The developmental fate of S. coelicolor hyphae depends upon a gene product homologous with the motility σ factor of B. subtilis

Chater, Keith F.; Bruton, Celia J.; Plaskitt, Kitty A.; Buttner, Mark J.; Méndez, Cármen; Helmann, John D.

doi:10.1016/0092-8674(89)90876-3

Cited by 174 publications

(148 citation statements)

References 48 publications

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“…Interestingly, S. coelicolor GSA2, which is optimized for the stable overexpression of ssgA, produces very small mycelial fragments and even chains of immature spores. Stimulation of sporulation was also observed when the developmental sigma factor WhiG was overproduced in S. coelicolor, although the level and frequency of spore formation were lower in that case (11). This suggests that the machinery required for submerged sporulation is present in S. coelicolor.…”

Section: Discussionmentioning

confidence: 73%

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

et al. 2000

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The role of ssgA in cell division and development of streptomycetes was analyzed. An ssgA null mutant of Streptomyces coelicolor produced aerial hyphae but failed to sporulate, and ssgA can therefore be regarded as a novel whi gene. In addition to the morphological changes, antibiotic production was also disturbed, with strongly reduced actinorhodin production. These defects could be complemented by plasmid-borne ssgA. In the wild-type strain, transcription of ssgA was induced by nutritional shift-down and was shown to be linked to that of the upstream-located gene ssgR, which belongs to the family of iclR-type transcriptional regulator genes. Analysis of mycelium harvested from liquid-grown cultures by transmission electron microscopy showed that septum formation had strongly increased in ssgA-overexpressing strains in comparison to wild-type S. coelicolor and that spore-like compartments were produced at high frequency. Furthermore, the hyphae were significantly wider and contained irregular and often extremely thick septa. These data underline the important role for ssgA in Streptomyces cell division.

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Section: Discussionmentioning

confidence: 73%

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

et al. 2000

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“…3). (32) and WhiG (10). Identical residues in at least two of the three protein sequences are indicated in boldface type.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the sigD transcription unit of Bacillus subtilis

Márquez-Magaña

Chamberlin

1994

J Bacteriol

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The 0FD factor of BaciUlus subtilis is required for the transcription of the flagellin and motility genes as well as for wild-type chemotaxis. Southern blot and sequence analyses demonstrate that the structural gene for SFD, sigD, is located immediately downstream of a region of DNA originally identified as the chemotaxis (che) locus and now renamed thefla/che region. In fact, sigD appears to be part of a very large operon (>26 kb) containing genes which encode structural proteins that form the hook-basal body complex as well as regulatory proteins required for chemotaxis. Transposon insertions up to 24 kb upstream of sigD, within several of the genes for the hook-basal body components, give rise to only a moderate decrease in sigD expression. The transposon insertions, however, block CrD activity as demonstrated by the lack of flagellin expression in strains bearing these insertions. These effects appear to arise from two types of regulation. In cis the transposon insertions appear to introduce a partial block to transcription of sigD from upstream promoter elements; in trans they disrupt genes whose gene products are required for oD activity. It appears that sigD transcription is initiated, at least in part, by a promoter many kilobases upstream of its translation start site and that transcription of the flagellin gene by SFD is dependent on the formation of a functional hook-basal body complex. The possibility that sigD is part of the Ia/che operon was further tested by the integration of an insertion plasmid, containing strong transcription terminators, 1.6 and 24 kb upstream of the sigD gene. In both cases, the introduction of the terminators resulted in a greater decrease of sigD expression than was caused by the plasmid sequences alone. These results indicate that wild-type transcription of sigD is dependent on promoter sequences >24 kb upstream of its structural gene and that the entirefla/che region forms a single operon.

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“…Analysis of Streptornyces promoters has revealed a high degree of sequence heterogeneity (Strohl, 1992;Bourn & Babb, 1995), much of which may be attributable to the occurrence of multiple Q factors (Westpheling et al, 1985;Chater et al, 1989;Buttner et al, 1990;Tanaka et al, 1991;Lonetto et al, 1994;Potuckovi et al, 1995). Interestingly, Streptomyces coelicolor A3 (2), the most genetically characterized streptomycete, possesses a family of four very similar Q factors (Tanaka et al, 1991) that includes the major and essential Q factor, HrdB (Buttner et al, 1990;Brown et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage I19 using the gentamicin resistance gene (aacC1) of Tn1696 as reporter

1997

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A promoter-probe shuttle plasmid (pGL7011) containing the promoterless aminoglycoside-O-acetyltransferase I gene (aacC7) of Tn1696 was used to isolate DNA fragments from Streptomyces ghanaensis phage 119 that possessed promoter activity in Streptomyces liwidans TK23. Analysis of gentamicin (Gm) resistance levels in Escherichia coli and in 5. liwidans TK23, and of aacC7 mRNA levels in 5. liwidans, identified a fragment (F14) that exhibited a high level of promoter activity in both species. Subsequent analysis revealed that the promoter activity of SF14 (a subcloned fragment of F14) was about twice that of erm€p*, one of the strongest characterized actinomycete promoters. SF14 contained two tandemly arranged promoters, 14-lp and p14-11p, with overlapping and adjacent -10 and -35 regions, respectively. Both promoters appear to be recognized with different efficiencies by the major RNA polymerase holoenzyme (Edd? of Streptomyces coelicolor A3(2).

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The developmental fate of S. coelicolor hyphae depends upon a gene product homologous with the motility σ factor of B. subtilis

Cited by 174 publications

References 48 publications

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

ssgA Is Essential for Sporulation of Streptomyces coelicolor A3(2) and Affects Hyphal Development by Stimulating Septum Formation

Characterization of the sigD transcription unit of Bacillus subtilis

Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage I19 using the gentamicin resistance gene (aacC1) of Tn1696 as reporter

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