The Development of Sheep Eggs in the Rabbit Oviduct and Their Viability After Re-Transfer to Ewes

Lawson, R. A. S.; Adams, C. E.; Rowson, L. E. A.

doi:10.1530/jrf.0.0290105

Cited by 58 publications

(11 citation statements)

References 16 publications

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“…The oviductal environment can support embryonic growth up to the blastocyst stage across a wide range of species following transspecies transfer. Ligated rabbit oviducts have been used extensively for the development of embryos from many species including sheep [6,43], cattle [9,20,74], and recovery at Day 14, demonstrating that bovine embryos can undergo continued development in the reproductive tract of ewes when transferred either as 4-cell embryos or as expanded or hatched blastocysts.…”

Section: Embryo Culture In Vivo Vs In Vitromentioning

confidence: 99%

Factors influencing oocyte and embryo quality in cattle

et al. 2001

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Section: Embryo Culture In Vivo Vs In Vitromentioning

confidence: 99%

Factors influencing oocyte and embryo quality in cattle

et al. 2001

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“…Moreover, the containers used are costly and should be returned with the concomitant expense. Some approaches have explored the feasibility of alternative methods using, for instance, intermediate recipients [2-4], or specific temperatures [5,6]. In these studies, 2-cell embryos were used, even though embryos of certain mice strains are prone to 2-cell blockage when cultured in suboptimal conditions [7].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivodevelopment of mice morulae after storage in non-frozen conditions

Hourcade

Pérez-Crespo

Serrano

et al. 2012

Reprod Biol Endocrinol

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BackgroundInterchange of genetically modified (GM) mice between laboratories using embryos provides several advantages. Not only is transport stress avoided, but also the health status of the recipient colony is not compromised. Embryos do not need to be shipped in frozen stage, which requires expensive packaging in addition to a certain degree of expertise in order to freeze and thaw them correctly. The aim of this study was to examine different storage conditions and their effect on embryo viability in order to establish the feasibility of practical, non-frozen conditions for embryo shipment.MethodsMouse morulae developed in vivo (collected from donors 2.5d post coitum) or in vitro (zygotes cultured until morulae stage) were stored, combining two different media (KSOMeq or KSOM-H) and temperatures (4 degrees C, 15 degrees C and 37 degrees C) throughout 24 or 48 hours. After storage in vitro viability was assessed determining percentage of development to blastocyst and total cell number. In vivo viability was determined based on the number of implantations and living fetuses after embryo transfer of stored embryos. The storage effect at the molecular level was assessed by studying a gene pool involved in early development by quantitative RT-PCR.ResultsIn vivo-produced morulae stored for 24 hours did not show differences in development up to the blastocyst stage, regardless of the storage type. Even though a decrease in the total cell number in vivo was observed, embryo development after embryo transfer was not affected. All 24 hour storage conditions tested provided a similar number of implantations and fetuses at day 14 of pregnancy. Morulae obtained from in vitro embryo culture collected at the 1-cell stage showed a decreased ability to develop to blastocyst after 24 hours of storage at 15degrees C both in KSOMeq and KSOM-H. Concomitantly, a significant decrease of embryo implantation rates after transfer to recipients was also found. In order to further characterize the effect of non-frozen storage combining a molecular approach with the ordinary in vitro culture evaluation, embryos collected at the morula stage were submitted to the same storage conditions described throughout 48 hours. In vitro culture of those embryos showed a significant decrease in their developmental rate to blastocyst in both KSOMeq and KSOM-H at 15degrees C, which also affected the total number of cells. Gene transcription studies confirmed significant alterations in retrotransposons (Erv4 and Iap) after 48 h of storage at 15degrees C.ConclusionsOur results show that both KSOMeq and KSOM-H can be equally used, and that several temperature conditions allow good survival rates in vitro and in vivo. Some of these storage conditions can substitute freezing in order to maintain embryo viability for 24–48 hours, providing a reliable and less demanding technical alternative for embryo interchanges.

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“…Attempts to have particles of various origins (Greenwald 1958; Hodgson et al. 1976; Denker 1982; Bourdage and Halbert 1984) and ova and embryos derived from other species (Lawson et al. 1972; Sirard et al.…”

Section: Introductionmentioning

confidence: 99%

“…This might contribute to failure in the establishment of pregnancy when transferring in vitro-cultured rabbit embryos (Seidel et al 1976;Binkerd and Anderson 1979). Attempts to have particles of various origins (Greenwald 1958;Hodgson et al 1976;Denker 1982;Bourdage and Halbert 1984) and ova and embryos derived from other species (Lawson et al 1972;Sirard et al 1985;Kasai et al 1993) encased in mucin by introducing them into a rabbit oviduct, have been successful in most, but not all cases (Yoshinaga and Adams 1967). Kasai et al (1993) found that the mucin coat may help rabbit and, under certain conditions also mouse embryos, to resist damage during refrigeration.…”

Section: Introductionmentioning

confidence: 99%

Coating of Objects Introduced into the Oviduct of Pseudopregnant Rabbit Does

El-Gayar

Holtz

2009

Reprod Domestic Animals

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This investigation addresses the possibility of providing mouse embryos or other foreign objects with a protective mucin coat by transferring them into the oviduct of a live rabbit doe. Mouse embryos at the 8 or 16-cell stage, rabbit oocytes and latex spheres resembling mouse embryos in size were transferred to the ligated oviducts of ovulation-induced rabbit does. The does were killed 24 h later to have their oviducts flushed. A large proportion of the latex spheres (89%) and of the ovulated oocytes of the recipient does (92%) was recovered. The recovery rates for transferred rabbit oocytes, either intact or with the zona pellucida removed, were 61% and 51%, respectively, whereas that for mouse embryos was extremely poor (20%). Rabbit oocytes with or without zona were enveloped in a thick mucin coat regardless whether they had been transferred or ovulated by the recipients. The same applied to empty rabbit zonae. Mouse embryos and latex spheres were also covered by a mucin coat, but it was four times thinner. While residing in the rabbit oviduct, the mouse embryos continued developing to a stage comparable to what would have been expected in situ. During the subsequent in vitro culture, mouse embryos continued developing to the expanded blastocyst stage. They did, yet, not hatch from the zona. It may be concluded that particles of various origins, when placed into the oviduct of ovulated rabbit does, will be provided with a mucin covering which is, however, considerably thinner than that surrounding oocytes or zonae pellucidae originating from rabbits.

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The Development of Sheep Eggs in the Rabbit Oviduct and Their Viability After Re-Transfer to Ewes

Cited by 58 publications

References 16 publications

Factors influencing oocyte and embryo quality in cattle

Factors influencing oocyte and embryo quality in cattle

In vitro and in vivodevelopment of mice morulae after storage in non-frozen conditions

Coating of Objects Introduced into the Oviduct of Pseudopregnant Rabbit Does

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