The differential effects of ammonium and nitrate on methanotrophs in rice field soil

Hu, Ang; Lu, Yahai

doi:10.1016/j.soilbio.2015.02.033

Cited by 58 publications

(24 citation statements)

References 40 publications

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“…We measured soil physiochemical variables, namely, soil moisture, pH, total nitrogen (TN), total phosphorus (TP), total organic carbon (TOC), water-soluble soil organic carbon (WSOC), water-soluble soil organic nitrogen (WSON), NO 3 − -N, and NH 4 + -N, according to previous literatures [24,25].…”

Section: Climate and Soil Physiochemical Variablesmentioning

confidence: 99%

Mountain biodiversity and ecosystem functions: interplay between geology and contemporary environments

Wang

Sun

et al. 2020

The ISME Journal

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Although biodiversity and ecosystem functions are strongly shaped by contemporary environments, such as climate and local biotic and abiotic attributes, relatively little is known about how they depend on long-term geological processes. Here, along a 3000-m elevational gradient with tectonic faults on the Tibetan Plateau (that is, Galongla Mountain in Medog County, China), we study the joint effects of geological and contemporary environments on biological communities, such as the diversity and community composition of plants and soil bacteria, and ecosystem functions. We find that these biological communities and ecosystem functions generally show consistent elevational breakpoints at 2000-2800 m, which coincide with Indus-Yalu suture zone fault and are similar to the elevational breakpoints of soil bacteria on another mountain range 1000 km away. Mean annual temperature, soil pH and moisture are the primary contemporary determinants of biodiversity and ecosystem functions, which support previous findings. However, compared with the models excluding geological processes, inclusion of geological effects, such as parent rock and weathering, increases 67.9 and 35.9% of the explained variations in plant and bacterial communities, respectively. Such inclusion increases 27.6% of the explained variations in ecosystem functions. The geological processes thus provide additional links to ecosystem properties, which are prominent but show divergent effects on biodiversity and ecosystem functions: parent rock and weathering exert considerable direct effects on biodiversity, whereas indirectly influence ecosystem functions via interactions with biodiversity and contemporary environments. Thus, the integration of geological processes with environmental gradients could enhance our understanding of biodiversity and, ultimately, ecosystem functioning across different climatic zones.

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Section: Climate and Soil Physiochemical Variablesmentioning

confidence: 99%

Mountain biodiversity and ecosystem functions: interplay between geology and contemporary environments

Wang

Sun

et al. 2020

The ISME Journal

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“…Fertilization is an important agricultural practice for improving nutrition of plants, reaching high yield, and also for changes of soil environment-such as the chemistry of soil carbon and nitrogen [16], which can change the activity and diversity of soil microorganisms [17,18]. Former studies have shown that fertilization influences and changes to the microbial community in nitrogenous bacteria [19], methanotrophs [20], and cellulotic bacteria [21]. Microorganisms may react to changing conditions of soil environment by adjustment of composition in microbial community and total quantity of microbial biomass [22].…”

Section: Introductionmentioning

confidence: 99%

Soil Microbial Communities and Enzyme Activities after Long-Term Application of Inorganic and Organic Fertilizers at Different Depths of the Soil Profile

et al. 2019

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Fertilization is a key factor for sustaining productivity in agroecosystems. A long-term experiment in cambisol following periodical application of several types of fertilization has been running at the experimental site since 1954. In this study, we determined the impact of applied inorganic and/or organic fertilizers on the activity of soil enzymes and on the structure of microorganisms at depths of 0–30 cm and 30–60 cm. Single-factor comparison showed that use of inorganic and/or organic fertilizer had an insignificant effect on the activities of soil enzymes (at depths 0–30 cm and 30–60 cm) and also on the structure of microbial communities at both depths studied. Only soil respirations exhibited stimulation by combined fertilization. The results, irrespective of sampling depth (0–60 cm), showed that application of combined organic and inorganic fertilization stimulated the activity of glucosidases and use of inorganic fertilizer inhibited the activity of arylsulphatases. Respirations were stimulated by application of organic fertilizer and combined fertilization. Nevertheless, principal component analyses, which calculate with multidimensional data, revealed differences in samples treated by sole mineral fertilizer compared to other variants, especially in the lower layer. In general, our results indicate that use of combined fertilization may improve biological characteristics in deeper parts of soil profile and possibly increase biological activity in agroecosystems.

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“…It was generally regarded that ammonium radicals were the competitive inhibitor of particular methane monooxygenase (pMMO) (He et al, 2017; Hu and Lu, 2015; Dam et al, 2014; Nyerges et al, 2010; Nyerges and Stein, 2009; Nyerges, 2008; Dunfield and Knowles, 1995; Schnell and King, 1994; Carlsen et al, 1991; Murrellt and Dalton, 1983), which will resulted in the lower growth of strains from methane. However, this study also proved that ammonium would hinder the growth of ZR1 from methanol, although its supposed competent object pMMO is not the key enzyme for the metabolism of methanol.…”

Section: Resultsmentioning

confidence: 99%

Accumulation of ammonium owing to the metabolic imbalance of carbon and nitrogen might inhibit the central metabolism inMethylomonassp. ZR1

Zhao

et al. 2020

Preprint

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Abstract： 12The metabolic intermediates of nitrogen source have been proved to have 13 multiple functions on the metabolism of mehthanotrophs. In this study, 14 accumulation and assimilation mechanism of the nitrate metabolic 15 intermediate ammonium in the fast growing Methylomonas sp. ZR1 was 16 analyzed. Although, nitrate salt was the best nitrogen source supporting 17 the growth of ZR1, its metabolic intermediate ammonium would 18 accumulate and inhibit ZR1. Kinetic studies indicated that accumulation 19 of NH4 + was deduced from the imbalance of nitrogen and carbon 20 metabolism. Compensation of carbon skeleton α-keto-glutaramate could 21 effectively relieve the inhibition of NH4 + to ZR1, which further approved 22 42relieve the ammonium stress according to the edible carbon source. 43Revealing the endogenous ammonium accumulation mechanism and its 44 3 metabolic adjustment effect on the central metabolism of methanotrophs, 45 was meaningful to reveal the complex coordination metabolic 46 mechanism of nitrogen and carbon in methanotrophs. 47 Introduction： 49 Methanotrophs are excellent C1 compounds assimilating microorganisms, 50 and its industrial application has a long history since 1960s. At the 51 beginning methanotrophs were used to produce single cell protein from 52 methane or methanol. Nowadays, with the development of biotechnology, 53 methanotrophs were genetic modified to produce lactic acid (Henard et 54 al., 2016; Garg et al., 2018), astaxanthin (Ye and Kelly, 2012) and 55 α-hemelune (Sonntag et al., 2015) from methane. However, the slow 56 growth nature of methanotrophs has constrained its application in 57 industrial biotechnology. 58 Efforts to optimize fermentation technologies to improve the growth rates 59 of methanotrophs on methane have been reported over the past years (Yu 60 et al., 2003; Park et al., 1991b; Shah et al., 1996; Xing et al., 2006; Han et 61 al., 2009) (Myung et al., 2016). Other factors such as the addition of 62 citrate acid (Xing et al., 2006) and adjusting the ion concentration (Leak 63 and Dalton, 1986) were also reported to have effect in improving the 64 growth rate of methanotrophs from methane. These studies indicated that 65 the growth rate of methanotrophs might be result from multi-factor 66 4 combined effects. Many studies have also proved that nitrogen sources 67 have strong effect on the growth of methanotrophs. Park et al. studied 68 several factors including the nitrogen source affected the growth of OB3b, 69 and found that nitrate depletion was responsible for the diauxic growth 70 pattern in the batch cultivation of OB3b in the bioreactor. However, its 71 growth declined much with 40 mM nitrate (Park et al., 1991). Hoefman et 72 al. found niche partitioning among methanotrophic species, with methane 73 oxidation activity responses to changes in nitrogen content being 74 dependent on the in situ methanotrophic community structure (Hoefman 75 et al., 2014). Strains have developed a complex mechanism to balance the 76 carbon and nitrogen metabolism (Commic...

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The differential effects of ammonium and nitrate on methanotrophs in rice field soil

Cited by 58 publications

References 40 publications

Mountain biodiversity and ecosystem functions: interplay between geology and contemporary environments

Mountain biodiversity and ecosystem functions: interplay between geology and contemporary environments

Soil Microbial Communities and Enzyme Activities after Long-Term Application of Inorganic and Organic Fertilizers at Different Depths of the Soil Profile

Accumulation of ammonium owing to the metabolic imbalance of carbon and nitrogen might inhibit the central metabolism inMethylomonassp. ZR1

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