The Diagnostic Performance of Early Sjögren’s Syndrome Autoantibodies in Juvenile Sjögren’s Syndrome: The University of Florida Pediatric Cohort Study

Thatayatikom, Akaluck; Jun, Inyoung; Bhattacharyya, Indraneel; Berg, Kathleen M.; Lee, Yun Jong; Kim, Yoosik; Adewumi, Abimbola O.; Zhang, Weizhou; Thatayatikom, Sthorn; Shah, Ankit; Beal, Casey J.; Modica, Renee; Elder, Melissa E.; Cha, Seunghee

doi:10.3389/fimmu.2021.704193

Cited by 10 publications

(11 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Again, only six subjects with SSA− SjD were included in the SjD group, limiting the generalisability of these findings to this population. Additional studies have failed to show an increase of these antibodies in children 28. Aquaporin 5 antibodies are higher in subjects with SjD than subjects without SjD29 30 and correlate with FS,30 but the performance of the assay in subjects with SSA− SjD remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Novel autoantibodies help diagnose anti-SSA antibody negative Sjögren disease and predict abnormal labial salivary gland pathology

Parker,

Zheng,

Lasarev

et al. 2024

Ann Rheum Dis

View full text Add to dashboard Cite

ObjectivesSjögren disease (SjD) diagnosis often requires either positive anti-SSA antibodies or a labial salivary gland biopsy with a positive focus score (FS). One-third of patients with SjD lack anti-SSA antibodies (SSA−), requiring a positive FS for diagnosis. Our objective was to identify novel autoantibodies to diagnose ‘seronegative’ SjD.MethodsIgG binding to a high-density whole human peptidome array was quantified using sera from SSA− SjD cases and matched non-autoimmune controls. We identified the highest bound peptides using empirical Bayesian statistical filters, which we confirmed in an independent cohort comprising SSA− SjD (n=76), sicca-controls without autoimmunity (n=75) and autoimmune-feature controls (SjD features but not meeting SjD criteria; n=41). In this external validation, we used non-parametric methods for binding abundance and controlled false discovery rate in group comparisons. For predictive modelling, we used logistic regression, model selection methods and cross-validation to identify clinical and peptide variables that predict SSA− SjD and FS positivity.ResultsIgG against a peptide from D-aminoacyl-tRNA deacylase (DTD2) bound more in SSA− SjD than sicca-controls (p=0.004) and combined controls (sicca-controls and autoimmune-feature controls combined; p=0.003). IgG against peptides from retroelement silencing factor-1 and DTD2 were bound more in FS-positive than FS-negative participants (p=0.010; p=0.012). A predictive model incorporating clinical variables showed good discrimination between SjD versus control (area under the curve (AUC) 74%) and between FS-positive versus FS-negative (AUC 72%).ConclusionWe present novel autoantibodies in SSA− SjD that have good predictive value for SSA− SjD and FS positivity.

show abstract

Section: Discussionmentioning

confidence: 99%

Novel autoantibodies help diagnose anti-SSA antibody negative Sjögren disease and predict abnormal labial salivary gland pathology

Parker,

Zheng,

Lasarev

et al. 2024

Ann Rheum Dis

View full text Add to dashboard Cite

show abstract

“…Myung-Chul Kim 1,2,3,4,5 , Umasankar De 1,2 , Nicholas Borcherding 6 , Lei Wang 1,2 , Joon Paek 5,6 , Indraneel Bhattacharyya 5,7 , Qing Yu 8 , Ryan Kolb 1,2 , Theodore Drashansky 9 , Akaluck Thatayatikom 10,11 , Weizhou Zhang 1,2,11 & Seunghee Cha 5,7,11 Childhood Sjögren's disease represents critically unmet medical needs due to a complete lack of immunological and molecular characterizations. This study presents key immune cell subsets and their interactions in the periphery in childhood Sjögren's disease.…”

Section: Check For Updatesmentioning

confidence: 99%

Single-cell transcriptomics unveil profiles and interplay of immune subsets in rare autoimmune childhood Sjögren’s disease

Kim,

De,

Borcherding

et al. 2024

Commun Biol

Self Cite

View full text Add to dashboard Cite

Childhood Sjögren’s disease represents critically unmet medical needs due to a complete lack of immunological and molecular characterizations. This study presents key immune cell subsets and their interactions in the periphery in childhood Sjögren’s disease. Here we show that single-cell RNA sequencing identifies the subsets of IFN gene-enriched monocytes, CD4+ T effector memory, and XCL1+ NK cells as potential key players in childhood Sjögren’s disease, and especially in those with recurrent parotitis, which is the chief symptom prompting clinical visits from young children. A unique cluster of monocytes with type I and II IFN-related genes is identified in childhood Sjögren’s disease, compared to the age-matched control. In vitro regulatory T cell functional assay demonstrates intact functionality in childhood Sjögren’s disease in contrast to reduced suppression in adult Sjögren’s disease. Mapping this transcriptomic landscape and interplay of immune cell subsets will expedite the understanding of childhood Sjögren’s disease pathogenesis and set the foundation for precision medicine.

show abstract

“…There are various autoantibodies associated with Sjögren’s syndrome. These include anti-salivary protein 1 (SP1), anti-carbonic anhydrase II and IV, anti-parotid secretory protein (PSP), anti-Ro (SS-A) and anti-La (SS-B), rheumatoid factor, and ANA ( 41 , 42 ).…”

Section: Problems Caused By Autoantibodiesmentioning

confidence: 99%

Autoantibodies - enemies, and/or potential allies?

Murphy²,

Loscher³

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Autoantibodies are well known as potentially highly harmful antibodies which attack the host via binding to self-antigens, thus causing severe associated diseases and symptoms (e.g. autoimmune diseases). However, detection of autoantibodies to a range of disease-associated antigens has enabled their successful usage as important tools in disease diagnosis, prognosis and treatment. There are several advantages of using such autoantibodies. These include the capacity to measure their presence very early in disease development, their stability, which is often much better than their related antigen, and the capacity to use an array of such autoantibodies for enhanced diagnostics and to better predict prognosis. They may also possess capacity for utilization in therapy, in vivo. In this review both the positive and negative aspects of autoantibodies are critically assessed, including their role in autoimmune diseases, cancers and the global pandemic caused by COVID-19. Important issues related to their detection are also highlighted.

show abstract

The Diagnostic Performance of Early Sjögren’s Syndrome Autoantibodies in Juvenile Sjögren’s Syndrome: The University of Florida Pediatric Cohort Study

Cited by 10 publications

References 49 publications

Novel autoantibodies help diagnose anti-SSA antibody negative Sjögren disease and predict abnormal labial salivary gland pathology

Novel autoantibodies help diagnose anti-SSA antibody negative Sjögren disease and predict abnormal labial salivary gland pathology

Single-cell transcriptomics unveil profiles and interplay of immune subsets in rare autoimmune childhood Sjögren’s disease

Autoantibodies - enemies, and/or potential allies?

Contact Info

Product

Resources

About