The diagnosis and successful replication of a clinical case of Duck Spleen Necrosis Disease: An experimental co-infection of an emerging unique reovirus and Salmonella indiana reveals the roles of each of the pathogens

Wang, Weiwei; JingZhen, Liang; Shi, Mengya; Huang, Yu; Zhang, Yan; Zhao, Zengzhi; Wang, Min; Li, Min; Mo, Meilan; Wei, Tianchao; Huang, Teng; He, Xiumiao; Wei, Ping

doi:10.1016/j.vetmic.2020.108723

Cited by 18 publications

(19 citation statements)

References 40 publications

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“…Importantly, Wang et al recently report that the emerging NDRV XT18 has extensive tissue tropism and could cause severe damage to the immune organs [3,8]. Furthermore, the NDRV co-infection with other pathogens can result in serious duck diseases [4]. At present, with the frequent trade of agricultural products between countries or regions, the possibility of virus transmission is greatly increased.…”

Section: Discussionmentioning

confidence: 99%

“…All the ducks used in the study were euthanized using the carbon dioxide method. The extraction of total RNA/DNA as noted in our previous description [4].…”

Section: Methodsmentioning

confidence: 99%

“…A recombinant plasmid used in the study was constructed by using the S3 gene of a Guangxi NDRV reference strain DRV/GX-Y7/2018/China (GenBank ID: MN747004-MN747013). The target gene was ampli ed, puri ed and cloned as the descriptions by our group have described previously [4]. Plasmid DNA was puri ed using the…”

Section: Recombinant Plasmid Constructionmentioning

confidence: 99%

“…Novel duck reovirus (NDRV), an avian Orthoreovirus, is an emerging important viral pathogen causing Duck Spleen Necrosis Disease (DSND) and infecting a variety of duck species recently [1][2][3][4]. The emerging NDRV can cause severe damage to the immune organs, and co-infected with Salmonella spp.…”

Section: Introductionmentioning

confidence: 99%

“…The emerging NDRV can cause severe damage to the immune organs, and co-infected with Salmonella spp. can greatly enhance its pathogenesis and further raise the risk of missed and/or mistaken diagnoses [4]. Compared with all previously reported duck reovirus (DRV) infections, NDRV infection exhibits higher virulence and widely host species [5] and the viral S3 gene have been used to distinguish NDRV from the Muscovy duck reovirus (MDRV) or broiler/layer-origin reovirus (ARV) [6,7].…”

Section: Introductionmentioning

confidence: 99%

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Rapid and Visual Detection of the Emerging Novel Duck Reovirus by Using a Specific and Sensitive Reverse Transcription Recombinase Polymerase Amplification Method

Wang

Zhang

Huang

et al. 2020

Preprint

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Background Duck spleen necrosis disease (DSND) caused by Novel Duck Reovirus (NDRV), is an emerging infectious disease that causes severely threaten to duck industry. Currently, the popular conventional PCR technique for detecting NDRV is time consuming. So, it is essential to develop a rapid and accurate molecular diagnosis techniques of viral pathogens for the purpose to prevent further disease transmission or outbreaks. Recombinase polymerase Amplification (RPA) is a new generation of simple, rapid and cost-effective molecular diagnosis technology, which has been applied to the molecular detection of various pathogens. Methods In our study, a simple, rapid and reliable detection method was developed target NDRV by an isothermal reverse transcription-recombinase polymerase amplification (RT-RPA). The RT-RPA primers were designed based on the S3 gene of NDRV, and a series of other waterfowl-origin pathogens were detected by RT-RPA. A total of 20 field and experimental infected samples were tested by RT-RPA and compared with the results of conventional RT-PCR and quantitative RT-PCR simultaneously. Results The RT-RPA method proved to be repeatable and could detect as little as 3.48 × 10− 6 ng/µl of the standard plasmid DNA inserted with the viral S3 gene. This was a 10 × higher sensitivity rate than that of conventional RT-PCR. The major advantage of this RT-RPA method is that it could be performed as an isothermal reaction at 37 ℃ and completed within 20 min. In addition, no cross-reactivity was detected with other waterfowl-origin viruses. Also, the amplified products could be visualized faster, without the gel electrophoresis, by adding the SYBR Green I and observing them under an ultraviolet light. Conclusions This newly developed RT-RPA method offers a simple, rapid and accurate for rapid detection of NDRV, which especially useful in on-site facilities and resource-limited areas.

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Section: Discussionmentioning

confidence: 99%

“…All the ducks used in the study were euthanized using the carbon dioxide method. The extraction of total RNA/DNA as noted in our previous description [4].…”

Section: Methodsmentioning

confidence: 99%

Section: Recombinant Plasmid Constructionmentioning

confidence: 99%