The developmental transcriptome of the synanthropic fly Chrysomya megacephala and insights into olfactory proteins

Wang, Xiaoyun; Xiong, Momiao; Lei, Chaoliang; Zhu, Fen

doi:10.1186/s12864-014-1200-y

Cited by 38 publications

(27 citation statements)

References 45 publications

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“…Briefly, mRNA was purified from 3 μg total RNA per sample using oligo (dT) magnetic beads. Transcriptome libraries were generated using standard protocols as previously reported [ 27 , 28 ]. The serial numbers were as follows: sml males (with the serial number as sml _M), sml females (with the serial number as sml _F), Dazao males (with the serial number as DZ_M) and Dazao females (with the serial number as DZ_F).…”

Section: Methodsmentioning

confidence: 99%

Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes

Tong

et al. 2016

PLoS ONE

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As a source of insect polymorphism, melanism plays an important role in ecological adaption and usually endows advantageous phenotypic-effects on insects. However, due to the mechanistic diversity, there are knowledge gaps in the molecular mechanisms underlying melanism and the related phenotypes. In silk moths, a recessive melanic mutant (sex-controlled melanism, sml) strain exhibits extended adult longevity. We took a transcriptome approach to perform a comparative analysis between this sml strain and a wild-type strain (Dazao). Our analysis resulted in the identification of 59 unique differentially expressed genes in the melanic mutant. Two key genes (laccase2 and yellow) involved in melanin formation were significantly up-regulated in melanic individuals. The laccase2 B-type isoform (BGIBMGA006746) was found to likely participate in the silkworm cuticular melanism process at late pupal stage. Moreover, we discovered 22 cuticular protein encoding genes with the possible function in melanin transport and/or maintenance. Based on our findings, we presume that the longer survival of the melanic sml male moths might be associated with the enhanced antioxidant defense systems and a reduction in the insulin/IGF-1 signaling pathway (IIS). These findings will facilitate the understanding of the molecular basis underlying melanism and the derived phenotypic-effects.

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Section: Methodsmentioning

confidence: 99%

Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes

Tong

et al. 2016

PLoS ONE

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“…Additionally, Hsp70 might be a potential reference gene in some experimental conditions. The results of this study have been used in gene expressions in the development and tissues of C. megacephala [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptomes of C. megacephala provided a great quantity of sequence information [ 20 , 21 ]. The developmental gene bicoid has been used to identify species of forensically important blowflies, avoiding confusion in morphological parameters (Diptera: calliphoridae) [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Reference gene stability of a synanthropic fly, Chrysomya megacephala

et al. 2015

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BackgroundStable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qPCR). A synanthropic fly, Chrysomya megacephala, is a well known medical vector and forensic indicator. Unfortunately, previous studies did not look at the stability of reference genes used in C. megacephala.ResultsIn this study, the expression level of Actin, ribosomal protein L8 (Rpl8), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1α (EF1), α-tubulin (α-TUB), β-tubulin (β-TUB), TATA binding box (TBP), 18S rRNA (18S) and ribosomal protein S7 (Rps7) were evaluated for their stability using online software RefFinder, which combines the normal software of the ΔCt method, BestKeeper, Normfinder, and geNorm. Moreover the number of suitable reference gene pairs was also suggested by Excel-based geNorm. The expression levels of these reference genes were evaluated under different experimental conditions with special perspectives of forensic applications: developmental stages (eggs, first, second and third instar larvae, pupae and adults); food sources of larvae (pork, fish and chicken); feeding larvae with drugs (untreated control, Estazolam and Marvelon); feeding larvae with heavy metals (untreated control, cadmium and zinc); tissues of adults (head, thorax, abdomen, legs and wings). According to RefFinder, EF1 was the most suitable reference gene of developmental stages, food and tissues; 18S and GAPDH were the most suitable reference genes for drugs and heavy metals, respectively, which could be widely used for quantification of target gene expression with qPCR in C. megacephala. Suitable reference gene pairs were also suggested by geNorm.ConclusionThis fundamental but vital work should facilitate the gene studies of related biological processes and deepen the understanding in physiology, toxicology, and especially medical and forensic entomology of C. megacephala.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-015-1175-9) contains supplementary material, which is available to authorized users.

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“…Publication Ch. megacephala SRR620248 and SRR1663113 49,50 Co. hominivorax SRP044071 51 Figure 1. Assembly completeness.…”

Section: Species Ncbi Accessionmentioning

confidence: 99%

Functional genomics of the parasitic habits of blowflies and botflies

Cardoso

Deszo

Torres

2019

Preprint

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Infestation by dipterous larvaemyiasis -is a major problem for livestock industries worldwide and can cause severe economic losses. The Oestroidea superfamily is an interesting model to study the evolution of myiasis-causing flies because of the diversity of parasitism strategies among closely-related species. These flies are saprophagous, obligate parasites, or facultative parasites and can be subdivided into cutaneous, nasopharyngeal, traumatic, and furuncular. We expect that closely-related species have genetic differences that lead to the diverse parasitic strategies. To identify genes with such differences, we used gene expression and coding sequence data from five species (Cochliomyia hominivorax, Chrysomya megacephala, Lucilia cuprina, Dermatobia hominis, and Oestrus ovis). We tested whether 1,287 orthologs have different expression and evolutionary constraints under different scenarios. We found two up-regulated genes; one in species causing cutaneous myiasis that is involved in iron transportation/metabolization (ferritin), and another in species causing traumatic myiasis that responds to reduced oxygen levels (anoxia up-regulated-like). Our evolutionary analysis showed a similar result. In the Ch. hominivorax branch, we found one gene with the same function as ferritin that may be evolving under positive selection, spook. This is the first step towards understanding origins and evolution of parasitic strategy diversity in Oestroidea.

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The developmental transcriptome of the synanthropic fly Chrysomya megacephala and insights into olfactory proteins

Cited by 38 publications

References 45 publications

Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes

Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes

Reference gene stability of a synanthropic fly, Chrysomya megacephala

Functional genomics of the parasitic habits of blowflies and botflies

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