The Differential Diagnosis of Tumour Cells in Circulating Blood

Alexander, R. F.; Spriggs, A. I.

doi:10.1136/jcp.13.5.414

Cited by 86 publications

(34 citation statements)

References 13 publications

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“…This has been previously demonstrated by other investigators (1,9,16,25). Using the above described method, we studied the composition of the leucocyte concentrate in a group of 50 normal subjects (25 men aged 24-62 and 25 women aged 18-42) (figs.…”

Section: Resultssupporting

confidence: 73%

“…Mitotic figures are relatively often seen. The atypical mononuclear cells closely resemble plasma cells, and some in vestigators ( 1,48,50) consequently refer to them as atypical plasma cells. K l im a et al (21,23,25), on the other hand, assume that these cells originate from lymphocytes, and refer to them as lymphatic ' ' Reaktionsfor men' '.…”

Section: Resultsmentioning

confidence: 99%

“…Using the above described method, we studied the composition of the leucocyte concentrate in a group of 50 normal subjects (25 men aged 24-62 and 25 women aged 18-42) (figs. [1][2][3][4][5][6][7][8]. 67 1.…”

Section: Resultsmentioning

confidence: 99%

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The Significance of the Leucocyte Concentrate in the Demonstration of Tumour Cells in the Blood

Stofberg¹

1963

Acta Haematol

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

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The Significance of the Leucocyte Concentrate in the Demonstration of Tumour Cells in the Blood

Stofberg¹

1963

Acta Haematol

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“…While it seems like this burgeoning field has only recently blossomed, its roots date back as far as 1869, with the first reported description of CTCs by Thomas Ashworth (Ashworth, 1869). After a sparse trail of publications, early reports on methods for detecting CTCs via filtration (Salgado et al, 1959) and sedimentation (Alexander and Spriggs, 1960) emerged nearly 100 years later. Perhaps the most commonly used technique for detection today, immunomagnetic separation, was not reported until 1998 (Racila et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Circulating tumor cell technologies

2016

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Cancer CTC capture CTC clustersImmunoaffinity enrichment A B S T R A C TCirculating tumor cells, a component of the "liquid biopsy", hold great potential to transform the current landscape of cancer therapy. A key challenge to unlocking the clinical utility of CTCs lies in the ability to detect and isolate these rare cells using methods amenable to downstream characterization and other applications. In this review, we will provide an overview of current technologies used to detect and capture CTCs with brief insights into the workings of individual technologies. We focus on the strategies employed by different platforms and discuss the advantages of each. As our understanding of CTC biology matures, CTC technologies will need to evolve, and we discuss some of the present challenges facing the field in light of recent data encompassing epithelial-to-mesenchymal transition, tumor-initiating cells, and CTC clusters.

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“…reporting from the Brompton Hospital, describes the use of Ehrlich's acid haematoxylin and eosin for the examination of tumour cells in blood, whereas, for the same purpose, Alexander and Spriggs (1960) find Romanowsky stains useful. The present study was undertaken to evaluate fluorescence microscopy for cytological diagnosis using acridine orange as described by von Bertalanffy, Masin and Masin (1958).…”

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confidence: 99%

Fluorescence Microscopy in Exfoliative Cytology

Grubb¹,

Crabbe²

1961

Br J Cancer

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EXFOLIATIVE cytology is a diagnostic procedure, which, having been generally accepted, is growing rapidly in importance as a means of early diagnosis of cancer. The Papanicolaou technique, developed originally to investigate gynaecological cancer, has been extensively applied to material from various sources and hag collected a voluminous literature testifying to its usefulness, (Papanicolaou, 1954;Ayre, 1951; Schade, 1959). The diagnosis of the malignant cell however is, in the final analysis, subjective and it is experience and familiarity with a technique that is important, not the stain used. Philps (1954), using temporary wet films stained with methylene blue (Schuster, 1947) and permanent films stained with haemalum and eosin (Dudgeon and Wrigley, 1935), diagnosed 76-9 per cent carcinomas of lung by means of exfoliative cytology. Spriggs (1957) recommends May-Grundwald-Giemsa for the examination of serious fluids. Luckeock (1961), reporting from the Brompton Hospital, describes the use of Ehrlich's acid haematoxylin and eosin for the examination of tumour cells in blood, whereas, for the same purpose, Alexander and Spriggs (1960) find Romanowsky stains useful. The present study was undertaken to evaluate fluorescence microscopy for cytological diagnosis using acridine orange as described by von Bertalanffy, Masin and Masin (1958).Fluorescence microscopy has been applied to cytology using berberine sulphate, acid fuchsin and the acridine group of stains (Friedman, 1950; Mellors, Glassman and Papanicolaou, 1952). Its use is based on the affinity of the basic fluorochrome dyes for the nucleic acids. Studies of the neoplastic cell have shown an increase in the total nucleic acids and ribonucleic acid (RNA). This increase is characteristic of cells showing a high protein synthesis and is seen in embryonic, regenerating, secretory and malignant cells. The RNA is found in the cytoplasm and the nucleoli of the affected cells. Increase in deoxyribonucleic acid (DNA), found in the chromatin of the nucleus, is seen in cells undergoing mitosis and polyploidy characteristic of advanced cancer. The fluorescent techniques described by Friedman and others have proved tedious and inadequate.von Bertalanffy et al. (1958) have reviewed the literature on the role of nucleic acids in malignant cells, and the theoretical considerations for the use of basic fluorochrome stains and they have suggested the use of acridine orange in routine exfoliative cytology.Acridine orange (AO) is a histochemical fluorochrome with a selective affinity for nucleic acids. At a concentration of 0.01 per cent and a pH of 6, recommended by von Bertalanffy, the DNA fluoresces yellow to whitish green, and the RNA red.

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The Differential Diagnosis of Tumour Cells in Circulating Blood

Cited by 86 publications

References 13 publications

The Significance of the Leucocyte Concentrate in the Demonstration of Tumour Cells in the Blood

The Significance of the Leucocyte Concentrate in the Demonstration of Tumour Cells in the Blood

Circulating tumor cell technologies

Fluorescence Microscopy in Exfoliative Cytology

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