The dietary flavonoid eupatilin attenuates <i>in vitro</i> lipid peroxidation and targets lipid profile in cancer HeLa cells

Rosa, Antonella; Isola, Raffaella; Pollastro, Federica; Caria, Paola; Appendino, Giovanni; Nieddu, Mariella

doi:10.1039/d0fo00777c

Cited by 13 publications

(87 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, in gastric cancer cells, eupatilin induces apoptosis by regulating apoptotic proteins such as BAX and BCL2 and inducing mitochondrial depolarization [8]. Eupatilin also induces apoptosis by mediating mitochondrial membrane depolarization in cervical cancer cells [18]. Similarly, we found that eupatilin can regulate the expression of the mitochondrial proteins BAK, BCL-xL, and cytochrome c, which are involved in apoptotic processes in colon cancer cells, as well as in mitochondrial depolarization.…”

Section: Discussionsupporting

confidence: 51%

Eupatilin Impacts on the Progression of Colon Cancer by Mitochondria Dysfunction and Oxidative Stress

et al. 2021

View full text Add to dashboard Cite

Colon cancer is one of the most frequently diagnosed cancer types. Some colon cancer cases resist standard anticancer drugs. Therefore, many studies have focused on developing therapeutic supplements using natural products with low side effects and broad physiological activity. Eupatilin is a flavonoid that is mainly extracted from artemisia and promotes apoptosis in numerous cancer types. However, since the current understanding of its physiological mechanisms on colon cancer cells is insufficient, we investigated how eupatilin affects the growth of two colon cancer cell lines, namely HCT116 and HT29. Our results showed that eupatilin inhibits cell viability and induces apoptosis accompanied by mitochondrial depolarization. It also induces oxidative stress in colon cancer cells and regulates the expression of proteins involved in the endoplasmic reticulum stress and autophagic process. Moreover, eupatilin may target the PI3K/AKT and mitogen-activated protein kinase (MAPK) signaling pathways in colon cancer cells. It also prevents colon cancer cell invasion. Furthermore, eupatilin has a synergistic effect with 5-fluorouracil (5-FU; a standard anticancer drug) on 5-FU-resistant HCT116 cells. These results suggest that eupatilin can be developed as an adjuvant to enhance traditional anticancer drugs in colon cancer.

show abstract

Section: Discussionsupporting

confidence: 51%

Eupatilin Impacts on the Progression of Colon Cancer by Mitochondria Dysfunction and Oxidative Stress

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Activation of SREBP-1 to synthesize lipids is thought to be required to maintain rapid proliferation of tumor cells [ 37 ]. It has been reported that lipogenesis also contributes to the resistance to cell death by altering membrane lipid composition and sensitive to lipid peroxidation [ 12 , 38 – 40 ]. In gefitinib-sensitive PC9 cells, gefitinib caused a decrease in lipogenesis and attenuated the expression of SREBP1, mSREBP1, FASN, and SCD.…”

Section: Discussionmentioning

confidence: 99%

Lipidomics reveals that sustained SREBP-1-dependent lipogenesis is a key mediator of gefitinib-acquired resistance in EGFR-mutant lung cancer

Zhang

Wang

et al. 2021

Cell Death Discov.

View full text Add to dashboard Cite

Patients with EGFR mutations in non-small cell lung cancer (NSCLC) have been greatly benefited from gefitinib, however, the therapeutic has failed due to the presence of acquired resistance. In this study, we show that gefitinib significantly induces downregulation of Sterol Regulator Element Binding (SREBP1) in therapy-sensitive cells. However, this was not observed in EGFR mutant NSCLC cells with acquired resistance. Lipidomics analysis showed that gefitinib could differently change the proportion of saturated phospholipids and unsaturated phospholipids in gefitinib-sensitive and acquired-resistant cells. Besides, levels of ROS and MDA were increased upon SREBP1 inhibition and even more upon gefitinib treatment. Importantly, inhibition of SREBP1 sensitizes EGFR-mutant therapy-resistant NSCLC to gefitinib both in vitro and in vivo models. These data suggest that sustained de novo lipogenesis through the maintenance of active SRBEP-1 is a key feature of acquired resistance to gefitinib in EGFR mutant lung cancer. Taken together, targeting SREBP1-induced lipogenesis is a promising approach to overcome acquired resistance to gefitinib in EGFR-mutant lung cancer.

show abstract

“…Apabila enzim tersebut dihambat, maka kadar kolesterol dapat menurun (Ekananda, 2015;Santosa, et al, 2020). Hasil ini sejalan dengan penelitian yang dilakukan oleh Rosa et al (2020) yang menyatakan bahwa pada tikus yang diberikan diet tinggi flavonoid, berupa beras merah, dapat menurunkan kadar kolesterol, LDL dan trigliserida serta peningkatan kadar HDL secara signifikan (Rosa, et al, 2020). Senyawa flavonoid akan berikatan dengan enzim HMG-KoA reduktase sehingga akan terbentuk asam mevalonat yang kemudian menyebabkan sintesis kolesterol terhenti (Zeka, et al, 2017).…”

Section: Pembahasanunclassified

Pengaruh Ekstrak Etanol Daun Rhizophora apiculata terhadap Kolesterol Total dan Trigliserida Rattus norvegicus Galur Sprague dawley yang Diinduksi Diet Tinggi Lemak

Mustofa

Adli²,

Wardani³

et al. 2022

View full text Add to dashboard Cite

The dietary flavonoid eupatilin attenuates in vitro lipid peroxidation and targets lipid profile in cancer HeLa cells

Abstract: Eupatilin, a dietary flavonoid, is an antioxidant agent against membrane lipid oxidative damage and induces cytotoxicity, apoptosis and abnormal mitosis, affecting lipid profile and mitochondrial potential in cancer HeLa cells.

Cited by 13 publications

References 49 publications

Eupatilin Impacts on the Progression of Colon Cancer by Mitochondria Dysfunction and Oxidative Stress

Eupatilin Impacts on the Progression of Colon Cancer by Mitochondria Dysfunction and Oxidative Stress

Lipidomics reveals that sustained SREBP-1-dependent lipogenesis is a key mediator of gefitinib-acquired resistance in EGFR-mutant lung cancer

Pengaruh Ekstrak Etanol Daun Rhizophora apiculata terhadap Kolesterol Total dan Trigliserida Rattus norvegicus Galur Sprague dawley yang Diinduksi Diet Tinggi Lemak

Contact Info

Product

Resources

About