The Diagnosis of Bovine Tuberculosis

Kriek, N.P.J.; Areda, Demelash B.; Dibaba, Asseged B.

doi:10.1007/978-3-030-18690-6_9

Cited by 2 publications

(2 citation statements)

References 178 publications

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“…In infected animals, in an early pre‐granuloma forming status, in which infected tissues are macroscopically normal, the presence of small non‐homogeneous bacterial loads within the tissue together with the random choice of a small piece of tissue or the presence of inhibitory substances due to the lytic tissue material is conditioning factors that can explain the existence of false‐negative results (Fell et al., 2016). A more controversial interpretation is the possibility that part of the positive reactors to the SIT test are false positives due to the previous sensitization by other mycobacteria, either naturally (such as due to the presence of PTB or other mycobacterial infections) or artificially (effect of vaccination) (Chartier et al., 2012; Kriek, Areda, & Dibaba, 2019).…”

Section: Discussionmentioning

confidence: 99%

Influence of vaccination against paratuberculosis on the diagnosis of caprine tuberculosis during official eradication programmes in Castilla y León (Spain)

Espinosa

Fernández

Royo

et al. 2020

Transbounding Emerging Dis

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The information generated from the official eradication programmes of caprine tuberculosis (TB) in Castilla y León, Spain, during 2018, has been used to assess the effect of vaccination against paratuberculosis (PTB) and the presence of this infection, on the single intradermal tuberculin (SIT) test results. Data from 121,665 goats belonging to 1936 different herds were analysed using generalized linear models. An epidemiological survey was conducted to know the herd immunization status against PTB and the date of last vaccination. All SIT test-positive animals were further investigated in order to confirm the diagnosis of TB, through bacterial culture, and PTB, by histopathological and qPCR analyses. SIT positivity was found in 39 (2.01%) herds and 507 (0.41%) goats. TB was confirmed by M. caprae or M. bovis isolation in 10 (0.51%) herds and 46 (0.038%) goats. PTB was diagnosed in 13 (33.33%) and 55 (10.84%) of the SIT test-positive herds and goats, respectively. Vaccination against PTB showed a significant influence on the results of the SIT test at herd level, with higher positivity detected among those herds vaccinated. However, this effect was not observed when the total number of animals was considered, where the highest positivity was found in unvaccinated goats. The time elapsed between vaccination and SIT test performance also influenced the results. The strongest effect was found when less than eight months elapsed between performing both activities, and to a lesser extent between 8 and 12 months. Conversely, no positive herds or animals were found when the time elapsed was higher than one year. No significant effect of the presence of PTB was observed. These findings demonstrate that the use of PTB vaccine does not result in false positives to a SIT test at individual level, provided that the time elapsed between the performance of both practices is higher than 12 months.

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Section: Discussionmentioning

confidence: 99%

Influence of vaccination against paratuberculosis on the diagnosis of caprine tuberculosis during official eradication programmes in Castilla y León (Spain)

Espinosa

Fernández

Royo

et al. 2020

Transbounding Emerging Dis

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“…Tis is not a surprise because no lesions resembling M. bovis infection were visible on any of the samples processed. Tuberculosislike lesions are an indicator of the presence of tuberculosis [21,22]. According to Botha et al [23], tuberculosis is a slow and progressive disease that remains asymptomatic for an extended period until the advanced stages of infection when lesions appear.…”

Section: Discussionmentioning

confidence: 99%

Absence of Tuberculosis-Causing Mycobacteria from Slaughtered Livestock Tissues and Environmental Samples, Gauteng Province, South Africa

Mareledwane,

Adesiyun,

Hlokwe

2024

International Journal of Microbiology

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Mycobacterium tuberculosis complex (MTBC) is a group of bacteria responsible for causing tuberculosis in animals and humans. In South Africa (S.A), slaughterhouses are registered by the government and closely inspected and audited for hygienic slaughter practices. Meat inspection to detect lesions has been used for passive surveillance, monitoring, and diagnosis of the disease status. Information on the current status of bovine tuberculosis (bTB) in livestock in the country is limited. Hence, we investigated the occurrence of Mycobacterium spp. in the tissues of slaughtered livestock and environmental samples in abattoirs in Gauteng province of South Africa (S.A). The cross-sectional study employing random sampling from cattle, pigs, and sheep (with the collection of liver, lung, spleen, and different lymph nodes) irrespective of lesions was carried out in 19 red meat abattoirs. Five hundred animals were sampled, comprising cattle (n = 369), pigs (n = 90), and sheep (n = 41). Additionally, 19 environmental samples were collected from feedlots, or where animals drink water while awaiting slaughter, to identify mycobacterial species using culture, acid-fast bacteria staining, and polymerase chain reaction (PCR). The Chi-square and Fisher’s Exact tests were used to detect statistically significant differences in the frequency of detection of Mycobacterium spp. according to the variables investigated (types of tissues, livestock, abattoirs, etc.). The PCR assays detected no MTBC complex species DNA in the bacterial isolates from cattle (n = 32). Sequence analysis (16S rDNA) of the isolates from eight cattle confirmed only two species, namely Mycobacterium colombiense (99.81% identity) and Mycobacterium simiae (99.42% identity). The remaining isolates were identified as members of the Actinomadura species. From the environmental samples, bacterial isolation was made from three samples, and two could only be identified up to the genus level (Mycobacterium species) while the remaining isolate was identified as Mycobacterium senuense (99.22% identity). The study revealed the absence of bovine tuberculosis-causing pathogens in red meat abattoirs of the Gauteng province. Although non-tuberculous Mycobacteria have been implicated as potentially causing tuberculosis-like diseases in livestock, their occurrence in the current study was found to be low, but the potential to cause disease cannot be ignored.

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The Diagnosis of Bovine Tuberculosis

Cited by 2 publications

References 178 publications

Influence of vaccination against paratuberculosis on the diagnosis of caprine tuberculosis during official eradication programmes in Castilla y León (Spain)

Influence of vaccination against paratuberculosis on the diagnosis of caprine tuberculosis during official eradication programmes in Castilla y León (Spain)

Absence of Tuberculosis-Causing Mycobacteria from Slaughtered Livestock Tissues and Environmental Samples, Gauteng Province, South Africa

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