The diagnostic significance of circulating miRNAs and metabolite profiling in early prediction of breast cancer in Egyptian women

Abstract

Objective Breast cancer (BC) is one of the most commonly diagnosed solid malignancies in women worldwide. Purpose Finding new non-invasive circulating diagnostic biomarkers will facilitate the early prediction of BC and provide valuable insight into disease progression and response to therapy using a safe and more accessible approach available every inspection time. Therefore, our present study aimed to investigate expression patterns of potentially circulating biomarkers that can differentiate well between benign, malignant, and healthy subjects. Methods To achieve our target, quantitative analyses were performed for some circulating biomarkers which have a role in the proliferation and tumor growth, as well as, glutamic acid, and human epidermal growth receptor 2 (HER2) in blood samples of BC patients in comparison to healthy controls using qRT-PCR, liquid chromatography/mass spectrometry (LC/MS/MS), and ELISA. Results Our findings showed that the two miRNAs (miRNA-145, miRNA-382) were expressed at lower levels in BC sera than healthy control group, while miRNA-21 was expressed at higher levels in BC patients than control subjects. Area under ROC curves of BC samples revealed that AUC of miRNA-145, miRNA-382, miRNA-21, and glutamic acid was evaluated to equal 0.99, 1.00, 1.00 and 1.00, respectively. Besides, there was a significantly positive correlation between miRNA-145 and miRNA-382 (r = 0.737), and a highly significant positive correlation between miRNA-21 and glutamic acid (r = 0.385). Conclusion Based on our results, we conclude that the detection of serum miRNA-145, -382 and -21 as a panel along with glutamic acid, and circulating HER2 concentrations could be useful as a non-invasive diagnostic profiling for early prediction of breast cancer in Egyptian patients. It can provide an insight into disease progression, discriminate between malignancy and healthy control, and overcome the use limitations (low sensitivity and specificity, repeated risky exposure, and high cost) of other detecting tools, including mammography, magnetic resonance imaging, and ultrasound.

Circulating microRNA Panels for Detection of Liver Cancers and Liver-Metastasizing Primary Cancers

“…Some studies described discovery phases with methods of gene expression array [78,80,88], sequencing [79,86,87], or bioinformatics [81,83,85]. The studies were two-phased [80,82,83,[86][87][88][89][90] and three-phased [77][78][79]81,85].…”

“…For the detection of LC, the panels comprised from two to six miRNAs per panel, with the statistical significance of AUC from 0.8013 to 1 (Table 6). Although the AUC was 1, we have to acknowledge that the sample size was quite small, with only 30 NSCLC and 20 healthy control samples [89]. In LC, we have three panels with cohort sizes over 400 cases.…”

Dynamic expression of H19 and MALAT1 and their correlation with tumor progression biomarkers in a multistage hepatocarcinogenesis model

“…ncRNAs include microRNAs, circular RNAs, intronic RNAs, and long noncoding RNAs (lncRNAs). Many studies focused on the functional roles of miRNAs as diagnostic and prognostic biomarkers for various cancer types 12–15 . However, recent studies highlighted the critical importance of lncRNAs in regulating gene expression at the transcriptional or post‐transcriptional level 16–18 .…”

“…Many studies focused on the functional roles of miRNAs as diagnostic and prognostic biomarkers for various cancer types. [12][13][14][15] However, recent studies highlighted the critical importance of lncRNAs in regulating gene expression at the transcriptional or posttranscriptional level. [16][17][18] Numerous lncRNAs have been identified as oncogenes or tumor suppressors, with implications in cell proliferation, EMT, and angiogenesis, in addition to other cellular processes.…”

Restoring microRNA-34a overcomes acquired drug resistance and disease progression in human breast cancer cell lines via suppressing the ABCC1 gene

“…MicroRNAs (miRNAs) are very important gene expression regulators that have a vital role in controlling many cellular biological processes such as cell growth, proliferation, invasion, differentiation, adhesion, cell death, drug sensitivity/resistance, and cellular metabolic pathways. They are highly conserved naturally occurring small non-coding RNA molecules with a length of about 21–24 nucleotides which can bind partially to their complementary bases in the 3′- untranslated regions (3′UTR) of protein-coding mRNAs, resulting in mRNA translation inhibition or destabilization, leading to gene silencing at the post-transcriptional level [ 6 , 9 – 11 ].…”