The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: association with vascular endothelial growth factor levels and perifollicular blood flow characteristics

Blerkom, Jonathan Van; Antczak, M; Schrader, Roland Matthias

doi:10.1093/humrep/12.5.1047

Cited by 489 publications

(313 citation statements)

References 14 publications

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“…56 High frequencies of chromosome abnormalities because of segregation disorders in oocytes from severe hypoxic follicles have also been found. 57 It is not only age-dependent factors that play a role in the production of aneuploid oocytes; an individual predisposition originating during early meiotic stages could also be very important. Synaptic errors between homologous chromosomes, 58 a reduction in the number of chiasmata because of a low recombination rate 59 and the subsequent segregation of these unstable bivalents, 60 an altered DNA repair because of defects in sister chromatid cohesion 61 and other mechanisms are being found to play an important role.…”

Section: Aneuploidy Ratementioning

confidence: 99%

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

et al. 2003

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We used fluorescent in situ hybridisation (FISH) to detect nine chromosomes (1,13,15,16,17,18,21,22 and X) in 89 first Polar Bodies (1PBs), from in vitro matured oocytes discarded from IVF cycles. In 54 1PBs, we also analysed the corresponding oocyte in metaphase II (MII) to confirm the results; the other 35 1PBs were analysed alone as when preimplantation genetic diagnosis using 1PB (PGD-1PB) is performed. The frequency of aneuploid oocytes found was 47.5%; if the risk of aneuploidy for 23 chromosomes is estimated, the percentage rises to 57.2%. Missing chromosomes or chromatids found in 1PBs of 1PB/MII doublets were confirmed by MII results in 74.2%, indicating that only 25.8% of them were artefactual. Abnormalities observed in 1PBs were 55.8% whole-chromosome alterations and 44.2% chromatid anomalies. We observed a balanced predivision of chromatids for all chromosomes analysed. Differences between balanced predivision in 1PB and MII were statistically significant (Po0.0001, v 2 test); the 1PB was most affected. The mean abnormal segregation frequency for each chromosome was 0.89% (range 0.52-1.70%); so, each of the 23 chromosomes of an oocyte has a risk of 0.89% to be involved in aneuploidy. No significant differences were observed regarding age, type of abnormality (chromosome or chromatid alterations) or frequency of aneuploidy. Nine of the 35 patients (25.7%) whose 1PB and MII were studied presented abnormalities (extra chromosomes) that probably originated in early oogenesis. Analysis of 1PBs to select euploid oocytes could help patients of advanced age undergoing in vitro fertilization (IVF) treatment.

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Section: Aneuploidy Ratementioning

confidence: 99%

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

et al. 2003

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“…Multinucleation has also been associated with higher blastomere diameter [1,6], decrease in blastocyst formation rate [5,7,15,19], reduced capacity of development on siblings of MN embryos [2,5], high amount of oocytes retrieved and elevated E 2 levels [2,3,20], follicles hypoxia [21], culture media [13,22], the use of GnRH antagonist [23] and apoptosis [6].…”

Section: Introductionmentioning

confidence: 99%

Insights on blastomere nuclearity

Gil

Gustavo

Póo

et al. 2006

J Assist Reprod Genet

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Purpose: To analyze the results of our transferred embryos, especially those that "changed" their blastomere nuclearity from Multinucleated (MN) to Mono-nucleated during development.Methods: Pregnancies where at least one MN embryo was transferred were retrospectively evaluated and categorized in order to record and follow-up on the ones that were implanted. Embryos were classified as normal (when all blastomeres were mono-nucleated on day one and two of development), corrected (multinucleated embryos on day one that became mono-nucleated on day two) and non-corrected (multinucleated either on day one, on day two or both days).Results: There were 633 transfer cycles analyzed. Thirtythree percent (206) had at least one embryo with a MN blastomere at a given stage of development. Pregnancy and implantation rates were 29.0% and 19.0% for the group of exclusively mono-nucleated embryo transfers, and 28.6% and 15.8% for the group with at least one MN embryo transferred. The pregnancy outcome for "corrected" and "noncorrected" embryos could be corroborated unequivocally in only 9 cases, with an outcome of 8 and 4 normal babies, respectively.Conclusions: Because the amount of data analyzed is not satisfactorily large, differences were not significantly different; however, a trend may exist showing that normal at term pregnancies obtained from corrected embryos are more likely to occur than those from non-corrected embryos. Nuclear observation on a daily basis should be one of the strate-Clínica de Fertilidad, Centro Médico Docente La Trinidad, Caracas, Venezuela e-mail: mgilco@cantv.net gies used to select the best embryos for transferring, to improve implantation rates and avoid multiple pregnancies.

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“…[9][10][11][12][13] Otherwise, follicle blood flow as a parameter of the ovarian competence is carried out in the stimulated ovary throughout the human chorionic gonadotropin administration day. 14,15 The aim of this study was to evaluate the intraobserver and interobserver reproducibility of the ovarian volume, antral follicle count, and ovarian 3D vascularity indices, calculated from the rotational method with the use of the VOCAL imaging program, depending on the ovarian functional stage. Two groups of patients were considered: basal group, evaluated after gonadotropin-releasing hormone (GnRH) agonist pituitary suppression; and stimulated group, after controlled ovarian stimulation.…”

mentioning

confidence: 99%

Intraobserver and Interobserver Reproducibility of Ovarian Volume, Antral Follicle Count, and Vascularity Indices Obtained With Transvaginal 3-Dimensional Ultrasonography, Power Doppler Angiography, and the Virtual Organ Computer-Aided Analysis Imaging Pr

Mercé

Gómez

Engels

et al. 2005

Journal of Ultrasound in Medicine

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Objectives. The purpose of this study was to assess intraobserver and interobserver reproducibility of the parameters of ovarian response and oocyte ability, studied by 3-dimensional ultrasonography and power Doppler angiography (PDA), and the possible influence of the ovarian functional stage. Methods. Twenty-nine women were included in an in vitro fertilization program. Fourteen women were evaluated after pituitary suppression (basal group), and 15 were scanned on the human chorionic gonadotropin administration day, after gonadotropin ovarian stimulation (stimulated group). A first observer acquired 2 volumes for each ovary. Another observer performed a second analysis of the volumes acquired by the first observer. We analyzed ovarian volume, follicle number in the basal group, vascularization index, flow index, and vascularization-flow index. The volumes were processed by the Virtual Organ Computer-Aided Analysis imaging program using plane A and 15°rotational steps. Results. Ovarian volume showed excellent intraobserver and interobserver agreement, with an intraclass correlation coefficient (Intra-CC) and an interclass correlation coefficient (Inter-CC) close to the unit. The Intra-CC and Inter-CC about the number of follicles were 0.964 and 0.978, respectively. Vascularity indices showed an Intra-CC greater than 0.90. The vascularization index and the vascularization-flow index showed higher interobserver than intraobserver reproducibility (Inter-CC > 0.96 for both). The flow index Inter-CC was 0.898. The reproducibility differences between the basal and stimulated ovary measurements were not significant. Conclusions. There is an excellent intraobserver and interobserver reproducibility of the ovarian volume, follicle counts, and 3-dimensional PDA indices. The ovarian functional stage has no influence on the reliability. Three-dimensional ultrasonography and PDA improve the study of ovarian parameters, and their reliability impels a change in the current clinical routine of performing and interpreting ultrasonography.

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The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid: association with vascular endothelial growth factor levels and perifollicular blood flow characteristics

Cited by 489 publications

References 14 publications

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

Analysis of nine chromosome probes in first polar bodies and metaphase II oocytes for the detection of aneuploidies

Insights on blastomere nuclearity

Intraobserver and Interobserver Reproducibility of Ovarian Volume, Antral Follicle Count, and Vascularity Indices Obtained With Transvaginal 3-Dimensional Ultrasonography, Power Doppler Angiography, and the Virtual Organ Computer-Aided Analysis Imaging Pr

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