The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy

Yang, I‐Hsuan; Chen, Yo-Shen; Li, Jiajing; Liang, Ya‐Jyun; Lin, Tzu‐Chieh; Jakfar, Subhaini; Thacker, Minal; Wu, Shinn-Chih; Lin, Feng-Huei

doi:10.1016/j.bioactmat.2020.11.029

Cited by 18 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the concentration of cells in microspheres is too high, it can easily cause cell death due to hypoxia and nutrient deficiency; meanwhile, if the concentration of cells in microspheres is too low, cell survival and cell functions can be affected, resulting in reduced function of cellular microspheres. Previous studies have shown that a cell density of 10 6 /mL in sodium alginate microspheres exhibits good survival rates and functions [55,56]. As observed in our study, HepG2 cells preserved their viability no less than 80% over a course of 14 days and maintained biological functionality such as albumin generation and urea secretion (Figs.…”

Section: Discussionsupporting

confidence: 84%

Development of digital organ-on-a-chip to assess hepatotoxicity and extracellular vesicle-based anti-liver cancer immunotherapy

Shi

et al. 2022

Bio-des. Manuf.

View full text Add to dashboard Cite

Organ-on-a-chip systems have been increasingly recognized as attractive platforms to assess toxicity and to develop new therapeutic agents. However, current organ-on-a-chip platforms are limited by a “single pot” design, which inevitably requires holistic analysis and limits parallel processing. Here, we developed a digital organ-on-a-chip by combining a microwell array with cellular microspheres, which significantly increased the parallelism over traditional organ-on-a-chip for drug development. Up to 127 uniform liver cancer microspheres in this digital organ-on-a-chip format served as individual analytical units, allowing for analysis with high consistency and quick response. Our platform displayed evident anti-cancer efficacy at a concentration of 10 μM for sorafenib, and had greater alignment than the “single pot” organ-on-a-chip with a previous in vivo study. In addition, this digital organ-on-a-chip demonstrated the treatment efficacy of natural killer cell-derived extracellular vesicles for liver cancer at 50 μg/mL. The successful development of this digital organ-on-a-chip platform provides high-parallelism and a low-variability analytical tool for toxicity assessment and the exploration of new anticancer modalities, thereby accelerating the joint endeavor to combat cancer. Graphic abstract

show abstract

Section: Discussionsupporting

confidence: 84%

Development of digital organ-on-a-chip to assess hepatotoxicity and extracellular vesicle-based anti-liver cancer immunotherapy

Shi

et al. 2022

Bio-des. Manuf.

View full text Add to dashboard Cite

show abstract

“…Aluminum oxide (Al 2 O 3 , Sigma-Aldrich) and zinc diethyldithiocarbamate (ZDEC, Sigma-Aldrich) were used as negative and positive controls, respectively. The extracted medium was cultured with C2C12 myoblasts for 24 h. Thereafter, 100 μL WST-1 solution was added to the wells and incubated for 2 h. The absorbance values of each well were measured at 450 nm using an ELISA plate reader (Molecular Devices) [ 18 , 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Cytotoxicity was evaluated using the LIVE/DEAD staining kit (L3224, Invitrogen, USA) according to the manufacturer’s instructions [ 19 ]. C2C12 myoblasts were cultured in a 24-well plate at a density of 3 × 10 4 cells/well and incubated for 24 h for full adhesion.…”

Section: Methodsmentioning

confidence: 99%

Curcumin-Loaded Hydrophobic Surface-Modified Hydroxyapatite as an Antioxidant for Sarcopenia Prevention

et al. 2021

Self Cite

View full text Add to dashboard Cite

Oxidative stress and later-induced chronic inflammation have been reported to play an important role on the progression of sarcopenia. Current treatments for sarcopenia are mainly administered to patients whom sarcopenia already developed. However, there has been no promising results shown in therapy. Therefore, the development of therapeutic and preventive strategies against sarcopenia would be necessary. Curcumin is a traditional medicine that possesses anti-inflammatory and antioxidative properties. In the present study, hydroxyapatite was subjected to hydrophobic surface modifications for curcumin loading (Cur-SHAP). It was, subsequently, utilized for delivery to the patient’s body via intramuscular injection in order to achieve constant release for more than 2 weeks, preventing the progression of the sarcopenia or even leading to recovery from the early stage of the illness. According to the results of WST-1, LIVE/DEAD, DCFDA, and gene expression assays, Cur-SHAP exhibited good biocompatibility and showed great antioxidant/anti-inflammatory effects through the endocytic pathway. The results of the animal studies showed that the muscle endurance, grip strength, and fat/lean mass ratio were all improved in Cur-SHAP-treated rats from LPS-induced sarcopenia. In summary, we successfully synthesized hydrophobic surface modification hydroxyapatite for curcumin loading (Cur-SHAP) and drug delivery via the IM route. The LPS-induced sarcopenia rats were able to recover from disease after the Cur-SHAP treatment.

show abstract

“…The unique three-dimensional gel structure provides comfortable stereo space for the growth of the seed cells, and the shape is regular and the surface is smooth, which can avoid secondary damage to the injured site when the implant of irregular shape is implanted. It has been used in the cartilage [ 120 ], hard bone [ 121 ], nerve tissue repair [ 122 , 123 ] and other aspects [ 124 ].…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Preparation of Alginate-Based Biomaterials and Their Applications in Biomedicine

2021

View full text Add to dashboard Cite

Alginates are naturally occurring polysaccharides extracted from brown marine algae and bacteria. Being biocompatible, biodegradable, non-toxic and easy to gel, alginates can be processed into various forms, such as hydrogels, microspheres, fibers and sponges, and have been widely applied in biomedical field. The present review provides an overview of the properties and processing methods of alginates, as well as their applications in wound healing, tissue repair and drug delivery in recent years.

show abstract

The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy

Cited by 18 publications

References 52 publications

Development of digital organ-on-a-chip to assess hepatotoxicity and extracellular vesicle-based anti-liver cancer immunotherapy

Development of digital organ-on-a-chip to assess hepatotoxicity and extracellular vesicle-based anti-liver cancer immunotherapy

Curcumin-Loaded Hydrophobic Surface-Modified Hydroxyapatite as an Antioxidant for Sarcopenia Prevention

Preparation of Alginate-Based Biomaterials and Their Applications in Biomedicine

Contact Info

Product

Resources

About