The Development of Stress-resistant Stock of Spring Bread Wheat by in Vitro Cell Selection

Nikitina, E. I.; Khlebova, L. P.; Соколова, Г. Г.; Ereschenko, Olga

doi:10.14258/izvasu(2013)3.2-20

Cited by 5 publications

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“…However, Hess & Carman (1998), Pastori et al (2001) stated the regeneration efficiency of the explant affected high by its physiological status, depending mainly on environmental temperature before collecting samples. According to our studies made earlier, we have observed that the reactions of some genotypes of wheat varied depending on the maternal environment (Grigorieva & Shletser, 2006;Nikitina et al, 2013b;. In addition, Hess & Carman (1998) reported that individual explants of incompetent lines sometimes occasionally revealed a favorable response to in vitro culture suggesting a more critical assessment of donor growth conditions.…”

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Morphogenetic Responses of Wheat Immature Embryo Culture Depending on Growing Conditions of Donor Plants

Khlebova

Nikitina

2016

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The article considers the influence of growth conditions of donor plants on in vitro morphogenetic responses of immature embryos from spring bread wheat. The materials were 12 varieties of different ecological and geographical origin from the collection of Altai Research Institute of Agriculture (Barnaul, Russia). Donor plants were grown in the greenhouse and experimental field near Barnaul. Immature embryos with 1.3-1.5 mm in size isolated from seeds collected 14-16 days post anthesis were used as explants. The embryos were placed with the sculletum upwards on a sterile solid agar (0,8%) medium containing the inorganic components of Linsmaier & Skoog (LS), 3% sucrose, 2.0 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) and incubated in the darkness at 26±1°C. Induced calli were subcultured after 25-30 days interval in fresh medium supplemented with 0.5 mg l -1 2,4-D and 0.5 mg l -1 kinetin and grown at 25±1 °C under a 16/8-h (day/night) photoperiod with a white fluorescent light. Embryogenic calli were transferred to LS medium containing 0.2 mg l -1 indole-3-acetic acid (IAA) for regeneration. A complete randomized block design with four replications per a genotype (60 embryos) was used. We stated by analysis of the components of phenotypic variance that genetic factor has been the leading one in all stages of tissue culture, but the highest effect (80%) was on plant regeneration. Interaction of the factors considered was competitive with genotypic influence on the stages of morphogenesis (28%) and callusogenesis (27%). Influence of "conditions" was statistically significant, but inferior to other factors. The greatest result was obtained for induction of morphogenic calli. Field conditions were the most favorable for genotypes with high callus and regeneration abilities. Culture responses of those with a low regeneration potential increased after growing donor plants in greenhouse vegetation in 3.4 -13.9 times. For most genotypes moderate temperature and precipitation as well as natural lighting before collecting material increased morphogenic competence of explants in wheat tissue culture. Users are permitted to copy, use, distribute, transmit, and display the work publicly and to make and distribute derivative works, in any digital medium for any responsible purpose, subject to proper attribution of authorship. содержащей 0,8% агара, 3% сахарозы, 2,0 мг/л дихлорфеноксиуксусной кислоты (2,4-Д), в темноте при температуре 26±1 °C. Через 25-30 дней каллусы переносили на дифференцирующую среду LS, дополненную 0,5 мг/л 2,4-Д и 0,5 мг/л кинетина и выращивали на свету с 16-часовом фотопериодом (день) и температуре 25±1°C. Морфогенные каллусы переносили на среду для регенерации, содержащую 0,2 мг/л индолил-3-уксусной кислоты (ИУК). Эксперимент выполнен в четырех повторениях по 60 эксплантов на генотип. Установлено, что генетический фактор является ведущим на всех стадиях культуры ткани с максимальным эффектом на этапе регенерации (80%). Доля фактора «взаимодействие генотип-условия» была примерно одинакова с генотипич...

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Morphogenetic Responses of Wheat Immature Embryo Culture Depending on Growing Conditions of Donor Plants

Khlebova

Nikitina

2016

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“…В таблицах представлены средние арифметические значения и ошибки средних. Установлено, что при достаточно длительном закаливании эксплантов в течение 12 и 16-ти суток при всех режимах промораживания (варианты опыта: 3,4, 7,8,11,12,15,16,19,20) зародыши темнели и не прорастали, поэтому эти варианты не приведены в таблицах и на рисунках (для сокращения их объема). Полученные результаты свидетельствуют о целесообразности использования при селекции in vitro меньших экспозиций закаливания (от 4 до 8 суток).…”

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Use of Embrio Culture for Selection in Vitro of Coriander Forms, Resistant to Low-Temperature Stress

Yegorova¹,

Ставцева²

2019

ÈKOBIOTEH

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In Vitro Culture of Autonomous Embryos as a Model System for the Study of Plant Stress Tolerance to Abiotic Factors (on the Example of Cereals)

Круглова

Zinatullina

2022

Biol Bull Rev

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The Development of Stress-resistant Stock of Spring Bread Wheat by in Vitro Cell Selection

Cited by 5 publications

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Morphogenetic Responses of Wheat Immature Embryo Culture Depending on Growing Conditions of Donor Plants

Morphogenetic Responses of Wheat Immature Embryo Culture Depending on Growing Conditions of Donor Plants

Use of Embrio Culture for Selection in Vitro of Coriander Forms, Resistant to Low-Temperature Stress

In Vitro Culture of Autonomous Embryos as a Model System for the Study of Plant Stress Tolerance to Abiotic Factors (on the Example of Cereals)

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