The diagnostic efficiency of some serological tests for bovine brucellosis

Chappel, R.J.; McNaught, D. J.; Bourke, J. A.; Allan, G. S.

doi:10.1017/s0022172400024827

Cited by 15 publications

(3 citation statements)

References 7 publications

(8 reference statements)

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“…The highest dose (7.8 x 1010 organisms) was in the range of the dose recommended for calflhood vaccination. The reduced dose that had been used in adult cattle in field trials (7,18,19) and is permitted in whole-herd vaccination under specific conditions (23) is intermediate between the two reduced doses studied here, i.e., 5 x 109 and 9 x iOW organisms.…”

Section: Methodsmentioning

confidence: 94%

Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis

Jones¹,

Berman²,

Moreno³

et al. 1980

J Clin Microbiol

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A radial immunodiffusion (RID) test employing a polysaccharide antigen (poly B) was compared with tests currently used in the diagnosis of bovine brucellosis. Over 1,000 sera from vaccinated and infected cattle, all of which had been examined bacteriologically, were used to determine the sensitivity and specificity of the RID, card, Rivanol, and complement fixation tests. The RID test identified 90% of the cattle that were shedding Brucella in their milk. Although the complement fixation test was more sensitive, it was less specific than the RID test in cattle vaccinated as adults with Brucella abortus strain 19. A sensitive screening test, such as the card test, in combination with the RID test could be used in diagnostic laboratories, or even in the field, with little additional expense or technical expertise. An additional advantage is that the RID could be applied to sera from adult cattle as early as 2 months after vaccination, when postvaccinal agglutinins and complement-fixing antibodies may still be present. The indirect hemolytic test was used with some of the sera and was found to be a very sensitive test which could be useful in areas of low incidence but would not be practical for large-scale testing in adult-vaccinated herds.

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Section: Methodsmentioning

confidence: 94%

Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis

Jones¹,

Berman²,

Moreno³

et al. 1980

J Clin Microbiol

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“…High levels of serum IgG2 antibody can cause false negative reactions in the CFT (Plackett & Alton, 1975). A previous survey of sera from Victorian cattle indicated that this occurred frequently, affected sera being positive by the RIA and often also by the less sensitive IHLT (Chappel et al 1978). However, that study was made early in the eradication programme, and may have included many older infected cattle whose IgG2 levels are higher (Williams & Millar, 1978).…”

Section: Discussionmentioning

confidence: 99%

Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

Chappel

Hayes

1983

J. Hyg.

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Sera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with Brucella abortus strain 19 was compulsory. Of 4583 sera positive by the Rose Bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. These figures, and supporting evidence from the eradication programme, suggest that the radioimmunoassay may be a useful supplementary test in problem herds.

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“…Data from Standard Agglutination Tube (SAT) Buffered Brucella Antigen or card (CT) and Complement Fixation (CF) tests have been compared to determine agreement among tests, and suitability of serological * This study was supported in part by the Texas Agricultural Experiment Station, F. C. HECK AND OTHERS methods, to evaluate the health status of the animal or herd exposed to B. abortus (Alton et at. 1975;Chappel et al 1978;Morgan, MacKinnon & Cullen, 1969;Morgan & Richards, 1974;Nicoletti, 1967;Rose & Roepke, 1957). Most cattle infected with B. abortus, except those which are recently infected, can be identified by the SAT test (Davies, 1971).…”

Section: Introductionmentioning

confidence: 99%

Comparison of serological methods for the detection of B. abortus antibodies in sera from vaccinated and non-vaccinated cattle

Heck

Williams

Crawford

et al. 1979

J. Hyg.

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SUMMARYA total of 4551 sera from 863 Strain 19 vaccinated and non-vaccinated adult cattle, independent of disease status, were tested by five serological methods to detect the presence of antibodies to B. abortus. Results from Standard Agglutination Tube (SAT), Buffered Brucella Antigen or card (CT), Complement Fixation (CF), Enzyme Linked Immunosorbent Assay (ELISA) and Rivanol (Riv) methods were compared.There was a 95 % probability for agreement among CT negative sera, between serological methods, for all groups of vaccinated and non-vaccinated cattle. The agreement between tests with Riv Positive sera, excluding the calfhood and adult vaccinated group tested by the CF method, was 91-100 %. The probability of a serum which was serologically negative by other methods being Riv negative was 98 %. The usefulness of serological results from Riv (> 1/50) tests for classifying the reactor status of cattle are of doubtful supplemental value to confirm card test positive results.Vaccination history is an important consideration when evaluating serological data on cattle sera particularly from SAT and CF methods.

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The diagnostic efficiency of some serological tests for bovine brucellosis

Cited by 15 publications

References 7 publications

Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis

Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis

Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

Comparison of serological methods for the detection of B. abortus antibodies in sera from vaccinated and non-vaccinated cattle

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