The different effects of type I and IV collagens on the survival and proliferation of cultured BSC-1 cells.

1989

Cell Struct. Funct.

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ABSTRACT. The effect of a tumor-promoting phorbol ester on spreading behavior was investigated to clarify the involvement of the interactions between cells and substratum in the maintenance of cell viability and the control of cell proliferation. BSC-1 cells did not spread and lost cell viability after a 24-h incubation in the absence of calf serum. Addition of calf serum initially induced radial spreading and then polarized spreading, with the formation on stress fibers and focal contact-like structure, and enhanced survival. Vitronectin also induced both radial spreading and polarized spreading, and enhanced cell survival. 12-O-Tetradecanoylphorbol-13-acetate (TPA) induced radial spreading with actin ribbons in the absence of serum. It improved the survival of cells attached to the substratum, but not in suspension. TPA suppressed polarized spreading, formation of stress fibers and of focal contact-like structure, and cell proliferation, in the presence of serum. Phorbol did not have any effect. These results suggest that enhancement of radial spreading and inhibition of polarized spreading of BSC-1 cells by TPA are closely related to the enhancement of cell survival and inhibition of cell growth.Interactions between cells and the substratum play a crucial role in the stimulative effects of serum on survival and growth of many lines of cultured cells (5,8,11,14,26). Serum contains various factors, such as spreading factors and growth factors, that are responsible for the potentiation of survival and growth of cultured cells. Adhesion of the cells to an adequate substratum is necessary for their growth in the presence of the serum (28). In contrast, certain substrata, such as type I collagen, suppress proliferation of BSC-1 cells (a continuous line of kidney cells derived from the African green monkey), while type IV collagen promotes the survival of BSC-1 cells in the absence of calf serum (25). It remains unclear how cell adhesion modulates the proliferation and survival of cells.Many studies using interference reflection microscopy have indicated that there are two types of adhesion of cells to the substratum (15, 16): focal contact and close contact. Focal contacts of fibroblasts with the substratum are considered to be closely associated with the functions of fibronectin and actin filaments (1, 2, 7, 10 , 27). Extracellular deposits of fibronectin are observed around the focal contacts (13,27). The close contacts seem to play a role in the regulation of cell motility (15 , 16).

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Modulation of survival and proliferation of BSC-1 cells through changes in spreading behavior caused by the tumor-promoting phorbol ester TPA.

1989

Cell Struct. Funct.

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“…Cultured cells require certain components of serum for their survival (6,7,13,14), for example, survival of BSC-1 cells is promoted by the addition of calf serum (23). Active components in the serum seem to be epidermal and fibroblast growth factors.…”

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confidence: 99%

“…Fibronectin improves resistance of cells to injury (25) and enhances proliferation of cultured cells (20,22). Type IV collagen enhances the survival of BSC-1 cells in the absence of calf serum (23). Attachment of cells to the substratum may also be necessary for the promotion of cell survival by growth factors.…”

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confidence: 99%

Survival of 3T3-L1 cells induced by fibroblast growth factor depends on cell density and adhesion to the substratum.

Yamada

1989

Cell Struct. Funct.

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ABSTRACT. The effects of cell contacts and the attachment of cells to the substratum on growth-factor-induced survival of 3T3-L1 cells were investigated to clarify their involvement in the maintenance of cell viability. When 3T3-L1 cells in low-density cultures or in high-density cultures were harvested with EDTA solution and cultured in the absence of calf serum, almost all cells from the low-density cultures lost viability 24 h later. However, about 15% of the cells harvested from high-density cultures survived for 24 h in the absence of calf serum. Addition of calf serum also enhanced the survival of cells from high-density cultures to a much greater extent than that of cells from low-density cultures. Addition of fibroblast growth factor enhanced the survival of cells, especially in the case of cells from high-density cultures. However, epidermal growth factor and platelet-dervied growth factor failed to enhance survival. Coating of culture dishes with vitronectin slightly enhanced cell survival. Addition of fibroblast growth factor markedly enhanced the survival of cells on the dishes coated with vitronectin or with fibronectin, but not on the dishes coated with heat-denatured bovine serum albumin. These results suggest that fibroblast growth factor promotes survival of 3T3-L1 cells, depending on cellto-cell contacts during prior culture and on the adhesion of cells to the substratum.

Survival of BSC-1 cells through the maintenance of cell volume brought about by epidermal growth factor depends on attachment to the substratum

1990

Experientia