The diagnostic quality of needle brain biopsy specimens obtained with different sampling methods – Experimental study

Trojanowski, Piotr; Jarosz, Bożena; Szczepanek, Dariusz

doi:10.1038/s41598-019-44622-4

Cited by 13 publications

(7 citation statements)

References 44 publications

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“…The diameter of the SPME probe is approximately 200 μm, which makes it thinner than a classical brain biopsy needle (ca. 1.8 mm) and enables the sampling of analytes without significantly disrupting the studied lesion or organ [ 11 , 12 , 20 , 22 ]. Apart from minimal invasiveness during sampling, SPME fibers offer a few other key features that are useful in tissue analysis.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Bogusiewicz

Kupcewicz

Goryńska

et al. 2022

IJMS

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The development of a fast and accurate intraoperative method that enables the differentiation and stratification of cancerous lesions is still a challenging problem in laboratory medicine. Therefore, it is important to find and optimize a simple and effective analytical method of enabling the selection of distinctive metabolites. This study aims to assess the usefulness of solid-phase microextraction (SPME) probes as a sampling method for the lipidomic analysis of brain tumors. To this end, SPME was applied to sample brain tumors immediately after excision, followed by lipidomic analysis via liquid chromatography-high resolution mass spectrometry (LC-HRMS). The results showed that long fibers were a good option for extracting analytes from an entire lesion to obtain an average lipidomic profile. Moreover, significant differences between tumors of different histological origin were observed. In-depth investigation of the glioma samples revealed that malignancy grade and isocitrate dehydrogenase (IDH) mutation status impact the lipidomic composition of the tumor, whereas 1p/19q co-deletion did not appear to alter the lipid profile. This first on-site lipidomic analysis of intact tumors proved that chemical biopsy with SPME is a promising tool for the simple and fast extraction of lipid markers in neurooncology.

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Section: Introductionmentioning

confidence: 99%

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Bogusiewicz

Kupcewicz

Goryńska

et al. 2022

IJMS

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“…On the other hand the use of a side-cutting needle has been reported more frequently in recent years, but this trend is not supported by scientific evidence, but only by some opinions [ 21 ]. We can only find a comparison of tissue collection methods in a very small study of AIDS patients [ 13 ] and of dead pig brains [ 25 ]. In meta-analyzes [ 9 , 11 , 15 ] and systemic review [ 22 ] the results were not compared depending on the method of tissue collection during stereotactic biopsies.…”

Section: Discussionmentioning

confidence: 99%

Endoscopic versus stereotactic biopsies of intracranial lesions involving the ventricles

et al. 2020

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Stereotactic biopsies of ventricular lesions may be less safe and less accurate than biopsies of superficial lesions. Accordingly, endoscopic biopsies have been increasingly used for these lesions. Except for pineal tumors, the literature lacks clear, reliable comparisons of these two methods. All 1581 adults undergoing brain tumor biopsy from 2007 to 2018 were retrospectively assessed. We selected 119 patients with intraventricular or paraventricular lesions considered suitable for both stereotactic and endoscopic biopsies. A total of 85 stereotactic and 38 endoscopic biopsies were performed. Extra procedures, including endoscopic third ventriculostomy and tumor cyst aspiration, were performed simultaneously in 5 stereotactic and 35 endoscopic cases. In 9 cases (5 stereotactic, 4 endoscopic), the biopsies were nondiagnostic (samples were nondiagnostic or the results differed from those obtained from the resected lesions). Three people died: 2 (1 stereotactic, 1 endoscopic) from delayed intraventricular bleeding and 1 (stereotactic) from brain edema. No permanent morbidity occurred. In 6 cases (all stereotactic), additional surgery was required for hydrocephalus within the first month postbiopsy. Rates of nondiagnostic biopsies, serious complications, and additional operations were not significantly different between groups. Mortality was higher after biopsy of lesions involving the ventricles, compared with intracranial lesions in any location (2.4% vs 0.3%, p = 0.016). Rates of nondiagnostic biopsies and complications were similar after endoscopic or stereotactic biopsies. Ventricular area biopsies were associated with higher mortality than biopsies in any brain area.

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“…QuPath v0.2.0 110 was used to detect areas of microhemorrhage and TUNEL expression. The imaged slice for mouse histological analysis was segmented into the tumor region of interest (ROI) that includes the tumor mass and extends 0.5 mm into its periphery, which is consistent with the safety objectives from previous studies 111 and the potential damage caused by the external and lumen diameters of a biopsy needle 112 , 113 . The parenchyma ROI was defined as the whole imaged slice without the tumor ROI.…”

Section: Methodsmentioning

confidence: 99%

Sonobiopsy for minimally invasive, spatiotemporally-controlled, and sensitive detection of glioblastoma-derived circulating tumor DNA

Pacia¹,

Yue²,

Xu³

et al. 2022

Theranostics

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Though surgical biopsies provide direct access to tissue for genomic characterization of brain cancer, they are invasive and pose significant clinical risks. Brain cancer management via blood-based liquid biopsies is a minimally invasive alternative; however, the blood-brain barrier (BBB) restricts the release of brain tumor-derived molecular biomarkers necessary for sensitive diagnosis. Methods: A mouse glioblastoma multiforme (GBM) model was used to demonstrate the capability of focused ultrasound (FUS)-enabled liquid biopsy (sonobiopsy) to improve the diagnostic sensitivity of brain tumor-specific genetic mutations compared with conventional blood-based liquid biopsy. Furthermore, a pig GBM model was developed to characterize the translational implications of sonobiopsy in humans. Magnetic resonance imaging (MRI)-guided FUS sonication was performed in mice and pigs to locally enhance the BBB permeability of the GBM tumor. Contrast-enhanced T 1 -weighted MR images were acquired to evaluate the BBB permeability change. Blood was collected immediately after FUS sonication. Droplet digital PCR was used to quantify the levels of brain tumor-specific genetic mutations in the circulating tumor DNA (ctDNA). Histological staining was performed to evaluate the potential for off-target tissue damage by sonobiopsy. Results: Sonobiopsy improved the detection sensitivity of EGFRvIII from 7.14% to 64.71% and TERT C228T from 14.29% to 45.83% in the mouse GBM model. It also improved the diagnostic sensitivity of EGFRvIII from 28.57% to 100% and TERT C228T from 42.86% to 71.43% in the porcine GBM model. Conclusion: Sonobiopsy disrupts the BBB at the spatially-targeted brain location, releases tumor-derived DNA into the blood circulation, and enables timely collection of ctDNA. Converging evidence from both mouse and pig GBM models strongly supports the clinical translation of sonobiopsy for the minimally invasive, spatiotemporally-controlled, and sensitive molecular characterization of brain cancer.

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The diagnostic quality of needle brain biopsy specimens obtained with different sampling methods – Experimental study

Cited by 13 publications

References 44 publications

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Endoscopic versus stereotactic biopsies of intracranial lesions involving the ventricles

Sonobiopsy for minimally invasive, spatiotemporally-controlled, and sensitive detection of glioblastoma-derived circulating tumor DNA

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