The diagnostic value of pCEA, mCEA, AFP and CK19 in differentiating primary and metastatic carcinoma of liver

Background/Aim: Hepatocellular carcinoma (HCC) is one of the most aggressive cancer. MicroRNAs (miRNAs), are small noncoding regulatory RNAs, that function post-transcriptionally. MiRNA deregulation was observed in the development and progression of HCC. In this study we aimed to investigate the expression levels of four miRNA (mir-33a, mir-203b, mir361-3p and mir-424) in HCC patients in comparison to healthy individuals. Materials and methods: Venous blood samples were collected from both HCC patients and healthy individuals. In order to determine relative expression levels of mir-33a, mir-203b, mir361-3p and hsa-mir-424 in HCC patients using probe-based Quantitative Real Time PCR (qRT-PCR) is performed. The cycle threshold (Ct) results were analyzed according to the 2 −∆∆Ct method and statistical analyses was performed by IBM SPSS Statistics for Windows, version 15. Results: qRT-PCR analysis revealed that the expression level of mir-33a (fold change: 7.3 and p<0,001), mir-203b (fold change: 4.6 and p<0,001), mir361-3p (fold change: 5.1 and p<0,001) were down regulated compared to healthy individuals and mir-424 did not show any significant change between the HCC patients and controls. Conclusion: Our results indicated that mir-33a, mir-203b and mir-361-3p, may significantly contribute to tumor pathogenesis in HCC and would have potential to be used as a non-invasive biomarker for cancer therapy.

show abstract

“…of primary liver tumors [1,2]. Infection with Chronic Viral Hepatitis B and Hepatitis C are leading etiological factor for the developing HCC.…”

Section: Introductionmentioning

confidence: 99%

Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma

et al. 2021

View full text Add to dashboard Cite

show abstract

Assessment of circulating levels of microRNA-326, microRNA-424, and microRNA-511 as biomarkers for hepatocellular carcinoma in Egyptians

Youssef¹,

fiky²,

Nabeel³

et al. 2022

WJH

View full text Add to dashboard Cite

BACKGROUND Hepatocellular carcinoma (HCC) is the fifth most common cancer. Differential expression of microRNAs (miRNAs)-326, miRNA-424, and miRNA-511 has been associated with the diagnosis and prognosis of HCC in different populations. However, limited information is available regarding their expression in Egyptian HCC patients. AIM To assess the role of circulating miRNAs-326, miRNA-424, and miRNA-511 in Egyptian HCC patients. METHODS This prospective observational study included 70 HCC patients and 25 healthy controls. The circulating levels of these three miRNAs were evaluated by real-time PCR. Receiver operating characteristic curve analysis was used to test the diagnostic accuracy of microRNA expression levels. RESULTS All miRNAs were differentially expressed in HCC patients; miRNAs326 and miRNA-424 were upregulated, while miRNA-511 was downregulated. Both miRNA-326 and miRNA-424 showed sensitivity and specificity of 97%, 71.4%, and 52%, 60%, respectively, to differentiate HCC from controls. Moreover, miRNA-326 was associated with survival and could differentiate between Child grades (A vs B); miRNA-424 significantly differentiated early vs intermediate stages of HCC; while miRNA-511 was significantly correlated with response to modified Response Evaluation Criteria in Solid Tumors (mRECIST). CONCLUSION We conclude that miRNA-326, miRNA-424, and miRNA-511 have diagnostic and prognostic roles in Egyptian patients with hepatitis C virus-related HCC and should be considered for better disease management.

show abstract

The diagnostic value of pCEA, mCEA, AFP and CK19 in differentiating primary and metastatic carcinoma of liver

Abstract: Karaciğerin primer ve metastatik karsinomlarının ayrımında pCEA, mCEA, AFP ve CK19' un tanısal değeriThe diagnostic value of pCEA, mCEA, AFP and CK19 in differentiating primary and metastatic carcinoma of liver

Cited by 2 publications

References 4 publications

Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma

Role of mir-33a, mir-203b, mir361-3p, and mir-424 in hepatocellular carcinoma

Assessment of circulating levels of microRNA-326, microRNA-424, and microRNA-511 as biomarkers for hepatocellular carcinoma in Egyptians

Contact Info

Product

Resources

About