The difference in size between single pronuclei after ICSI and after IVF

Igashira, C.; Otsuki, Junko; Furuhashi, Kohyu; Katada, Yuya; Sumimoto, T.; Kishi, Kanako; Matsuura, Motokazu; Mukai, Maira Mitsue; Sumi, C.; Tsuji, Yuta; Matsumoto, Yukiko; Kokeguchi, Shoji; Shiotani, Masahide

doi:10.1016/j.fertnstert.2016.07.058

Cited by 1 publication

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“…Time-lapse systems are not commonly used to analyse 1PN ICSI zygotes, and the few published studies that have employed this technique focus mainly on analyzing pronuclear formation. Studies that evaluated the potential of 1PN-derived embryos to reach the blastocyst stage are performed in traditional incubators or do not evaluate embryo kinetics (Yamaguchi et al, 2013;Igashira et al, 2016;Mateo et al, 2017;Araki et al, 2018). The novelty of our study lies in the kinetics of embryo development of 1PN ICSI zygotes, which were comprehensively analyzed from fertilization to blastocyst stage, and the identification of different morphokinetic behaviours that can be associated with the abnormal pronuclear pattern of 1PN-derived embryos.…”

Section: Discussionmentioning

confidence: 99%

“…Although it only represents 2-5% of intracytoplasmic sperm injection (ICSI) zygotes (Staessen and Van Steirteghem, 1997;Itoi et al, 2015;Bradley et al, 2017;Mateo et al, 2017), it is still interesting to study this pattern. Preliminary TL studies have assessed monopronucleated zygotes (1PN), generally focusing on the fertilization process and early development (Boada et al, 2012;Yamaguchi et al, 2013;Igashira et al, 2016;Tanaka et al, 2016;Araki et al, 2018). Using this technology, some authors have been able to detect a novel mechanism of 1PN formation involving the complete extrusion of female genetic material in a third polar body-like cell (Mio et al, 2014;Iwata and Mio, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Morphokinetics and in vitro developmental potential of monopronucleated ICSI zygotes until the blastocyst stage

Mateo

Vidal

Carrasco

et al. 2020

Zygote

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SummaryThe aim of this study was to provide a more comprehensive understanding of 1PN intracytoplasmic sperm injection (ICSI) zygotes. To achieve this objective, we assessed whether all 1PN-derived embryos showed a similar morphokinetic pattern, and if the morphokinetic behaviour of 1PN-derived embryos was comparable with that of 2PN-derived embryos. In total, 149 1PN ICSI zygotes (study group) and 195 2PN ICSI zygotes (control group) were included in the study. Embryo development potential was evaluated in terms of blastocyst rate. Morphokinetic parameters, including the pronucleus diameter and kinetics of in vitro development, were also analyzed. Embryos derived from 1PN ICSI zygotes showed impaired development compared with 2PN-derived embryos, with blastocyst rates of 28.9% and 67.2%, respectively. The diameter of the pronucleus of 1PN zygotes was larger than that of 2PN zygotes. When compared with 2PN-derived embryos, those derived from 1PN zygotes had a visible pronucleus for a shorter time, in addition to a longer syngamy time and slower kinetic behaviour from two to nine cells. When 1PN-derived blastocysts and 2PN-derived blastocysts were compared, the developmental kinetics were similar in both groups, except for a delayed and longer duration of the compaction phase in 1PN-derived embryos. In conclusion, monopronucleated ICSI zygotes present differences in developmental capacity and morphokinetic behaviour compared with 2PN ICSI zygotes, showing particular morphokinetic parameters related to pronucleus formation. Only the 1PN ICSI-derived embryos that reached the blastocyst stage have similar morphokinetic development to blastocysts from 2PN zygotes.

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Section: Discussionmentioning

confidence: 99%