The development of species-specific SCAR markers for delimitation of<i>Calypogeia</i>species

Buczkowska, Katarzyna; Dabert, Mirosława

doi:10.1179/1743282011y.0000000029

Cited by 12 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The atypical form represents a new taxon tentatively named as C. sp. nov. (Buczkowska & Dabert 2011). The new taxon morphologically resembles C. muelle riana s.…”

Section: Introductionmentioning

confidence: 81%

“…Two sequences from GenBank determined as C. muelleriana were compared with the sequences of the C. muelleriana complex obtained in this study. The sequence of the trnL gene intron from United Kingdom (Ay453769) is the same as in C. Explanation: 1 -the new species detected within the C. muelleriana complex (Buczkowska & Dabert (2011) muelleriana s. str., whereas the sequences of introns of trnL and trnG genes from USA (Ay608121 and Ay608169) belong to the new taxon -C. sp.…”

Section: Resultsmentioning

confidence: 99%

“…1 -sequences new in the present study are marked in bold, 2 -the new species detected within the C. muelleriana complex (Buczkowska & Dabert (2011 Primers used for the amplification and sequencing of trnHpsbA spacer (psbAF and trnHR) were according to Sang et al (1997) and for the introns of trnG and trnL genes (primers A and B) from Pacak & Szweykowska- Kulińska (2003). PCR amplification was carried out as described in the previous publication (Buczkowska et al 2012a).…”

Section: Explanationsmentioning

confidence: 99%

“…nov. (Table 1). To obtain plant material free from contamination for DNA extraction, in vitro cultures of the studied species were established according to Buczkowska et al (2006) and Buczkowska & Dabert (2011). In the second stage of research, DNA was extracted from herbarium samples.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The typical form is widespread in south-western part of Poland, whereas the atypical form occurs in the northern part of lowlands. Isozyme and molecular markers have proved that these two forms are genetically different (Buczkowska & Bączkiewicz 2011;Buczkowska & Dabert 2011). Plants classified as the typical form exactly correspond to the type specimen of C. muelleriana (Buczkowska 2004b).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Chloroplast Dna Sequences Confirmed Genetic Divergence Within Calypogeia Muelleriana (Calypogeiaceae, Marchantiophyta)

Buczkowska

Gonera

Bączkiewicz

et al. 2013

Biodiversity Research and Conservation

Self Cite

View full text Add to dashboard Cite

Nine species of the genus Calypogeia Raddi are currently known from Europe: C. azurea, C. integristipula, C. neesiana, C. suecica, C. muelleriana, C. sphagnicola, C. fissa, C. arguta, and C. azorica. Recently, another species, morphologically resembling C. muelleriana but genetically distinct from it, was detected using isozyme markers. In the present study, relationships between the newly detected species (C. sp. nov.) and typical C. muelleriana were analyzed using the DNA sequences data of three regions from the chloroplast genome: introns of trnG and trnL genes and intergenic spacer trnH-psbA. Calypogeia sp. nov. differs from C. muelleriana s. str. (typical form) in all examined chloroplast regions. It differs as well from C. azurea, which was used as a reference species. The number of fixed nucleotide differences between C. muelleriana s. str. and C. sp. nov. is almost the same as between C. muelleriana s. str. and C. azurea. The results of the present study suggest a closer affinity of C. sp. nov. to C. azurea than to C. muelleriana s. str. in Europe, C. muelleriana s. str. was noted in Poland, Germany, Holland, United Kingdom and Azores. Samples determined as C. sp. nov., besides Poland, were so far detected also in North America.

show abstract

“…The atypical form represents a new taxon tentatively named as C. sp. nov. (Buczkowska & Dabert 2011). The new taxon morphologically resembles C. muelle riana s.…”

Section: Introductionmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Section: Explanationsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chloroplast Dna Sequences Confirmed Genetic Divergence Within Calypogeia Muelleriana (Calypogeiaceae, Marchantiophyta)

Buczkowska

Gonera

Bączkiewicz

et al. 2013

Biodiversity Research and Conservation

Self Cite

View full text Add to dashboard Cite

show abstract

Allopolyploid speciation of Calypogeia sphagnicola (Jungermanniopsida, Calypogeiaceae) based on isozyme and DNA markers

et al. 2011

Self Cite

View full text Add to dashboard Cite

Calypogeia sphagnicola is one of nine species of the genus Calypogeia known in Europe. Occurrence of the species is closely connected with peat bogs. Nowadays, two forms of this species are distinguished-C. sphagnicola f. sphagnicola and C. sphagnicola f. paludosa. The results of the present study, based on two classes of markers-isozymes and sequences of chloroplast genom (trnH-psbA, rpoC1)-unanimously support the genetic differentiation within the taxon and show that the presentday forms represent genetically distinct species. Phylogenetic analysis resolved two lineages that correspond with the present-day forms with high bootstrap support, which differ in ploidy level: C. sphagnicola f. sphagnicola is haploid, whereas C. sphagnicola f. paludosa is a diploid form. Allopolyploid origin of the diploid form was revealed by the isozyme pattern. Nei's genetic distance between the two present-day forms of C. sphagnicola was 0.472. The forms in Poland have an allopatric pattern of geographic distribution: C. sphagnicola f. sphagnicola occurs exclusively in the lowlands of the northern part of the country on raised peat bogs, whereas C. sphagnicola f. paludosa is found only in the mountains of southern Poland, mainly in the subalpine zone, where it grows on Sphagnum-Polytrichum hummocks on the upper part of north-facing slopes. Plants regarded in this study as C. sphagnicola f. sphagnicola morphologically correspond to the syntype specimen of C. sphagnicola.

show abstract

Effectiveness of ISSR markers for determination of the Aneura pinguis cryptic species and Aneura maxima

Buczkowska

Rabska

Gonera

et al. 2016

Biochemical Systematics and Ecology

View full text Add to dashboard Cite

The development of species-specific SCAR markers for delimitation ofCalypogeiaspecies

Cited by 12 publications

References 32 publications

Chloroplast Dna Sequences Confirmed Genetic Divergence Within Calypogeia Muelleriana (Calypogeiaceae, Marchantiophyta)

Chloroplast Dna Sequences Confirmed Genetic Divergence Within Calypogeia Muelleriana (Calypogeiaceae, Marchantiophyta)

Allopolyploid speciation of Calypogeia sphagnicola (Jungermanniopsida, Calypogeiaceae) based on isozyme and DNA markers

Effectiveness of ISSR markers for determination of the Aneura pinguis cryptic species and Aneura maxima

Contact Info

Product

Resources

About