2016
DOI: 10.1007/s12088-016-0608-2
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The Development of Simple Methods for the Maintenance and Quantification of Polymyxa graminis

Abstract: , a root endoparasite of several cereal species, is considered to be non-pathogenic but serves as a vector of various plant viruses belonging to the genera ,, and . Specifically, it reduces barley productivity by transmitting the (BaYMV). To date, due to its obligate biotrophic property, no artificial culturing of was reported and its quantification was also technically challenging. Here, we developed a novel and simple method to infect within sterile barley roots in contamination free by preparing nearly pure… Show more

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Cited by 4 publications
(4 citation statements)
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“…The morphological structures found correspond to those described for P. graminis by Ledingham () and are similar to those observed by Tyagi et al . (), who analysed the roots of barley and observed the different phases of the P. graminis life cycle. The primer pair Pgfwd2/Pxrev7 is specific for P. graminis and amplification using this primer pair resulted in amplified fragments of the expected sizes 310 bp and 240 bp (Ward & Adams, ).…”
Section: Discussionmentioning
confidence: 99%
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“…The morphological structures found correspond to those described for P. graminis by Ledingham () and are similar to those observed by Tyagi et al . (), who analysed the roots of barley and observed the different phases of the P. graminis life cycle. The primer pair Pgfwd2/Pxrev7 is specific for P. graminis and amplification using this primer pair resulted in amplified fragments of the expected sizes 310 bp and 240 bp (Ward & Adams, ).…”
Section: Discussionmentioning
confidence: 99%
“…PCR conditions were in accordance with the work of Tyagi et al . (). The amplified fragments were subjected to electrophoresis in 1% agarose gel, stained with GelRed (Biotium), visualized under UV light, and photographed.…”
Section: Methodsmentioning
confidence: 97%
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“…The PCR mixture consisted of 5 μl of extracted total DNA (~1 μg), 2.5 μl of 10 × Taq reaction buffer, 0.75 μl of MgCl2 (25 mM), 0.5 μl of dNTPs (10 mM), 0.5 μl of each primer, and 1 U Platinum Taq DNA polymerase (Invitrogen). PCR conditions were in accordance with the work of Tyagi et al (2016). A sample of wheat root infected with P. graminis and a sample of P. graminis-free root were used as positive and negative controls for PCR, respectively.…”
Section: Characterization Of the Whsmv Host Rangementioning
confidence: 99%