The developmental stage and cell type dependent phosphorylation of eNOS in murine enteric mucosa and myenteric plexus

Korkmaz, Hatice Ayça Ata; Bloch, Wilhelm; Bölck, Birgit; Labbé, D.; Addicks, Klaus; Arnhold, Stefan

doi:10.1007/s10735-007-9091-8

Cited by 4 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our data on the expression of iNOS in myenteric neurons are at least qualitatively confirmatory, as expression of iNOS has been observed (but not quantified) earlier in myenteric neurons in mouse 20,21 and rat. 18 Previous studies have reported on the expression of eNOS in submucosal neurons of the human and porcine jejunum 47 and in enteric neurons of the mouse colon 20,21,48 and rat intestine. 49 The present results obtained with immunohistochemistry and also at the mRNA level indicate that the proportion of eNOS-expressing neurons in the murine jejunum (<0.5%) is very small compared to the proportions of nNOS-and iNOS-expressing myenteric neurons.…”

Section: Myenteric Neurons Of Control Animals Express Nnos and Inosmentioning

confidence: 99%

Chronic alcohol consumption induces an overproduction of NO by nNOS- and iNOS-expressing myenteric neurons in the murine small intestine

Bagyánszki

Torfs

Krecsmarik

et al. 2011

Neurogastroenterology &Amp; Motility

View full text Add to dashboard Cite

show abstract

Section: Myenteric Neurons Of Control Animals Express Nnos and Inosmentioning

confidence: 99%

Chronic alcohol consumption induces an overproduction of NO by nNOS- and iNOS-expressing myenteric neurons in the murine small intestine

Bagyánszki

Torfs

Krecsmarik

et al. 2011

Neurogastroenterology &Amp; Motility

View full text Add to dashboard Cite

show abstract

“…The intensity of immunostaining is reported as the mean of measured cell gray value minus background gray value, which was detected at a cell‐free area of the slide (Korkmaz et al. ).…”

Section: Methodsmentioning

confidence: 99%

The red‐vine‐leaf extract AS195 increases nitric oxide synthase–dependent nitric oxide generation and decreases oxidative stress in endothelial and red blood cells

Grau

Bölck

Bizjak

et al. 2016

Pharmacology Res & Perspec

Self Cite

View full text Add to dashboard Cite

The red‐vine‐leaf extract AS195 improves cutaneous oxygen supply and the microcirculation in patients suffering from chronic venous insufficiency. Regulation of blood flow was associated to nitric oxide synthase (NOS)‐dependent NO (nitric oxide) production, and endothelial and red blood cells (RBC) have been shown to possess respective NOS isoforms. It was hypothesized that AS195 positively affects NOS activation in human umbilical vein endothelial cells (HUVECs) and RBC. Because patients with microvascular disorders show increased oxidative stress which limits NO bioavailability, it was further hypothesized that AS195 increases NO bioavailability by decreasing the content of reactive oxygen species (ROS) and increasing antioxidant capacity. Cultured HUVECs and RBCs from healthy volunteers were incubated with AS195 (100 μmol/L), tert‐butylhydroperoxide (TBHP, 1 mmol/L) to induce oxidative stress and with both AS195 and TBHP. Endothelial and red blood cell–nitric oxide synthase (RBC‐NOS) activation significantly increased after AS195 incubation. Nitrite concentration, a marker for NO production, increased in HUVEC but decreased in RBC after AS195 application possibly due to nitrite scavenging potential of flavonoids. S‐nitrosylation of RBC cytoskeletal spectrins and RBC deformability were increased after AS195 incubation. TBHP‐induced ROS were decreased by AS195, and antioxidative capacity was significantly increased in AS195‐treated cells. TBHP also reduced RBC deformability, but reduction was attenuated by parallel incubation with AS195. Adhesion of HUVEC was also reduced after AS195 treatment. Red‐vine‐leaf extract AS195 increases NOS activation and decreases oxidative stress. Both mechanisms increase NO bioavailability, improve cell function, and may thus account for enhanced microcirculation in both health and disease.

show abstract

“…Moreover, eNOS has been suggested to modulate the development of epithelial cells of the enteric mucosa (Korkmaz et al, 2007) and…”

Section: Vwf and Enos Immunoreactivitymentioning

confidence: 99%

Birthweight determines intestinal microvasculature development and alters endothelial nitric oxide synthase density in young piglets

Ayuso

Cruchten

Ginneken

2020

Anat Histol Embryol

View full text Add to dashboard Cite

Blood supply to enterocytes dictates intestinal health and nutrient absorption. These two aspects are impaired in low birthweight (LBW) piglets, but whether the perfusion to intestinal tissues is implicated as well is still unknown. Thus, structural changes in the microvasculature of LBW and normal birthweight (NBW) piglets were investigated during early postnatal development. Additionally, the presence of endothelial nitric oxide synthase (eNOS) in the intestinal mucosa was assessed given its important role to assure perfusion. A total of 22 pigs (11 LBW and 11 NBW) were sacrificed at days 0, 3, 8 and 19 of life. Body weight and intestinal length were recorded and a piece of the small intestine was sampled for immunohistochemical analysis of von Willebrand Factor (vWF, an endothelial cell marker) and eNOS. LBW piglets had a relatively (to body weight) longer intestine than their NBW counterparts. Age did not affect microvasculature, which was more abundant (85% larger vWF‐positive area) in NBW than LBW pigs. However, an interaction age*BW was observed for eNOS‐IR, showing that eNOS presence peaked in NBW piglets on the first day of life and subsequently decreased. This pattern was not observed in LBW piglets. The less abundant intestinal endothelial mass and the different pattern of eNOS expression observed in LBW piglets suggests microcirculation as a contributing factor in the impaired digestive functioning and gut health of LBW pigs. However, revealing whether the origin of this alteration is prenatal or postnatal, for example due to a lower milk intake, needs further study.

show abstract

The developmental stage and cell type dependent phosphorylation of eNOS in murine enteric mucosa and myenteric plexus

Cited by 4 publications

References 28 publications

Chronic alcohol consumption induces an overproduction of NO by nNOS- and iNOS-expressing myenteric neurons in the murine small intestine

Chronic alcohol consumption induces an overproduction of NO by nNOS- and iNOS-expressing myenteric neurons in the murine small intestine

The red‐vine‐leaf extract AS195 increases nitric oxide synthase–dependent nitric oxide generation and decreases oxidative stress in endothelial and red blood cells

Birthweight determines intestinal microvasculature development and alters endothelial nitric oxide synthase density in young piglets

Contact Info

Product

Resources

About