoding the majority of matrix molecules (SPP1; COL6A2,7A1; MMP1,3; TIMP1,3; and HAS1) and cell-matrix adhesion molecules (ITGs) was revealed. The detected changes may ensure the realization of the stromal MSC function due to improvement of adhesion and transmigration of HSPCs into the subcellular space.

Co-Transplantation of Hypoxia Pretreated Human Adipose Derived Mesenchymal Stem Cells and Cord Blood Mononuclear Cells to Treat Rats With Acute Myocardial Infarction

“…HSPCs) for their viability and proliferation, through direct/ paracrine cell-to-cell interactions [34,35]. In addition, ASCs can also upregulate the adhesion molecule and extracellular matrix genes in HSPCs, which was good for the latter to anchor and survive in the local area [36].All aforementioned evidence demonstrated that the paracrine factors that secreted by ASCs might be used by CBMNCs for survival and function when they are co-transplanted simultaneously. So, we need to nd new strategies to improve the treatment e cacy.…”

Stem cells in the treatment of diabetes mellitus — Focus on mesenchymal stem cells

“…Hence, heterogeneous effects of MSC on GSIS may partly result from different expression levels of ANXA1. Likely, the very close proximity of MSC and islets in direct co-cultures enhanced local effector levels and involves the extracellular matrix (ECM) since MSC cocultured with HSC maintain the vascular niche by upregulated expression of intercellular adhesion molecule 1 (ICAM1) and vascular cell adhesion molecule 1 (VCAM1) [149]. Such supportive processes are an important topic in tissue engineering and it has been recognized that islets, which lost ECM during enzymatic isolation, show improved survival and function after treatment with ECM molecules [150].…”

Autologous Cord Blood in Children with Cerebral Palsy: A Review

“…Studies investigating the mutual interactions between stem cell subpopulations are also in their infancy. It has been shown that CB hematopoietic progenitors influence the expression of genes encoding for adhesion molecules and molecules of the connective tissue matrix [143]. Its remodeling enzymes was also studied in multipotent mesenchymal stromal cells (MSCs) from human adipose tissue [144].…”