The developmental relationship between the deciduous dentition and the oral vestibule in human embryos

Hovořáková, Mária; Lesot, Hervé; Peterka, Miroslav; Peterková, Renata

doi:10.1007/s00429-004-0441-y

Cited by 33 publications

(83 citation statements)

References 17 publications

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“…We propose that the ectopic flange of epithelium might correspond to the vestibular lamina fused with MUT-M 1 as a consequence of failed differentiation of the oral vestibule in the lower jaw of MUT mice. In the upper jaw, fusion between vestibular and dental lamina occurs physiologically in mice (Peterkova et al, 2002b) and humans (Hovorakova et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

The Prx1 Homeobox Gene is Critical for Molar Tooth Morphogenesis

et al. 2006

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The paired-related homeobox genes, Prx1 and Prx2, encode transcription factors critical for orofacial development. Prx1 -/-/Prx2 -/-neonates have mandibular hypoplasia and malformed mandibular incisors. Although the mandibular incisor phenotype has been briefly described (ten Berge et al., 1998(ten Berge et al., , 2001Lu et al., 1999), very little is known about the role of Prx proteins during tooth morphogenesis. Since the posterior mandibular region was relatively normal, we examined molar tooth development in Prx1 -/-/Prx2 -/-embryos to determine whether the tooth malformation is primary to the loss of Prx protein or secondary to defects in surrounding tissues. Three-dimensional (3D) morphological reconstructions demonstrated that Prx1 -/-/Prx2 -/-embryos had molar malformations, including cuspal changes and ectopic epithelial projections. Although we demonstrate that Prx1 protein is expressed only mesenchymally, 3D reconstructions showed important morphological defects in epithelial tissues at the cap and bell stages. Analysis of these data suggests that the Prx homeoproteins are critical for mesenchymal-epithelial signaling during tooth morphogenesis.

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Section: Discussionmentioning

confidence: 99%

The Prx1 Homeobox Gene is Critical for Molar Tooth Morphogenesis

et al. 2006

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“…In the human embryo, this process begins during the 6th week of prenatal development [4] . At the bud stage, the epithelium of the dental lamina grows into the underlying neural-crest-derived mesenchyme and forms an epithelial bud, around which the mesenchymal cells condense [2,5] .…”

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confidence: 99%

Investigation of Proliferative Activity in the Developing Human Tooth Using Ki-67 Immunostaining

Guven

Günhan²,

Akbulut³

et al. 2007

Med Princ Pract

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Objective: The aim of this study was to investigate the proliferation of the developing human tooth germ and its surrounding tissues using Ki-67 immunostaining. Materials and Methods: Sections of mandibular dental arch tissues collected from 4 cadaveric human fetuses of 13, 16, 21 and 30 weeks of gestation were used. The immunoreactivity of Ki-67 in the tissue sections was assessed visually under a light microscope. Immunohistochemical controls were performed by replacing the primary antibody with phosphate-buffered saline or normal rabbit lgG. Results: The control sections did not display Ki-67 immunoactivity. Specimens of 13 weeks of gestation revealed intense Ki-67 immunostaining throughout the entire developing mandibular primary molars. At 16 weeks of gestation, immunostaining was observed in the inner enamel epithelium and dental papilla, in conjunction with the dental lamina showing decreased immunostaining. At 21 weeks, Ki-67 immunostaining was observed only in the inner enamel epithelium and dental papilla. The immunoreactivity of active ameloblasts and odontoblasts decreased, along with the proliferation capacity of the dental lamina. At 30 weeks, both enamel and dentin formation was observed along the cusped aspect of the tooth germ. Ameloblasts and odontoblasts were no longer immunoreactive in this region, while both types of cells were immunoreactive at the cervical regions of the crown. Dental lamina cells showed disintegration and were totally Ki-67-negative at 30 weeks of gestation. Conclusion: The Ki-67 immunoreactivity of the dental lamina decreased during intrauterine tooth development. Positive immunostaining was observed at specific sites in the enamel organ and dental papilla during the cap and bell stages.

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“…In the maxilla, the thickening of the oral epithelium starts during the sixth embryonic week. In the upper jaw quadrant, the dental lamina is constituted from two parts separated by a deep gap at the site of the earlier fusion of the medial nasal and maxillary facial outgrowth at 40 days (Ooe, '56;Hovorakova et al, 2005). At 44 days the dental lamina represents a continuous common area where it is possible to determine distinct epithelial swellings corresponding to the primordial germs of the deciduous incisors, canine and first molar.…”

Section: Discussion Of a Dental Lamina And The Human Dentitionmentioning

confidence: 99%

“…Additional epithelial thickenings are also created on each lateral border of the fronto-nasal processes. Thereafter, these epithelial thickenings fuse and form a continuous plate of epithelium for both upper and lower jaws in humans (Ooe, '56;Nery et al, '70;Hovorakova et al, 2005Hovorakova et al, , 2007. All teeth will arise from this epithelium (the odontogenic band defined from molecular data) as it later forms a continuous dental lamina.…”

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confidence: 96%

Dental lamina as source of odontogenic stem cells: evolutionary origins and developmental control of tooth generation in gnathostomes

2009

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This study considers stem cells for odontogenic capability in biological tooth renewal in the broad context of gnathostome dentitions and the derivation of them from oral epithelium. The location of the developmental site and cell dynamics of the dental lamina are parameters of a possible source for odontogenic epithelial stem cells, but the phylogenetic history is not known. Understanding the phylogenetic basis for stem cell origins throughout continuous tooth renewal in basal jawed vertebrates is the ultimate objective of this study. The key to understanding the origin and location of stem cells in the development of the dentition is sequestration of stem cells locally for programmed tooth renewal. We suggest not only the initial pattern differences in each dentate field but local control subsequently for tooth renewal within each family. The role of the specialized odontogenic epithelium (odontogenic band) is considered as that in which the stem cells reside and become partitioned. These regulate time, position and shape in sequential tooth production. New histological data for chondrichthyan fish show first a thickening of the oral epithelium (odontogenic band). After this, all primary and successive teeth are only generated deep to the oral epithelium from a dental lamina. In contrast, in osteichthyan fish the first teeth develop directly within the odontogenic band. In addition, successors are initiated at each tooth site in the predecessor tooth germ (without a dental lamina). We suggest that stem cells specified for each tooth family are set up and located in intermediate cells between the outer and inner dental epithelia.

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The developmental relationship between the deciduous dentition and the oral vestibule in human embryos

Cited by 33 publications

References 17 publications

The Prx1 Homeobox Gene is Critical for Molar Tooth Morphogenesis

The Prx1 Homeobox Gene is Critical for Molar Tooth Morphogenesis

Investigation of Proliferative Activity in the Developing Human Tooth Using Ki-67 Immunostaining

Dental lamina as source of odontogenic stem cells: evolutionary origins and developmental control of tooth generation in gnathostomes

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