The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars

Li, C. D.; Rossnagel, B. G.; Scoles, G. J.

doi:10.1007/s001220051605

Cited by 144 publications

(161 citation statements)

References 34 publications

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“…crassa hybrids (Gradzielewska et al, 2010) and comfortably close to the highest PIC value that Powell et al (1996) observed using four screening methods in soybean (AFLP, RAPD, RFLP, and SSR; SSRs had a PIC of 0.60). On the other hand, Sarla et al (2005) with ISSRs and Li et al (2000) with SSRs determined PIC values similar to ours: 0.50 for a group of rice cultivars and 0.57 and 0.51 for Avena species and cultivars, respectively. Among winter triticale cultivars and breeding lines, the average PIC calculated on the basis of polymorphic SSR markers was also similar to the one we obtained in our study (0.54) (Tams et al, 2004).…”

Section: Discussionsupporting

confidence: 87%

Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs

Grądzielewska

Gruszecka²,

Leśniowska-Nowak³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Wide hybridization expands the gene pool of cultivated species and can produce genotypes with practical value, often resistant to diseases. In plants, ISSRs are often used for hybrid identification as well as for diversity evaluation. We estimated genetic similarity among advanced genetic stocks of triticale hybrids and identified Aegilops juvenalis introgressions into triticale. Fourteen ISSR primers amplified from 12 to 23 DNA fragments. The number of polymorphic products per primer ranged from three to nine, with a mean of 5.1. The polymorphic information content values ranged from 0.39 to 0.66, with a mean of 0.52. Thirteen primers amplified products indicating presence of A. juvenalis chromatin. The largest mean genetic similarity (0.84) with all other forms was shown by a strain derived from an A. juvenalis 6x × [(Lanca × L506/79) × CZR142/79] hybrid, while A. juvenalis was the least similar (0.33). We conclude that ISSRs can reliably identify A. juvenalis chromatin in the triticale background and efficiently estimate genetic similarity of hybrids and parental forms.

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Section: Discussionsupporting

confidence: 87%

Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs

Grądzielewska

Gruszecka²,

Leśniowska-Nowak³

et al. 2012

Genet. Mol. Res.

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“…To study the collection of barley and oat accessions a set of 26 microsatellite markers per each crop were chosen from several publications (Liu et al 1996;Russel et al 1997;Ramsay et al 2000;Li & Rossnagel 2000;Pal et al 2002). They are listed in Table 2 including repeat motif, annealing temperature and number of detected alleles per each microsatellite locus.…”

Section: Methodsmentioning

confidence: 99%

Genetic resources of barley and oat characterised by microsatellites

Leišová¹,

Kučera²,

Dotlačil³

2007

Czech J. Genet. Plant Breed.

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Barley (Hordeum vulgare L.) and oat (avena sativa L.) are important crop species. 1865 accessions of winter barley, 2707 accessions of spring barley and 1998 accessions of oat are maintained in RICP Gene bank. The expert core collection is used to be established as a tool for germplasm study, conservation of genetic variability and for the identification of useful genes. The main aim of this study was to evaluate genetic diversity of barley and oat genotypes within the expert core collections. Genetic variation of 176 barley accessions was analyzed using 26 microsatellite loci, covering all 6 chromosomes. 330 oat accessions were analyzed using 26 microsatellite loci that are mapped only into linkage groups. For 26 barley microsatellite loci, 328 alleles were detected. The average number of alleles per locus was 12.6. In oat, for 26 oat microsatellite loci, 353 alleles were detected. The average number of alleles per locus was 13.6. The average DI (diversity index) was 0.11 in barley and 0.09 in oat. Dendrogram and PCA (Principal Component Analysis) based on microsatellite data showed a different influence of the place of origin, age of variety and pedigree on grouping into clusters. PCA showed that the breeding process had a negative impact on the level of genetic diversity and therefore there is a necessity of barley and oat germplasm conservation.

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“…The seeds have sown in pots under laboratory conditions (15)(16)(17)(18)(19)(20). The leaflets have been collected for each genotype at stage of two leaflets and kept in sterile tubes, thereafter have been crushed by a Tissue Lyser machine for five minutes.…”

Section: Dna Isolatedmentioning

confidence: 99%

Assess the Degree of Genetic Divergence among Sixteen Complex Genetically Wheat Bread using Indicators SSR

Hamdalla¹

2017

IOSR JAVS

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The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars

Cited by 144 publications

References 34 publications

Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs

Identification of hybrids between triticale and Aegilops juvenalis (Thell.) Eig and determination of genetic similarity with ISSRs

Genetic resources of barley and oat characterised by microsatellites

Assess the Degree of Genetic Divergence among Sixteen Complex Genetically Wheat Bread using Indicators SSR

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