The Diagnostic Accuracy of Tests for Latent Tuberculosis Infection in Hemodialysis Patients

Ferguson, Thomas; Tangri, Navdeep; Macdonald, Kerry; Hiebert, Brett; Rigatto, Claudio; Sood, Manish M.; Shaw, Souradet Y.; Lerner, Blake; Xu, Yang; Mahmud, Salaheddin M.; Komenda, Paul

doi:10.1097/tp.0000000000000451

Cited by 51 publications

(63 citation statements)

References 30 publications

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“…This includes those who are HIV infected, where test sensitivity decreases with loss of CD4 T cell counts (30,31), and patients with end stage renal disease (32). Unfortunately, it is these same groups that have the highest risk for LTBI reactivation and require accurate testing and treatment.…”

Section: Ltbi Diagnosticsmentioning

confidence: 99%

Latent tuberculosis infection: An overview

Kiazyk¹,

Ball²

2017

Canada Communicable Disease Report

152

109

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Latent tuberculosis infection (LTBI) is defined as a state of persistent immune response to stimulation by Mycobacterium tuberculosis antigens without evidence of clinically manifested active tuberculosis (TB) disease. Individuals with LTBI represent a reservoir for active TB cases. The detection and management of LTBI is now a key component of the World Health Organization's End TB Strategy and the Government of Canada's federal framework for action on TB prevention and control. This is because people with LTBI can progress to active TB or undergo reactivation, a risk that is greatly increased in those with immunocompromising conditions. This overview provides a summary of LTBI and reactivation risk, as well as the recent advances in the diagnosis and treatment of LTBI.

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Section: Ltbi Diagnosticsmentioning

confidence: 99%

Latent tuberculosis infection: An overview

Kiazyk¹,

Ball²

2017

Canada Communicable Disease Report

152

109

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“…It is important to exclude tuberculosis in Indian patients with CD prior to institution of immunosuppressive treatment, since tuberculosis is endemic in the country. There is some controversy around the tests to use when screening for latent tuberculosis in immunosuppressed individuals, with studies suggesting both that interferon gamma release assays (IGRA) are less sensitive [112] or more sensitive [113] than tuberculin skin test (TST) in immunosuppressed individuals. It is important to remain aware that tuberculosis may get activated in CD patients on immunosuppressant treatment and to take appropriate steps to monitor them when necessary.…”

Section: Infliximab and Other Anti-tumor Necrosis Factor Antibodies Amentioning

confidence: 99%

Indian Society of Gastroenterology consensus statements on Crohn’s disease in India

Ramakrishna

Makharia

Ahuja

et al. 2015

Indian J Gastroenterol

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In 2012, the Indian Society of Gastroenterology's Task Force on Inflammatory Bowel Diseases undertook an exercise to produce consensus statements on Crohn's disease (CD). This consensus, produced through a modified Delphi process, reflects our current recommendations for the diagnosis and management of CD in India. The consensus statements are intended to serve as a reference point for teaching, clinical practice, and research in India.

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“…As shown in Figure 4 and Table 2, all patients responded to one or more immunogenic peptides, the percentage of responding patients varying between 45% and 73%. The percentages of TB patients responding to MPT63 [5][6][7][8][9][10][11][12][13][14] , MPT63 [10][11][12][13][14][15][16][17][18][19] , MPT63 [18][19][20][21][22][23][24][25][26] , MPT63 [20][21][22][23][24][25][26][27][28] and MPT63 [29][30][31][32][33][34][35][36][37] were 63%, 73%, 45%, 60% and 68%, respectively. The combined diagnostic sensitivity of these five immunogenic peptides was 93%.…”

Section: Sensitivity Of High-affinity Peptides In Tuberculosis Diagnosismentioning

confidence: 99%

“…The combined diagnostic sensitivity of these five immunogenic peptides was 93%. Patients responded strongly to MPT63 [5][6][7][8][9][10][11][12][13][14] , the frequency (median response and interquartile range per 2.5 Â 10 5 PBMCs) of peptide-specific IFN-g-secreting cells for all responding patients being 48 (8-145). The frequencies (median response and interquartile range) for the remaining four peptides were 35 (8-145), 33 (11-135), 28 (9-57) and 31 (10-85), respectively.…”

Section: Sensitivity Of High-affinity Peptides In Tuberculosis Diagnosismentioning

confidence: 99%

“…Currently, a commercialized ELISPOT assay (T-SPOT.TB assay) that utilizes TB-specific immunogenic antigens the (ESAT-6 and CFP-10) has been used for clinical diagnosis of latent and active TB (especially active pulmonary TB) (7)(8)(9). Although the diagnostic sensitivity of the T-SPOT.TB assay is higher than that of TST, it is still not high enough to rule out TB (10).…”

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confidence: 99%

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The diagnostic potential of MPT63‐derived HLA‐A*0201‐restricted CD8⁺T‐cell epitopes for active pulmonary tuberculosis

Duan

Jia

et al. 2015

Microbiology and Immunology

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MPT63 protein is found only in Mycobacterium tuberculosis complex, including M. tuberculosis and M. bovis. Detection of MPT63-specific IFN-g-secreting T cells could be useful for the diagnosis of tuberculosis (TB) diseases. In the present study, the HLA-A Ã 0201 restriction of ten predicted MPT63-derived CD8 + T-cell epitopes was assessed on the basis of T2 cell line and HLA-A Ã 0201 transgenic mice. The diagnostic potential of immunogenic peptides in active pulmonary TB patients was evaluated using an IFN-g enzyme-linked immunospot assay. It was found that five peptides bound to HLA-A Ã 0201 with high affinity, whereas the remaining peptides exhibited low affinity for HLA-A Ã 0201. Five immunogenic peptides (MPT63 18-26 , MPT63 29-37 , MPT63 20-28 , MPT63 5-14 and MPT63 [10][11][12][13][14][15][16][17][18][19] ) elicited large numbers of cytotoxic IFN-g-secreting T cells in HLA-A Ã 0201 transgenic mice. Each of the five immunogenic peptides was recognized by peripheral blood mononuclear cells from 45% to 73% of 40 HLA-A Ã 0201 positive TB patients. The total diagnostic sensitivity of the five immunogenic peptides was higher than that of a T-SPOT.TB assay (based on ESAT-6 and CFP-10) (93% versus 90%). It is noticeable that the diagnostic sensitivity of the combination of five immunogenic peptides and T-SPOT.TB assay reached 100%. These MPT63-derived HLA-A Ã 0201-restricted CD8 + T-cell epitopes would likely contribute to the immunological diagnosis of M. tuberculosis infection and may provide the components for designing an effective TB vaccine. Key words CD8+ T-cell epitope, HLA-A Ã 0201, MPT63, Mycobacterium tuberculosis.Tuberculosis, which is caused by the intracellular pathogen M. tuberculosis, is still a major global health problem, especially in developing countries (1). M. tuberculosis can infect all kinds of tissues or organs (especially lung) and causes TB-related diseases. Although the BCG vaccine is used worldwide, it is only currently effective against severe childhood forms of TB, its efficacy in adults being controversial (2). The increasing emergence of multidrug-resistant strains of M. tuberculosis has exacerbated the issue of TB. Currently, fast and precise diagnosis of M. tuberculosis infection is critical for the treatment of TB-related diseases. TSTs and detection of acid-fast bacilli in sputum samples are the conventional methods for List of Abbreviations: aa, amino acid; BCG, M. bovis Bacilli Calmette-Gu erin; CFP-10, 10 kDa culture filtrate antigen; ELISPOT, enzyme-linked immunospot; ESAT-6, 6 kDa early secretory antigenic target; E:T ratio, effector to target cell ratio; FI, fluorescence index; LDH, lactate dehydrogenase; LNC, lymph node cell; MFI, mean fluorescence intensity; PBMC, peripheral blood mononuclear cell; PPD, purified protein derivative; SFC, spot-forming cell; SMC, splenic mononuclear cell; TB, tuberculosis; TST, tuberculin skin test.

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The Diagnostic Accuracy of Tests for Latent Tuberculosis Infection in Hemodialysis Patients

Cited by 51 publications

References 30 publications

Latent tuberculosis infection: An overview

Latent tuberculosis infection: An overview

Indian Society of Gastroenterology consensus statements on Crohn’s disease in India

The diagnostic potential of MPT63‐derived HLA‐A*0201‐restricted CD8⁺T‐cell epitopes for active pulmonary tuberculosis

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The Diagnostic Accuracy of Tests for Latent Tuberculosis Infection in Hemodialysis Patients

Cited by 51 publications

References 30 publications

Latent tuberculosis infection: An overview

Latent tuberculosis infection: An overview

Indian Society of Gastroenterology consensus statements on Crohn’s disease in India

The diagnostic potential of MPT63‐derived HLA‐A*0201‐restricted CD8+T‐cell epitopes for active pulmonary tuberculosis

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Resources

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The diagnostic potential of MPT63‐derived HLA‐A*0201‐restricted CD8⁺T‐cell epitopes for active pulmonary tuberculosis