The Development of Multiple Stress-Resistant Cauliflower Using Mutagenesis in Conjunction With a Microshoot Tissue Culture Technique

Fuller, Michael P.; Eed, Manal Hassan

doi:10.17660/actahortic.2003.618.6

Cited by 10 publications

(8 citation statements)

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“…The work here reports the assessment of abiotic stress resistance of cauliflower lines following mutagenesis and hydroxyproline selection using this new protocol. Preliminary results have already been reported (Fuller and Eed 2003) and the current paper reports the results of a larger population of lines and their stability following up to 15 routine sub-cultures and 2 years storage at low temperature (5°C). With the larger volume of data generated it was possible to compare the responses of the lines both in vitro and in vivo and investigate correlations between cold, salt and hydroxyproline resistance and proline accumulation.…”

Section: Introductionmentioning

confidence: 77%

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Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

Fuller

Metwali

Eed

et al. 2006

Plant Cell Tiss Organ Cult

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An efficient in vitro screening method has been developed for cauliflower to create NEU and NMU induced mutant lines selected on hydroxyproline containing medium. Mutant lines and control plants were sub-cultured many times on maintenance medium and stored at 5°C for 2 years and then tested for salt and hydroxyproline resistance as in vitro and in vivo plants. In vitro shoot tips were also sub-cultured to media containing hydroxyproline and NaCl for 28 days and then assessed for their leaf proline content. Non-acclimated and acclimated in vivo plants were also assessed for resistance to freezing. Populations of control and selected lines were created by mass pollination and subsequently tested for their NaCl and frost resistance. Control plants had little or no NaCl or hydroxyproline resistance whilst selected plants showed varying degrees of resistance. In vitro and in vivo responses of selected lines were correlated. Leaf proline content was increased markedly in the mutant lines and the greatest proline contents occurred following NaCl stress with the most respondent line having 100-fold levels compared to the controls. Both non-acclimated and acclimated selected lines showed improved frost resistance over controls. Improvements in frost resistance were heritable but improvements in NaCl resistance were not. The results clearly demonstrated that NaCl, frost and hydroxyproline resistance were stable traits over repeated in vitro sub-cultures and prolonged low temperature storage. A complete range of mutants with single, double or triple resistance traits were produced. The level of resistance however was not necessarily correlated with the level of proline and some lines showed resistance without elevated proline. It is concluded that elevated proline is not essential for improved resistance to abiotic stress in cauliflower, but where it does occur it does improve resistance.

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Section: Introductionmentioning

confidence: 77%

“…A population of non-mutated/ selected control clones was also prepared from the same curd material. A number of clones were tested for their resistance to various stresses (Fuller and Eed 2003) and then maintained in vitro over a period of 2 years and reassessed for their resistance to various stresses.…”

Section: Mutagenesis and Selectionmentioning

confidence: 99%

Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

Fuller

Metwali

Eed

et al. 2006

Plant Cell Tiss Organ Cult

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“…These methods induce point mutations, deletions, or insertions, respectively and have been useful in breeding for biotic (Bhagwat & Duncan, 1998;Kowalski & Cassells, 1999) and abiotic (Fuller & Eed, 2003;Khan et al, 2001) stress in non-legume crops. In legumes most effort has occurred with nitrogen fixation (Sagan et al, 1994), and mutants with resistance or tolerance to stresses have not been described.…”

Section: Somaclonal Variation and In Vitro Mutagenesismentioning

confidence: 99%

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

et al. 2006

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Biotic and abiotic stresses cause significant yield losses in legumes and can significantly affect their productivity. Biotechnology tools such as marker-assisted breeding, tissue culture, in vitro mutagenesis and genetic transformation can contribute to solve or reduce some of these constraints. However, only limited success has been achieved so far. The emergence of "omic" technologies and the establishment of model legume plants such as Medicago truncatula and Lotus japonicus are promising strategies for understanding the molecular genetic basis of stress resistance, which is an important bottleneck for molecular breeding. Understanding the mechanisms that regulate the expression of stress-related genes is a fundamental issue in plant biology and will be necessary for the genetic improvement of legumes. In this review, we describe the current status of biotechnology approaches in relation to biotic and abiotic stresses in legumes and how these useful tools could be used to improve resistance to important constraints affecting legume crops.

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“…Both the osmolality of the leaf sap and frost resistance of the leaves were linearly correlated with the salt concentration in the hydroponics culture medium. In their investigations on the cauliflower, Fuller and Eed (2003) found that the most salt-resistant plants were also more frost tolerant.…”

Section: Average Chloropyll Content (Spad)mentioning

confidence: 98%

Influence of Salt Stress on Growth and Frost Resistance of Three Winter Cereals

Matuszak-Slamani¹,

Brzóstowicz²

2015

International Agrophysics

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A b s t r a c t. This paper presents results of a study on the influence of 0-150 mmol NaCl dm -3 Hoagland solution on growth, chlorophyll content, photosynthesis and frost resistance of seedlings of three winter cereals: wheat -cv. Almari, rye -cv. Amilo, and triticale -cv. Tornado. Sodium chloride at 25 mmol dm -3 caused better growth of wheat shoots and roots, both of fresh and dry matter. Higher concentrations of NaCl in the medium decreased the biomass of the tested seedlings. The influence of NaCl on the chlorophyll content in the seedlings varied. The conductometry method showed that the resistance of the cell walls of wheat and rye to low temperature decreased in the presence of NaCl in the growth medium. Luminescence has shown that seedlings that grew in NaCl-containing medium indicated an impediment of electron flow at a lower temperature than the control plants.

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The Development of Multiple Stress-Resistant Cauliflower Using Mutagenesis in Conjunction With a Microshoot Tissue Culture Technique

Cited by 10 publications

References 0 publications

Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

Evaluation of Abiotic Stress Resistance in Mutated Populations of Cauliflower (Brassica oleracea var. botrytis)

Biotechnology approaches to overcome biotic and abiotic stress constraints in legumes

Influence of Salt Stress on Growth and Frost Resistance of Three Winter Cereals

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