The Difference in Repeatability of Automated Superficial Retinal Vessel Density according to the Measurement Area Using OCT Angiography

Abstract

Purpose. To evaluate the difference in the repeatability of automated superficial retinal vessel density and foveal avascular zone (FAZ) metrics according to the measurement area of optical coherence tomography angiography (OCTA). Methods. A total of 127 normal eyes from 127 healthy subjects were included. Macular angiography images were acquired from all subjects using the Zeiss Cirrus 5000 with AngioPlex™ OCTA software. Scans of 3 × 3 mm and 6 × 6 mm were each performed twice in a randomly arranged sequence. Vessel density (VD), perfusion density (PD), and FAZ metrics of the superficial capillary plexus were calculated automatically for all scans, and the repeatabilities for both scan patterns were assessed based on intraclass correlation (ICC), coefficient of variation (CV), and coefficient of repeatability (CR) parameters. The average measured values in the two scan patterns were also compared. Results. VD was significantly greater in the 3 × 3 mm scan than in the 6 × 6 mm scan according to all parameters, whereas PD was significantly less in the 3 × 3 mm scan than in the 6 × 6 mm scan. The ICCs for VDs in the central fovea were 0.826 and 0.741 for the 3 × 3 and 6 × 6 mm scans, respectively, and the CVs were 8.00% and 12.75%. For PDs, the ICCs were 0.839 and 0.762 and the CVs were 9.32% and 14.90%. The FAZ metrics in the 3 × 3 mm scan showed good repeatability with an ICC >0.75 and a CV <10.0%. However, all ICCs for the 6 × 6 mm scans were <0.75, and the CVs were all >10%. Conclusions. The 6 × 6 mm macular angiography scans resulted in lower repeatabilities than the 3 × 3 mm scans according to all OCTA parameters, particularly in the central fovea and FAZ metrics. The 3 × 3 mm scan was more suitable than the 6 × 6 mm scan for analyzing macular microvascular density and FAZ metrics.

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Assessment of Retinal Microvascular Alterations in Individuals With Amnestic and Nonamnestic Mild Cognitive Impairment Using Optical Coherence Tomography Angiography

“…Given that 3 × 3-mm OCTA scans may be more useful in identifying subtle macular microvascular changes, it is possible that the 3 × 3-mm OCTA scan is the preferred method for detecting novel biomarkers in amnestic and nonamnestic MCI patients. 25…”

Effect of Mydriatic Administration On Retinal Hemodynamics in Glaucoma: An Optical Coherence Tomography Angiography Study

“…However, good repeatability and reproducibility of FAZ area measurements performed with the AngioPlex software were shown in both 6×6 mm and 3×3 mm scan areas previously. 26 Furthermore, a comparative study found that macular OCTA 6×6 mm scans detect glaucomatous changes better than 3x3 mm scans. 27 The FAZ area was suggested as a good indicator of macular capillary health and linked to ischemic ocular disease progression.…”